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CRISPR–Cas9 Structures and Mechanisms
Annual Review of Biophysics, 2017 Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 to defend against invading phages and conjugative plasmids by introducing site-specific double-stranded breaks in target DNA.
Jennifer Doudna, Fuguo Jiang
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The Advance of CRISPR-Cas9-Based and NIR/CRISPR-Cas9-Based Imaging System [PDF]
Frontiers in Chemistry, 2021 The study of different genes, chromosomes and the spatiotemporal relationship between them is of great significance in the field of biomedicine. CRISPR-Cas9 has become the most widely used gene editing tool due to its excellent targeting ability.
Huanhuan Qiao +6 more
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Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation
Nature Reviews Molecular Cell Biology, 2015 The bacterial CRISPR–Cas9 system has emerged as a multifunctional platform for sequence-specific regulation of gene expression. This Review describes the development of technologies based on nuclease-deactivated Cas9, termed dCas9, for RNA-guided genomic
Lei S Qi +2 more
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Therapeutic applications of CRISPR-Cas9 gene editing [PDF]
Frontiers in Genome EditingCRISPR-Cas9 is a gene editing tool used extensively in biological research that is now making its way into clinical therapies. With the first CRISPR therapy obtaining approval by the United States’ Food and Drug Administration (FDA) in late 2023, we look
Aditya Bharti, Joann Mudge
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CRISPR/Cas9 Delivery System Engineering for Genome Editing in Therapeutic Applications
Pharmaceutics, 2021 The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein 9 (CRISPR/Cas9) systems have emerged as a robust and versatile genome editing platform for gene correction, transcriptional regulation, disease modeling, and ...
Hao Cheng, Feng Zhang, Yang Ding
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Application of CRISPR/Cas9 Technology in Cancer Treatment: A Future Direction
Current Oncology, 2023 Gene editing, especially with clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9), has advanced gene function science. Gene editing’s rapid advancement has increased its medical/clinical value.
Ali A. Rabaan +20 more
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Veterinarska stanica, 2021 Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR- associated system (Cas) is a system that provides immunity to most prokaryoticorganisms against viral attacks and other foreign bodies. CRISPR systems consist of a scissor-like protein called Cas9 and a genetic GPS guide “The guide RNA”.
Mimoune, Nora +4 more
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CRISPR/CAS9 Technologies [PDF]
Journal of Bone and Mineral Research, 2017 ABSTRACT The Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) pathway is revolutionizing biological research. Modifications to this primitive prokaryotic immune system now enable scientists to efficiently edit DNA or modulate gene expression in living ...
Bart O, Williams, Matthew L, Warman
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Current applications and future perspective of CRISPR/Cas9 gene editing in cancer
Molecular Cancer, 2022 Clustered regularly interspaced short palindromic repeats (CRISPR) system provides adaptive immunity against plasmids and phages in prokaryotes. This system inspires the development of a powerful genome engineering tool, the CRISPR/CRISPR-associated ...
Si-Wei Wang +9 more
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