Results 51 to 60 of about 216,936 (304)

CRISPR/Cas9 Genome Editing Technology: A Valuable Tool for Understanding Plant Cell Wall Biosynthesis and Function

Frontiers in Plant Science, 2020
For the past 5 years, clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) technology has appeared in the molecular biology research spotlight.
Yuan Zhang, Yuan Zhang, Allan M. Showalter, Allan M. Showalter   +3 more
doaj   +1 more source

CRISPR/Cas9—Advancing Orthopoxvirus Genome Editing for Vaccine and Vector Development

Viruses, 2018
The clustered regularly interspaced short palindromic repeat (CRISPR)/associated protein 9 (Cas9) technology is revolutionizing genome editing approaches.
Arinze Okoli, Malachy I. Okeke, Morten Tryland, Ugo Moens   +3 more
doaj   +1 more source

Prevention of Avian Retrovirus Infection in Chickens Using CRISPR-Cas9 Delivered by Marek’s Disease Virus

Molecular Therapy: Nucleic Acids, 2020
Reticuloendotheliosis virus (REV) is an avian retrovirus that causes an oncogenic, immunosuppressive, and runting-stunting syndrome in avian hosts. The co-infection of REV and Marek’s disease virus (MDV), an oncogenic herpesvirus in chickens, further ...
Kai Li, Yongzhen Liu, Zengkun Xu, Yu Zhang, Yongxiu Yao, Venugopal Nair, Changjun Liu, Yanping Zhang, Yulong Gao, Xiaole Qi, Hongyu Cui, Li Gao, Xiaomei Wang   +12 more
doaj   +1 more source

Development of an in vivo cleavable donor plasmid for targeted transgene integration by CRISPR-Cas9 and CRISPR-Cas12a

Scientific Reports, 2022
The CRISPR-Cas system is widely used for genome editing of cultured cells and organisms. The discovery of a new single RNA-guided endonuclease, CRISPR-Cas12a, in addition to the conventional CRISPR-Cas9 has broadened the number of editable target sites ...
Riki Ishibashi, Ritsuko Maki, Satsuki Kitano, Hitoshi Miyachi, Fumiko Toyoshima   +4 more
doaj   +1 more source

Rapid Assessment of CRISPR Transfection Efficiency and Enrichment of CRISPR Induced Mutations Using a Dual-Fluorescent Stable Reporter System

Frontiers in Genome Editing, 2022
The nuclease activity of the CRISPR-Cas9 system relies on the delivery of a CRISPR-associated protein 9 (Cas9) and a single guide RNA (sgRNA) against the target gene.
Karim E. Shalaby, Karim E. Shalaby, Mustapha Aouida, Vijay Gupta, Simona S. Ghanem, Omar M. A. El-Agnaf, Omar M. A. El-Agnaf   +6 more
doaj   +1 more source

Nanoparticles for CRISPR–Cas9 delivery [PDF]

Nature Biomedical Engineering, 2017
The DNA mutation that causes Duchenne muscular dystrophy in mice can be corrected, with minimal off-target effects, by gold nanoparticles carrying the CRISPR components.
Zachary, Glass, Yamin, Li, Qiaobing, Xu
openaire   +2 more sources

Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9

, 2014
This work was supported in part by NIH grant 1DP5OD017892 to S.LToxoplasma gondii is a parasite of humans and animals, and a model for other apicomplexans including Plasmodium spp., the causative agents of malaria. Despite many advances, manipulating the
Saima M Sidik, Sebastian Lourido, Caroline G. Hackett (226300), Hackett, Caroline G., Lourido, Sebastian, Caroline G Hackett, Saima M. Sidik (590022), Nicholas J Westwood, Nicholas J. Westwood (254891), Sidik, Saima M., Sebastian Lourido (590023), Fanny Tran, Tran, Fanny, Westwood, Nicholas J., Fanny Tran (571869)   +14 more
core   +1 more source

Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity

, 2015
EC is supported by the Alexander von Humboldt Foundation, the German Federal Ministry for Education and Research, the Helmholtz Association, the Göran Gustafsson Foundation, the Swedish Research Council, the Kempe Foundation and Umeå University.
White, Malcolm F., Charpentier, Emmanuelle, White, Malcolm F, van der Oost, John, Richter, Hagen   +4 more
core   +1 more source

A DNA assembly toolkit to unlock the CRISPR/Cas9 potential for metabolic engineering [PDF]

, 2023
CRISPR/Cas9-based technologies are revolutionising the way we engineer microbial cells. One of the key advantages of CRISPR in strain design is that it enables chromosomal integration of marker-free DNA, eliminating laborious and often inefficient marker
Dmitrii  Bubnov, Rodrigo Ledesma-Amaro, Olga E. Melkina, Melkina, Olga, Yuzbasheva, E. Y., Patel, Davina, Tigran V. Yuzbashev, Dietz, Heiko, Heiko Dietz, Patel, D., Yuzbashev, T., Ledesma-Amaro, R., Melkina, O. E., Bubnov, D., Bubnov, Dmitrii, Evgeniya Y. Yuzbasheva, Davina Patel, Dietz, H., Yuzbashev, Tigran, Yuzbashev, Evgeniya, Ledesma Amaro, Rodrigo   +20 more
core   +1 more source

Cas6 specificity and CRISPR RNA loading in a complex CRISPR-Cas system

, 2014
This research was funded in part by Biotechnology and Biological Sciences Research Council [BB/K000314/1]. The APC was paid through RCUK OA block grant funds.CRISPR-Cas is an adaptive prokaryotic immune system, providing protection against viruses and ...
Malcolm F. White, White, Malcolm F, Shirley Graham, Sokolowski, Richard David, Graham, Shirley, Richard D. Sokolowski   +5 more
core   +1 more source