Results 11 to 20 of about 243,751 (303)
CRISPR/Cas9 genetic screens in hepatocellular carcinoma gene discovery
Current Research in Biotechnology, 2023 The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a powerful gene editing tool originated from prokaryotes.
Cynthia H. Chiu
doaj +1 more source
CRISPR/Cas9 in Gastrointestinal Malignancies [PDF]
Frontiers in Cell and Developmental Biology, 2021 Gastrointestinal (GI) cancers such as colorectal cancer (CRC), gastric cancer (GC), esophageal cancer (EG), pancreatic duct adenocarcinoma (PDAC) or hepatocellular cancer (HCC) belong to the most commonly diagnosed types of cancer and are among the most frequent causes of cancer related death worldwide.
Jefremow, André +2 more
openaire +4 more sources
Stimuli-responsive nanoformulations for CRISPR-Cas9 genome editing
Journal of Nanobiotechnology, 2022 The CRISPR-Cas9 technology has changed the landscape of genome editing and has demonstrated extraordinary potential for treating otherwise incurable diseases. Engineering strategies to enable efficient intracellular delivery of CRISPR-Cas9 components has
Tianxu Fang +3 more
doaj +1 more source
Epigenetic switch reveals CRISPR/Cas9 response to cytosine methylation in plants: Comment [PDF]
, 2022 Commentary on Přibylová et al. (2022). 235 p 2285-2299 Genome editing techniques, such as the CRISPR/Cas9 system, offer a game-changing opportunity for crop improvement by enabling precise modifications to be made at targeted genomic loci.
Kaur, N., Halford, N. G., Raffan, S.
core +1 more source
Exosome-mediated delivery of CRISPR/Cas9 for targeting of oncogenic Kras G12D in pancreatic cancer
Life Science Alliance, 2021 This work identifies the use of exosomes to specifically deliver CRISPR/Cas9 to target oncogenic KrasG12D mutation in pancreatic cancer as a nonviral therapeutic strategy. CRISPR/Cas9 is a promising technology for gene editing.
Kathleen M McAndrews +5 more
doaj +1 more source
P1 Bacteriophage-Enabled Delivery of CRISPR-Cas9 Antimicrobial Activity Against Shigella flexneri [PDF]
, 2023 The discovery of clustered, regularly interspaced, short palindromic repeats (CRISPR) and the Cas9 RNA-guided nuclease provides unprecedented opportunities to selectively kill specific populations or species of bacteria.
Serge Mostowy +7 more
core +1 more source
BioMed Research International, 2022 CRISPR/Cas9 technology has become the most examined gene editing technology in recent years due to its simple design, yet low cost, high efficiency, and simple operation, which can also achieve simultaneous editing of multiple loci. It can also be carried out without using plasmids, saving lots of troubles caused by plasmids.
openaire +2 more sources
Truncated gRNA reduces CRISPR/Cas9-mediated off-target rate for MSTN gene knockout in bovines
Journal of Integrative Agriculture, 2019 The CRISPR/Cas9 mediates efficient gene editing but has off-target effects inconducive to animal breeding. In this study, the efficacy of CRISPR/Cas9 vectors containing different lengths of gRNA in reduction of the off-target phenomenon in the bovine ...
Zheng-wei ZHOU +7 more
doaj +1 more source
Rapid generation of endogenously driven transcriptional reporters in cells through CRISPR/Cas9 [PDF]
, 2015 CRISPR/Cas9 technologies have been employed for genome editing to achieve gene knockouts and knock-ins in somatic cells. Similarly, certain endogenous genes have been tagged with fluorescent proteins. Often, the detection of tagged proteins requires high
Rojas-Fernandez, Alejandro +5 more
core +1 more source
Application of CRISPR/Cas9 technology in forages
Grassland Research, 2022 Genome editing is an advanced genetic modification tool that facilitates the removal, insertion, or substitution of nucleotides in the genome of an organism for mutation induction.
Qinyan Bao +6 more
doaj +1 more source