Results 31 to 40 of about 93,364 (279)

Generation of AAVS1 integrated doxycycline-inducible CRISPR-Prime Editor human induced pluripotent stem cell line

Stem Cell Research, 2021
Prime editing uses the Cas9 nickase fused to a reverse transcriptase to copy a DNA sequence into a specific locus from a ‘prime editing’ guide RNA (pegRNA), eliminating the need for double-stranded DNA breaks and donor DNA templates.
Nike Bharucha, Jennifer Arthur Ataam, Alexandra A. Gavidia, Ioannis Karakikes   +3 more
doaj   +1 more source

One Prime for All Editing [PDF]

Cell, 2019
Many targeted base transversions, insertions, and deletions remain challenging due to the lack of precise and efficient genome editing technologies. Recently, Anzalone et al. reported a versatile approach to achieve all types of genome edits, shedding new light on correcting most genetic variants associated with diseases.
Li, Yang, Bei, Yang, Jia, Chen
openaire   +2 more sources

Prime Editing: An All-Rounder for Genome Editing

International Journal of Molecular Sciences, 2022
Prime editing (PE), as a “search-and-replace” genome editing technology, has shown the attractive potential of versatile genome editing ability, which is, in principle, currently superior to other well-established genome-editing technologies in the all-in-one operation scope.
Chenyu Lu, Jingyu Kuang, Tong Shao, Sisi Xie, Ming Li, Lingyun Zhu, Lvyun Zhu   +6 more
openaire   +2 more sources

Prime Editing: Precision Genome Editing by Reverse Transcription [PDF]

Molecular Cell, 2020
Genome editing is a method for making targeted sequence changes to the genomes of living cells. Prime editing, recently reported by Anzalone et al. (2019), is a new technology that uses reverse transcription to "write" programmed sequence changes into genomic DNA and thus promises significant technical advances.
Jun, Yan, Ann, Cirincione, Britt, Adamson   +2 more
openaire   +2 more sources

Prime editing-mediated correction of the CFTR W1282X mutation in iPSCs and derived airway epithelial cells.

PLoS ONE, 2023
A major unmet need in the cystic fibrosis (CF) therapeutic landscape is the lack of effective treatments for nonsense CFTR mutations, which affect approximately 10% of CF patients.
Chao Li, Zhong Liu, Justin Anderson, Zhongyu Liu, Liping Tang, Yao Li, Ning Peng, Jianguo Chen, Xueming Liu, Lianwu Fu, Tim M Townes, Steven M Rowe, David M Bedwell, Jennifer Guimbellot, Rui Zhao   +14 more
doaj   +1 more source

Prime Editing for Inherited Retinal Diseases [PDF]

Frontiers in Genome Editing, 2021
Inherited retinal diseases (IRDs) are chronic, hereditary disorders that lead to progressive degeneration of the retina. Disease etiology originates from a genetic mutation—inherited or de novo—with a majority of IRDs resulting from point mutations.
Bruna Lopes da Costa, Bruna Lopes da Costa, Sarah R. Levi, Eric Eulau, Yi-Ting Tsai, Yi-Ting Tsai, Peter M. J. Quinn   +6 more
openaire   +3 more sources

Prime editing for functional repair in patient-derived disease models [PDF]

, 2020
Prime editing is a recent genome editing technology using fusion proteins of Cas9-nickase and reverse transcriptase, that holds promise to correct the vast majority of genetic defects.
van der Laan, Luc; id_orcid, Joore, I.P., Joore, Indi P, Oka, R, van der Laan, Luc J W, Verstegen, M.M.A. (Monique), Nieuwenhuis, E.E.S. (Edward), Rurika Oka, van Vugt, A.H.M., van der Laan, Luc J. W., Joore, IP, Laan, L.J.W. (Luc) van der, van Hasselt, PM, Mokry, Michal, Schene, IF, van Boxtel, R, van Hasselt, Peter M., Nieuwenhuis, EES, Indi P. Joore, Fuchs, SA, van der Doef, H.P.J., Peter M. van Hasselt, Michal Mokry, Imre F. Schene, Sabine A. Fuchs, Edward E. S. Nieuwenhuis, Monique M. A. Verstegen, Joore, Indi P., van der Doef, HPJ, van der Doef, Hubert P J, Verstegen, Monique M. A., Verstegen, Monique M A, van Boxtel, Ruben, Verstegen, Monique; id_orcid, Mokry, M, Oka, Rurika, Fuchs, Sabine A., Ruben van Boxtel, van Vugt, AHM, Nieuwenhuis, Edward E. S., van Vugt, Anke H. M., Schene, Imre F., Hasselt, P.M. (Peter) van, Hubert P. J. van der Doef, van Vugt, Anke H M, Verstegen, Monique, Fuchs, Sabine A, van der Doef, Hubert P. J., van der Laan, Luc, Luc J. W. van der Laan, van Hasselt, Peter M, Anke H. M. van Vugt, Nieuwenhuis, Edward E S, Oka, R., Boxtel, R. (Ria) van, Schene, Imre F   +55 more
core   +1 more source

Peptide fusion improves prime editing efficiency

Nature Communications, 2022
Prime editing enables search-and-replace genome editing but is limited by low editing efficiency. Here the authors present PepSEq, a high-throughput method for screening a large library of peptides that influence prime editing efficiency.
Minja Velimirovic, Larissa C. Zanetti, Max W. Shen, James D. Fife, Lin Lin, Minsun Cha, Ersin Akinci, Danielle Barnum, Tian Yu, Richard I. Sherwood   +9 more
doaj   +1 more source

PINE-TREE enables highly efficient genetic modification of human cell lines

Molecular Therapy: Nucleic Acids, 2023
Prime editing technologies enable precise genome editing without the caveats of CRISPR nuclease-based methods. Nonetheless, current approaches to identify and isolate prime-edited cell populations are inefficient.
Carlye Frisch, William W. Kostes, Brooke Galyon, Brycelyn Whitman, Stefan J. Tekel, Kylie Standage-Beier, Gayathri Srinivasan, Xiao Wang, David A. Brafman   +8 more
doaj   +1 more source

In vivo prime editing rescues alternating hemiplegia of childhood in mice [PDF]

Cell
Laura C Anderson, Linda C Laux, Sylvie Forlani   +2 more
exaly   +2 more sources