Results 31 to 40 of about 243,751 (303)

CRISPR-mediated targeted mRNA degradation in the archaeon Sulfolobus solfataricus [PDF]

, 2014
European SulfoSYS-project [SysMo P–N-01-09-23] and grant [9P23000 and P25369] by the Austrian Research fund (to C.S.) and by grant [BB/K000314/1] from the Biotechnology and Biological Sciences Research Council (to M.F.W.). Funding for open access charge:
Manica, A., Zebec, Z., Schleper, C., Zhang, J., White, M.F.   +4 more
core   +1 more source

The MyLo CRISPR-Cas9 Toolkit: A Markerless Yeast Localization and Overexpression CRISPR-Cas9 Toolkit [PDF]

G3 Genes|Genomes|Genetics, 2021
ABSTRACT The genetic tractability of the yeast Saccharomyces cerevisiae has made it a key model organism for basic research and a target for metabolic engineering. To streamline the introduction of tagged genes and compartmental markers with powerful CRISPR-Cas9-based genome editing tools we constructed a Markerless Yeast Localization ...
Björn D M Bean, Malcolm Whiteway, Vincent J J Martin   +2 more
openaire   +2 more sources

Anti-CRISPR AcrIIA5 Potently Inhibits All Cas9 Homologs Used for Genome Editing

Cell Reports, 2019
Summary: CRISPR-Cas9 systems provide powerful tools for genome editing. However, optimal employment of this technology will require control of Cas9 activity so that the timing, tissue specificity, and accuracy of editing may be precisely modulated.
Bianca Garcia, Jooyoung Lee, Alireza Edraki, Yurima Hidalgo-Reyes, Steven Erwood, Aamir Mir, Chantel N. Trost, Uri Seroussi, Sabrina Y. Stanley, Ronald D. Cohn, Julie M. Claycomb, Erik J. Sontheimer, Karen L. Maxwell, Alan R. Davidson   +13 more
doaj   +1 more source

Cell cycle arrest and p53 prevent ON-target megabase-scale rearrangements induced by CRISPR-Cas9

, 2023
International audienceThe CRISPR-Cas9 system has revolutionized our ability to precisely modify the genome and has led to gene editing in clinical applications.
G. Cullot, J. M. Blouin, S. Amintas, A. Bedel, Bedel, A., Rosier, J., Laharanne, E., S. Fayet, Cappellen, D., E. Richard, Prat, F., F. Prat, D. Cappellen, Moreau-Gaudry, F., Blouin, J, M, Guyonnet-Duperat, V., J. Boutin, Bouron, J., J. Rosier, C. Thibault, Dabernat, S., J. Bouron, S. Dabernat, Boutin, J., V. Guyonnet-Duperat, Amintas, S., I. Lamrissi, P. Pennamen, Cullot, G., F. Moreau-Gaudry, J. P. Merlio, Moranvillier, I., Pennamen, P., Fayet, S., Lamrissi, I., I. Moranvillier, Merlio, J, P, Richard, E., Thibault, C., E. Laharanne   +39 more
core   +1 more source

Multiple nucleic acid cleavage modes in divergent type III CRISPR systems [PDF]

, 2016
CRISPR-Cas is an RNA-guided adaptive immune system that protects bacteria and archaea from invading nucleic acids. Type III systems (Cmr, Csm) have been shown to cleave RNA targets in vitro and some are capable of transcription-dependent DNA targeting ...
Zhang, Jing, White, Malcolm Frederick, Tello, Agnes, Liu, Huanting, Graham, Shirley   +4 more
core   +1 more source

Two-Metal Ion Mechanism of DNA Cleavage in CRISPR-Cas9 [PDF]

, 2019
CRISPR-Cas9 is a cutting-edge genome-editing technology, which employs the endonuclease Cas9 to cleave DNA sequences of interest. However, the catalytic mechanism of DNA cleavage and the critical role of the Mg2+ ions have remained elusive. Here, quantum–
Lorenzo, Casalino, Giulia, Palermo, Martin, Jinek   +2 more
core   +2 more sources

Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition [PDF]

, 2015
This work was supported by a grant from the Biotechnology and Biological Sciences Research Council (REF: BB/M000400/1 to MFW).The adaptive prokaryotic immune system CRISPR-Cas provides RNA-mediated protection from invading genetic elements.
Bolt, Edward, White, Malcolm F., Rollie, C, White, Malcolm F, Anna Sophie Brinkmann, Malcolm F White, Clare Rollie, Edward L Bolt, Brinkmann, Anna Sophie, Rollie, Clare, Bolt, EL, Rollie, Clare Jane Catherine, Schneider, Stefanie, White, MF, Stefanie Schneider, Bolt, Edward L., Schneider, S, Brinkmann, AS   +17 more
core   +1 more source

Nanoparticles for CRISPR–Cas9 delivery [PDF]

Nature Biomedical Engineering, 2017
The DNA mutation that causes Duchenne muscular dystrophy in mice can be corrected, with minimal off-target effects, by gold nanoparticles carrying the CRISPR components.
Zachary, Glass, Yamin, Li, Qiaobing, Xu
openaire   +2 more sources

Using local chromatin structure to improve CRISPR/Cas9 efficiency in zebrafish. [PDF]

PLoS ONE, 2017
Although the CRISPR/Cas9 has been successfully applied in zebrafish, considerable variations in efficiency have been observed for different gRNAs. The workload and cost of zebrafish mutant screening is largely dependent on the mutation rate of injected ...
Yunru Chen, Shiyang Zeng, Ruikun Hu, Xiangxiu Wang, Weilai Huang, Jiangfang Liu, Luying Wang, Guifen Liu, Ying Cao, Yong Zhang   +9 more
doaj   +1 more source

Overview of CRISPR–Cas9 Biology [PDF]

Cold Spring Harbor Protocols, 2016
Prokaryotes use diverse strategies to improve fitness in the face of different environmental threats and stresses, including those posed by mobile genetic elements (e.g., bacteriophages and plasmids). To defend against these elements, many bacteria and archaea use elegant, RNA-directed, nucleic acid–targeting adaptive restriction machineries called ...
Hannah K, Ratner, Timothy R, Sampson, David S, Weiss   +2 more
openaire   +2 more sources