Results 21 to 30 of about 254,775 (306)
Application of CRISPR/Cas9 technology in forages
Grassland Research, 2022 Genome editing is an advanced genetic modification tool that facilitates the removal, insertion, or substitution of nucleotides in the genome of an organism for mutation induction.
Qinyan Bao +6 more
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Frontiers in Plant Science, 2022 Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 is the third generation of novel targeted genome editing technology after zinc finger nucleases (ZFNs) and transcription activator like effector nucleases (TALENs). It is also one of
Yuchun Rao +4 more
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Genome engineering using the CRISPR-Cas9 system
Nature Protocols, 2013 Targeted nucleases are powerful tools for mediating genome alteration with high precision. The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short palindromic repeats (CRISPR) adaptive immune system can be used to facilitate
F. Ran +6 more
semanticscholar +1 more source
AcrIIA5 Inhibits a Broad Range of Cas9 Orthologs by Preventing DNA Target Cleavage
Cell Reports, 2019 Summary: CRISPR-Cas9 is an adaptive immune system for prokaryotes to defend against invasive genetic elements such as phages and has been used as a powerful tool for genome editing and modulation.
Guoxu Song +6 more
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Advancing chimeric antigen receptor T cell therapy with CRISPR/Cas9
Protein & Cell, 2017 The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (CRISPR/Cas9) system, an RNA-guided DNA targeting technology, is triggering a revolution in the field of biology.
Jiangtao Ren, Yangbing Zhao
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Development and Applications of CRISPR-Cas9 for Genome Engineering
Cell, 2014 Recent advances in genome engineering technologies based on the CRISPR-associated RNA-guided endonuclease Cas9 are enabling the systematic interrogation of mammalian genome function. Analogous to the search function in modern word processors, Cas9 can be
P. Hsu, E. Lander, Feng Zhang
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Development of delivery strategies for CRISPR‐Cas9 genome editing
BMEMat, 2023 The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR‐related protein 9 (Cas9) genome editing system has attracted much attention due to its powerful genome editing capacity. However, CRISPR‐Cas9 components are easily degraded
Qi Liu +4 more
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Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
Science, 2013 Improving Whole-Genome Screens Improved methods are needed for the knockout of individual genes in genome-scale functional screens. Wang et al. (p. 80, published online 12 December) and Shalem et al. (p.
Ophir Shalem +10 more
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Production of CRISPR/Cas9-Mediated Self-Cleaving Helper-Dependent Adenoviruses
Molecular Therapy: Methods & Clinical Development, 2019 Prolonged expression of CRISPR/Cas9 raises concerns about off-target cleavage, cytotoxicity, and immune responses. To address these issues, we have developed a system to produce helper-dependent adenoviruses that express CRISPR/Cas9 to direct cleavage of
Donna J. Palmer +2 more
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Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
Cell, 2013 Targeted genome editing technologies have enabled a broad range of research and medical applications. The Cas9 nuclease from the microbial CRISPR-Cas system is targeted to specific genomic loci by a 20-nt guide sequence, which can tolerate certain ...
F. Ran +12 more
semanticscholar +1 more source