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Expanding application of CRISPR-Cas9 system in microorganisms
Synthetic and Systems Biotechnology, 2020 The development of CRISPR-Cas9 based genetic manipulation tools represents a huge breakthrough in life sciences and has been stimulating research on metabolic engineering, synthetic biology, and systems biology.
Jing Zhao, Huan Fang, Dawei Zhang
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mSphere, 2020 Most of our knowledge relating to molecular mechanisms of human fungal pathogenesis in Candida albicans relies on reverse genetics approaches, requiring strain engineering. DNA-mediated transformation of C. albicans has been described as highly mutagenic,
Timea Marton +3 more
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CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens
Nucleic Acids Res., 2018 CRISPOR.org is a web tool for genome editing experiments with the CRISPR–Cas9 system. It finds guide RNAs in an input sequence and ranks them according to different scores that evaluate potential off-targets in the genome of interest and predict on ...
J. Concordet, M. Haeussler
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CRISPR/Cas9—Advancing Orthopoxvirus Genome Editing for Vaccine and Vector Development
Viruses, 2018 The clustered regularly interspaced short palindromic repeat (CRISPR)/associated protein 9 (Cas9) technology is revolutionizing genome editing approaches.
Arinze Okoli +3 more
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Molecular Therapy: Nucleic Acids, 2020 Reticuloendotheliosis virus (REV) is an avian retrovirus that causes an oncogenic, immunosuppressive, and runting-stunting syndrome in avian hosts. The co-infection of REV and Marek’s disease virus (MDV), an oncogenic herpesvirus in chickens, further ...
Kai Li +12 more
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CRISPR/Cas9 system: recent applications in immuno-oncology and cancer immunotherapy
Experimental Hematology & Oncology, 2023 Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) is essentially an adaptive immunity weapon in prokaryotes against foreign DNA.
Chen Chen, Zehua Wang, Yanru Qin
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Overview of CRISPR–Cas9 Biology [PDF]
Cold Spring Harbor Protocols, 2016 Prokaryotes use diverse strategies to improve fitness in the face of different environmental threats and stresses, including those posed by mobile genetic elements (e.g., bacteriophages and plasmids). To defend against these elements, many bacteria and archaea use elegant, RNA-directed, nucleic acid–targeting adaptive restriction machineries called ...
Hannah K, Ratner +2 more
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Advancements and Obstacles of CRISPR-Cas9 Technology in Translational Research
Molecular Therapy: Methods & Clinical Development, 2019 The expanding CRISPR-Cas9 technology is an easily accessible, programmable, and precise gene-editing tool with numerous applications, most notably in biomedical research.
Liting You +5 more
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Scientific Reports, 2022 The CRISPR-Cas system is widely used for genome editing of cultured cells and organisms. The discovery of a new single RNA-guided endonuclease, CRISPR-Cas12a, in addition to the conventional CRISPR-Cas9 has broadened the number of editable target sites ...
Riki Ishibashi +4 more
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Frontiers in Marine Science, 2021 The Fujian oyster (Crassostrea angulate) is an important marine bivalve mollusk with high economic value. Gene function research and gene editing techniques have broad application prospects in oyster. The clustered regularly interspaced short palindromic
Kaidi Jin +11 more
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