Results 41 to 50 of about 216,936 (304)
Frontiers in Marine Science, 2021 The Fujian oyster (Crassostrea angulate) is an important marine bivalve mollusk with high economic value. Gene function research and gene editing techniques have broad application prospects in oyster. The clustered regularly interspaced short palindromic
Kaidi Jin +11 more
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Generation of ADCK1 knock-out cells by CRISPR/Cas9 [PDF]
, 2022 openIntroduzione Il sistema CRISPR-Cas9 è una tecnica di editing genetico che si basa sull’utilizzo di una guida a singolo filamento di RNA (single-guide RNA, sgRNA) specifica, che guida l’endonucleasi Cas9 verso una regione di DNA target affinche ...
ZAMPIERI, SARA
core
Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition [PDF]
, 2015 This work was supported by a grant from the Biotechnology and Biological Sciences Research Council (REF: BB/M000400/1 to MFW).The adaptive prokaryotic immune system CRISPR-Cas provides RNA-mediated protection from invading genetic elements.
Bolt, Edward +17 more
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CRISPR/Cas9, a universal tool for genomic engineering
Вавиловский журнал генетики и селекции, 2016 The CRISPR/Cas9 system was initially described as an element of archeal and bacterial immunity, but gained much attention recently for its outstanding ability to be programmed to target any genomic loci through a short 20-nucleotide sgRNA region. Here we
A. V. Smirnov +3 more
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Expanding application of CRISPR-Cas9 system in microorganisms
Synthetic and Systems Biotechnology, 2020 The development of CRISPR-Cas9 based genetic manipulation tools represents a huge breakthrough in life sciences and has been stimulating research on metabolic engineering, synthetic biology, and systems biology.
Jing Zhao, Huan Fang, Dawei Zhang
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Using local chromatin structure to improve CRISPR/Cas9 efficiency in zebrafish. [PDF]
PLoS ONE, 2017 Although the CRISPR/Cas9 has been successfully applied in zebrafish, considerable variations in efficiency have been observed for different gRNAs. The workload and cost of zebrafish mutant screening is largely dependent on the mutation rate of injected ...
Yunru Chen +9 more
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Advancements and Obstacles of CRISPR-Cas9 Technology in Translational Research
Molecular Therapy: Methods & Clinical Development, 2019 The expanding CRISPR-Cas9 technology is an easily accessible, programmable, and precise gene-editing tool with numerous applications, most notably in biomedical research.
Liting You +5 more
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mSphere, 2020 Most of our knowledge relating to molecular mechanisms of human fungal pathogenesis in Candida albicans relies on reverse genetics approaches, requiring strain engineering. DNA-mediated transformation of C. albicans has been described as highly mutagenic,
Timea Marton +3 more
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CRISPR/Cas9 system: recent applications in immuno-oncology and cancer immunotherapy
Experimental Hematology & Oncology, 2023 Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is essentially an adaptive immunity weapon in prokaryotes against foreign DNA.
Chen Chen, Zehua Wang, Yanru Qin
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Overview of CRISPR–Cas9 Biology [PDF]
Cold Spring Harbor Protocols, 2016 Prokaryotes use diverse strategies to improve fitness in the face of different environmental threats and stresses, including those posed by mobile genetic elements (e.g., bacteriophages and plasmids). To defend against these elements, many bacteria and archaea use elegant, RNA-directed, nucleic acid–targeting adaptive restriction machineries called ...
Hannah K, Ratner +2 more
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