Results 41 to 50 of about 248,807 (309)
Frontiers in Plant Science, 2020 For the past 5 years, clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) technology has appeared in the molecular biology research spotlight.
Yuan Zhang +3 more
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CRISPR/Cas9 system: recent applications in immuno-oncology and cancer immunotherapy
Experimental Hematology & Oncology, 2023 Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is essentially an adaptive immunity weapon in prokaryotes against foreign DNA.
Chen Chen, Zehua Wang, Yanru Qin
doaj +1 more source
Spontaneous Embedding of DNA Mismatches Within the RNA:DNA Hybrid of CRISPR-Cas9. [PDF]
, 2020 CRISPR-Cas9 is the forefront technology for editing the genome. In this system, the Cas9 protein is programmed with guide RNAs to process DNA sequences that match the guide RNA forming an RNA:DNA hybrid structure.
Hsu, Rohaine V +5 more
core
Integrate CRISPR/Cas9 for protein expression of HLA-B*38:68Q via precise gene editing. [PDF]
, 2019 The determination of null- or low-expressed HLA alleles is clinically relevant in both hematopoietic stem cell transplantation and solid organ transplantation.
Reed, Elaine F +2 more
core +1 more source
CRISPR/Cas9—Advancing Orthopoxvirus Genome Editing for Vaccine and Vector Development
Viruses, 2018 The clustered regularly interspaced short palindromic repeat (CRISPR)/associated protein 9 (Cas9) technology is revolutionizing genome editing approaches.
Arinze Okoli +3 more
doaj +1 more source
Scientific Reports, 2022 The CRISPR-Cas system is widely used for genome editing of cultured cells and organisms. The discovery of a new single RNA-guided endonuclease, CRISPR-Cas12a, in addition to the conventional CRISPR-Cas9 has broadened the number of editable target sites ...
Riki Ishibashi +4 more
doaj +1 more source
Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi. [PDF]
, 2015 Gene knockout strategies, RNAi and rescue experiments are all employed to study mammalian gene function. However, the disadvantages of these approaches include: loss of function adaptation, reduced viability and gene overexpression that rarely matches ...
Dowdy, Steven F +5 more
core +1 more source
Molecular Therapy: Nucleic Acids, 2020 Reticuloendotheliosis virus (REV) is an avian retrovirus that causes an oncogenic, immunosuppressive, and runting-stunting syndrome in avian hosts. The co-infection of REV and Marek’s disease virus (MDV), an oncogenic herpesvirus in chickens, further ...
Kai Li +12 more
doaj +1 more source
Frontiers in Genome Editing, 2022 The nuclease activity of the CRISPR-Cas9 system relies on the delivery of a CRISPR-associated protein 9 (Cas9) and a single guide RNA (sgRNA) against the target gene.
Karim E. Shalaby +6 more
doaj +1 more source
Knocking out barriers to engineered cell activity. [PDF]
, 2020 CRISPR-Cas9 gene-edited T cells show safety and long-term engraftment in ...
Doudna, Jennifer A, Hamilton, Jennifer R
core +1 more source