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Emerging trends in prime editing for precision genome editing [PDF]
Experimental and Molecular MedicinePrime editing is an advanced genome editing technology that enables precise genetic modifications without inducing double-strand breaks or requiring donor DNA templates.
Jaesuk Lee, Jiyeon Kweon, Yongsub Kim
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It’s prime time for multiplexed prime editing [PDF]
Cell GenomicsPrime editing screens allow precise and scalable studies of genetic variants in their native genomic context but are limited by variable editing efficiency.
Ke Wu, Francisco J. Sánchez-Rivera
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The Development, Optimization and Future of Prime Editing
International Journal of Molecular Sciences, 2023 Prime editing is a rapidly developing method of CRISPR/Cas-based genome editing. The increasing number of novel PE applications and improved versions demands constant analysis and evaluation. The present review covers the mechanism of prime editing, the optimization of the method and the possible next step in the evolution of CRISPR/Cas9-associated ...
I O Petrova, Svetlana A Smirnikhina
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Current advancement in the application of prime editing
Frontiers in Bioengineering and Biotechnology, 2023 Prime editing (PE) is a precise genome manipulation technology based on the “search and replace” approach of the CRISPR-Cas9 system, while it does not require the exogenous donor DNA and the DNA double-strand breaks (DSBs).
Zhangrao Huang +4 more
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Development of Plant Prime-Editing Systems for Precise Genome Editing
Plant Communications, 2020 Prime-editing systems have the capability to perform efficient and precise genome editing in human cells. In this study, we first developed a plant prime editor 2 (pPE2) system and test its activity by generating a targeted mutation on an HPT-ATG ...
Rongfang Xu +5 more
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Successful In Vitro Modification of the Dmd Gene Using Prime Editing [PDF]
CellsDuchenne muscular dystrophy (DMD) is a fatal X-linked neuromuscular disorder caused by mutations in the dystrophin gene. Prime editing is a versatile genome editing technology capable of introducing precise nucleotide changes without generating double ...
Ayesha Siddika +3 more
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piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition
Frontiers in Genome Editing, 2021 Prime editing is a novel genome editing technology that allows a wide range of tailored genomic alterations. Prime editing does not involve homologous recombination, but suffers from low efficacy.
Jonas Holst Wolff +4 more
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CRISPR-Cas9 DNA Base-Editing and Prime-Editing [PDF]
International Journal of Molecular Sciences, 2020 Many genetic diseases and undesirable traits are due to base-pair alterations in genomic DNA. Base-editing, the newest evolution of clustered regularly interspaced short palindromic repeats (CRISPR)-Cas-based technologies, can directly install point-mutations in cellular DNA without inducing a double-strand DNA break (DSB).
Michelle E Mcclements +2 more
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HDAC inhibitors improve CRISPR-Cas9 mediated prime editing and base editing
Molecular Therapy: Nucleic Acids, 2022 Recent advances in CRISPR-Cas9 techniques, especially the discovery of base and prime editing, have significantly improved our ability to make precise changes in the genome.
Nan Liu +8 more
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Prime editing: advances and therapeutic applications
Trends in Biotechnology, 2023 Clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR-Cas)-mediated genome editing has revolutionized biomedical research and will likely change the therapeutic and diagnostic landscape. However, CRISPR-Cas9, which edits DNA by activating DNA double-strand break (DSB) repair pathways, is not always sufficient for gene ...
Peng Shang +2 more
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