Results 51 to 60 of about 254,775 (306)

CRISPR/Cas9 Genome Editing Technology: A Valuable Tool for Understanding Plant Cell Wall Biosynthesis and Function

Frontiers in Plant Science, 2020
For the past 5 years, clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) technology has appeared in the molecular biology research spotlight.
Yuan Zhang, Yuan Zhang, Allan M. Showalter, Allan M. Showalter   +3 more
doaj   +1 more source

Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation

Nature Biotechnology, 2014
Components of the prokaryotic clustered, regularly interspaced, short palindromic repeats (CRISPR) loci have recently been repurposed for use in mammalian cells.
John G Doench, E. Hartenian, Daniel B. Graham, Z. Tothova, Mudra Hegde, Ian Smith, Meagan E. Sullender, B. Ebert, R. Xavier, D. Root   +9 more
semanticscholar   +1 more source

CRISPR/Cas9-Based Antiviral Strategy: Current Status and the Potential Challenge

Molecules, 2019
From its unexpected discovery as a bacterial adaptive immune system to its countless applications as one of the most versatile gene-editing tools, the CRISPR/Cas9 system has revolutionized every field of life science.
Choongho Lee
doaj   +1 more source

Allosteric inhibition of CRISPR-Cas9 by bacteriophage-derived peptides

Genome Biology, 2020
Background CRISPR-Cas9 has been developed as a therapeutic agent for various infectious and genetic diseases. In many clinically relevant applications, constitutively active CRISPR-Cas9 is delivered into human cells without a temporal control system ...
Yan-ru Cui, Shao-jie Wang, Jun Chen, Jie Li, Wenzhang Chen, Shuyue Wang, Bing Meng, Wei Zhu, Zhuhong Zhang, Bei Yang, Biao Jiang, Guang Yang, Peixiang Ma, Jia Liu   +13 more
doaj   +1 more source

Rapid Assessment of CRISPR Transfection Efficiency and Enrichment of CRISPR Induced Mutations Using a Dual-Fluorescent Stable Reporter System

Frontiers in Genome Editing, 2022
The nuclease activity of the CRISPR-Cas9 system relies on the delivery of a CRISPR-associated protein 9 (Cas9) and a single guide RNA (sgRNA) against the target gene.
Karim E. Shalaby, Karim E. Shalaby, Mustapha Aouida, Vijay Gupta, Simona S. Ghanem, Omar M. A. El-Agnaf, Omar M. A. El-Agnaf   +6 more
doaj   +1 more source

Nanoparticles for CRISPR–Cas9 delivery [PDF]

Nature Biomedical Engineering, 2017
The DNA mutation that causes Duchenne muscular dystrophy in mice can be corrected, with minimal off-target effects, by gold nanoparticles carrying the CRISPR components.
Zachary, Glass, Yamin, Li, Qiaobing, Xu
openaire   +2 more sources

Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing

Frontiers in Genetics, 2021
The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool for genome manipulation. However, its low intracellular delivery efficiency severely compromises
Li Duan, Li Duan, Li Duan, Kan Ouyang, Xiao Xu, Xiao Xu, Limei Xu, Limei Xu, Caining Wen, Caining Wen, Xiaoying Zhou, Xiaoying Zhou, Zhuan Qin, Zhuan Qin, Zhiyi Xu, Zhiyi Xu, Wei Sun, Yujie Liang   +17 more
doaj   +1 more source

High-capacity adenovector delivery of forced CRISPR-Cas9 heterodimers fosters precise chromosomal deletions in human cells

Molecular Therapy: Nucleic Acids, 2023
Genome editing based on dual CRISPR-Cas9 complexes (multiplexes) permits removing specific genomic sequences in living cells leveraging research on functional genomics and genetic therapies.
Francesca Tasca, Marcella Brescia, Jin Liu, Josephine M. Janssen, Kamel Mamchaoui, Manuel A.F.V. Gonçalves   +5 more
doaj   +1 more source

Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection

Nature, 2017
Chimeric antigen receptors (CARs) are synthetic receptors that redirect and reprogram T cells to mediate tumour rejection. The most successful CARs used to date are those targeting CD19 (ref. 2), which offer the prospect of complete remission in patients
Justin Eyquem, J. Mansilla-Soto, Theodoros Giavridis, Sjoukje J. C. van der Stegen, Mohamad Hamieh, K. Cunanan, Ashlesha Odak, M. Gönen, M. Sadelain   +8 more
semanticscholar   +1 more source

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source