Results 51 to 60 of about 248,807 (309)

Verification of DNA motifs in Arabidopsis using CRISPR/Cas9-mediated mutagenesis. [PDF]

, 2018
Transcription factors (TFs) and chromatin-modifying factors (CMFs) access chromatin by recognizing specific DNA motifs in their target genes. Chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq) has been widely used to discover
Chen, Chen, Chen, Huhui, Chen, Xuemei, Cui, Yuhai, Li, Chenlong, Wang, Suikang   +5 more
core   +2 more sources

Rewriting Human History and Empowering Indigenous Communities with Genome Editing Tools. [PDF]

, 2020
Appropriate empirical-based evidence and detailed theoretical considerations should be used for evolutionary explanations of phenotypic variation observed in the field of human population genetics (especially Indigenous populations). Investigators within
Fox, Keolu, Komor, Alexis C, Rallapalli, Kartik Lakshmi   +2 more
core   +2 more sources

Allosteric inhibition of CRISPR-Cas9 by bacteriophage-derived peptides

Genome Biology, 2020
Background CRISPR-Cas9 has been developed as a therapeutic agent for various infectious and genetic diseases. In many clinically relevant applications, constitutively active CRISPR-Cas9 is delivered into human cells without a temporal control system ...
Yan-ru Cui, Shao-jie Wang, Jun Chen, Jie Li, Wenzhang Chen, Shuyue Wang, Bing Meng, Wei Zhu, Zhuhong Zhang, Bei Yang, Biao Jiang, Guang Yang, Peixiang Ma, Jia Liu   +13 more
doaj   +1 more source

Beyond seek and destroy: How to generate allelic series using genome editing tools [PDF]

, 2020
Genome editing tools have greatly facilitated the functional analysis of genes of interest by targeted mutagenesis. Many usable genome editing tools, including different site-specific nucleases and editor databases that allow single-nucleotide ...
Bes, Martine, Chaine, Christian, Durandet, Franz, Guiderdoni, Emmanuel, Herbert, Leo, Meunier, Anne Cecile, Michel, Rémy, Portefaix, Murielle, Périn, Christophe, This, Patrice, Vernet, Aurore   +10 more
core   +3 more sources

CRISPR/Cas9-Based Antiviral Strategy: Current Status and the Potential Challenge

Molecules, 2019
From its unexpected discovery as a bacterial adaptive immune system to its countless applications as one of the most versatile gene-editing tools, the CRISPR/Cas9 system has revolutionized every field of life science.
Choongho Lee
doaj   +1 more source

Advances in biotechnology: genomics and genome editing [PDF]

, 2017
Genomics, the study of genes, their functions and related techniques has become a crucial science for developing understanding of life processes and how they evolve.
Beccari, Tommaso, Dundar, Munis, Gartland, Jill S., Gartland, Kevan M.A., Magni, Mariapia Viola   +4 more
core   +1 more source

High-capacity adenovector delivery of forced CRISPR-Cas9 heterodimers fosters precise chromosomal deletions in human cells

Molecular Therapy: Nucleic Acids, 2023
Genome editing based on dual CRISPR-Cas9 complexes (multiplexes) permits removing specific genomic sequences in living cells leveraging research on functional genomics and genetic therapies.
Francesca Tasca, Marcella Brescia, Jin Liu, Josephine M. Janssen, Kamel Mamchaoui, Manuel A.F.V. Gonçalves   +5 more
doaj   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

High-Resolution Analysis of the Efficiency, Heritability, and Editing Outcomes of CRISPR/Cas9-Induced Modifications of NCED4 in Lettuce (Lactuca sativa). [PDF]

, 2018
CRISPR/Cas9 is a transformative tool for making targeted genetic alterations. In plants, high mutation efficiencies have been reported in primary transformants. However, many of the mutations analyzed were somatic and therefore not heritable.
Bertier, Lien D, Bradford, Kent J, Britt, Anne B, Huo, Heqiang, Michelmore, Richard W, Ron, Mily   +5 more
core   +2 more sources

Nanoparticle Delivery of CRISPR/Cas9 for Genome Editing

Frontiers in Genetics, 2021
The emerging clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated system (Cas) gene-editing system represents a promising tool for genome manipulation. However, its low intracellular delivery efficiency severely compromises
Li Duan, Li Duan, Li Duan, Kan Ouyang, Xiao Xu, Xiao Xu, Limei Xu, Limei Xu, Caining Wen, Caining Wen, Xiaoying Zhou, Xiaoying Zhou, Zhuan Qin, Zhuan Qin, Zhiyi Xu, Zhiyi Xu, Wei Sun, Yujie Liang   +17 more
doaj   +1 more source