Results 21 to 30 of about 93,364 (279)
BacPE: a versatile prime-editing platform in bacteria by inhibiting DNA exonucleases
Nature CommunicationsPrime editing allows precise installation of any single base substitution and small insertions and deletions without requiring homologous recombination or double-strand DNA breaks in eukaryotic cells.
Hongyuan Zhang +10 more
doaj +2 more sources
Molecular Therapy: Nucleic AcidsPrime editors are CRISPR-based genome engineering tools with significant potential for rectifying patient mutations. However, their usage requires experimental optimization of the prime editing guide RNA (PegRNA) to achieve high editing efficiency.
Roghayyeh Alipanahi +2 more
doaj +2 more sources
Dual inhibition of DNA-PK and Polϴ boosts precision of diverse prime editing systems
Nature CommunicationsPrime editing is a genome engineering tool that allows installation of various small edits with high precision. However, prime editing efficiency and purity can vary widely across different edits, genomic targets, and cell types.
Louis C. Dacquay +12 more
doaj +2 more sources
Precise correction of G6PD Viangchan mutation in iPSCs by prime editing strategy [PDF]
Scientific ReportsIndividuals with glucose-6-phosphate dehydrogenase (G6PD) deficiency hold a significant risk of severe hemolytic crises under oxidative stress. Currently, the definitive and curative treatment for the disorder has not been developed.
Chaiwat Netsawang +3 more
doaj +2 more sources
eLife, 2022 Prime editing is a recently developed CRISPR/Cas9 based gene engineering tool that allows the introduction of short insertions, deletions, and substitutions into the genome. However, the efficiency of prime editing, which typically achieves editing rates
Dorottya Anna Simon +6 more
doaj +1 more source
Frontiers in Genome Editing, 2023 Prime editing of human hematopoietic stem cells has the potential to become a safe and efficient way of treating diseases of the blood directly in patients.
Jonas Holst Wolff, Jacob Giehm Mikkelsen
doaj +1 more source
Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize
Genome Biology, 2020 Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing technology that has been recently developed. However, low efficiency of prime editing has been shown in transgenic rice lines.
Yuan-Yuan Jiang +10 more
doaj +1 more source
Chromatin context-dependent regulation and epigenetic manipulation of prime editing [PDF]
, 2023 Prime editing is a powerful means of introducing precise changes to specific locations in mammalian genomes. However, the widely varying efficiency of prime editing across target sites of interest has limited its adoption in the context of both basic ...
Chen, Wei +11 more
core +1 more source
Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor
Genome Biology, 2021 Prime editors, novel genome-editing tools consisting of a CRISPR-Cas9 nickase and an engineered reverse transcriptase, can induce targeted mutagenesis. Nevertheless, much effort is required to optimize and improve the efficiency of prime-editing. Herein,
Soo-Ji Park +11 more
doaj +1 more source
Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair
Nature Communications, 2022 Prime Editing is a versatile genome engineering tool. Here, the authors identify the DNA repair pathway known as mismatch repair as inhibitory for Prime Editing, thus, loss of mismatch repair enhances the efficiency of Prime Editing.
J. Ferreira da Silva +7 more
doaj +1 more source