Results 41 to 50 of about 243,751 (303)
CRISPR-Cas9-mediated Large Cluster Deletion and Multiplex Genome Editing in Paenibacillus polymyxa
, 2021 The use of molecular tools based on the clustered regularly interspaced short palindromic repeats-Cas (CRISPR-Cas) systems has rapidly advanced genetic engineering.
Christa Teckentrup (11846603) +5 more
core +2 more sources
CRISPR/Cas9, a universal tool for genomic engineering
Вавиловский журнал генетики и селекции, 2016 The CRISPR/Cas9 system was initially described as an element of archeal and bacterial immunity, but gained much attention recently for its outstanding ability to be programmed to target any genomic loci through a short 20-nucleotide sgRNA region. Here we
A. V. Smirnov +3 more
doaj +1 more source
Expanding application of CRISPR-Cas9 system in microorganisms
Synthetic and Systems Biotechnology, 2020 The development of CRISPR-Cas9 based genetic manipulation tools represents a huge breakthrough in life sciences and has been stimulating research on metabolic engineering, synthetic biology, and systems biology.
Jing Zhao, Huan Fang, Dawei Zhang
doaj +1 more source
mSphere, 2020 Most of our knowledge relating to molecular mechanisms of human fungal pathogenesis in Candida albicans relies on reverse genetics approaches, requiring strain engineering. DNA-mediated transformation of C. albicans has been described as highly mutagenic,
Timea Marton +3 more
doaj +1 more source
Generation of ADCK1 knock-out cells by CRISPR/Cas9 [PDF]
, 2022 openIntroduzione Il sistema CRISPR-Cas9 è una tecnica di editing genetico che si basa sull’utilizzo di una guida a singolo filamento di RNA (single-guide RNA, sgRNA) specifica, che guida l’endonucleasi Cas9 verso una regione di DNA target affinche ...
ZAMPIERI, SARA
core
A review on genome editing by CRISPR-Cas9 technique for cancer treatment [PDF]
, 2020 In this review, we summarize CRISPR-Cas9 system-based gene modification for the therapeutic treatment of cancer. Cancer is a group of diseases involving anomalous cell growth with the potential to invade or blow out to other parts of the body, which is ...
N. Chandra Barman +3 more
core +2 more sources
CRISPR/Cas9—Advancing Orthopoxvirus Genome Editing for Vaccine and Vector Development
Viruses, 2018 The clustered regularly interspaced short palindromic repeat (CRISPR)/associated protein 9 (Cas9) technology is revolutionizing genome editing approaches.
Arinze Okoli +3 more
doaj +1 more source
Molecular Therapy: Nucleic Acids, 2020 Reticuloendotheliosis virus (REV) is an avian retrovirus that causes an oncogenic, immunosuppressive, and runting-stunting syndrome in avian hosts. The co-infection of REV and Marek’s disease virus (MDV), an oncogenic herpesvirus in chickens, further ...
Kai Li +12 more
doaj +1 more source
CRISPR/Cas9 system: recent applications in immuno-oncology and cancer immunotherapy
Experimental Hematology & Oncology, 2023 Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is essentially an adaptive immunity weapon in prokaryotes against foreign DNA.
Chen Chen, Zehua Wang, Yanru Qin
doaj +1 more source
Scientific Reports, 2022 The CRISPR-Cas system is widely used for genome editing of cultured cells and organisms. The discovery of a new single RNA-guided endonuclease, CRISPR-Cas12a, in addition to the conventional CRISPR-Cas9 has broadened the number of editable target sites ...
Riki Ishibashi +4 more
doaj +1 more source