AcrIIA5 Inhibits a Broad Range of Cas9 Orthologs by Preventing DNA Target Cleavage
Cell Reports, 2019 Summary: CRISPR-Cas9 is an adaptive immune system for prokaryotes to defend against invasive genetic elements such as phages and has been used as a powerful tool for genome editing and modulation.
Guoxu Song +6 more
doaj +1 more source
Application of CRISPR/Cas9 technology in forages
Grassland Research, 2022 Genome editing is an advanced genetic modification tool that facilitates the removal, insertion, or substitution of nucleotides in the genome of an organism for mutation induction.
Qinyan Bao +6 more
doaj +1 more source
Development of delivery strategies for CRISPR‐Cas9 genome editing
BMEMat, 2023 The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR‐related protein 9 (Cas9) genome editing system has attracted much attention due to its powerful genome editing capacity. However, CRISPR‐Cas9 components are easily degraded
Qi Liu +4 more
doaj +1 more source
Advancing chimeric antigen receptor T cell therapy with CRISPR/Cas9
Protein & Cell, 2017 The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (CRISPR/Cas9) system, an RNA-guided DNA targeting technology, is triggering a revolution in the field of biology.
Jiangtao Ren, Yangbing Zhao
doaj +1 more source
Anti-CRISPR AcrIIA5 Potently Inhibits All Cas9 Homologs Used for Genome Editing
Cell Reports, 2019 Summary: CRISPR-Cas9 systems provide powerful tools for genome editing. However, optimal employment of this technology will require control of Cas9 activity so that the timing, tissue specificity, and accuracy of editing may be precisely modulated.
Bianca Garcia +13 more
doaj +1 more source
The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit [PDF]
G3 Genes|Genomes|Genetics, 2021 ABSTRACT The genetic tractability of the yeast Saccharomyces cerevisiae has made it a key model organism for basic research and a target for metabolic engineering. To streamline the introduction of tagged genes and compartmental markers with powerful CRISPR-Cas9-based genome editing tools we constructed a Markerless Yeast Localization ...
Björn D M Bean +2 more
openaire +2 more sources
CRISPR interference : a structural perspective [PDF]
, 2013 This article was made open access through BIS OA funding. The laboratory is funded by grants from the Biotechnology and Biological Sciences Research Council (BBSRC).CRISPR (cluster of regularly interspaced palindromic repeats) is a prokaryotic adaptive ...
Naismith, Jim +2 more
core +1 more source
Production of CRISPR/Cas9-Mediated Self-Cleaving Helper-Dependent Adenoviruses
Molecular Therapy: Methods & Clinical Development, 2019 Prolonged expression of CRISPR/Cas9 raises concerns about off-target cleavage, cytotoxicity, and immune responses. To address these issues, we have developed a system to produce helper-dependent adenoviruses that express CRISPR/Cas9 to direct cleavage of
Donna J. Palmer +2 more
doaj +1 more source
A review on genome editing by CRISPR-Cas9 technique for cancer treatment [PDF]
, 2020 In this review, we summarize CRISPR-Cas9 system-based gene modification for the therapeutic treatment of cancer. Cancer is a group of diseases involving anomalous cell growth with the potential to invade or blow out to other parts of the body, which is ...
N. Chandra Barman +3 more
core +1 more source
CRISPR-Cas9-mediated Large Cluster Deletion and Multiplex Genome Editing in Paenibacillus polymyxa
, 2021 The use of molecular tools based on the clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) systems has rapidly advanced genetic engineering.
Christa Teckentrup (11846603) +2 more
core +2 more sources