Results 71 to 80 of about 254,775 (306)

The tsunami named CRISPR/Cas9 [PDF]

Revue Neurologique, 2018
It is rare to witness a breakthrough technology that results in a multidimensional revolution in human health that also has impacts on animals and plants as well as biodiversity and the environment. The tsunami associated with the latest genome editing techniques is most likely not comparable to anything we have known thus far.
openaire   +3 more sources

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

A CRISPR–Cas9 gene drive targeting doublesex causes complete population suppression in caged Anopheles gambiae mosquitoes

Nature Biotechnology, 2018
In the human malaria vector Anopheles gambiae, the gene doublesex (Agdsx) encodes two alternatively spliced transcripts, dsx-female (AgdsxF) and dsx-male (AgdsxM), that control differentiation of the two sexes.
Kyros Kyrou, Andrew M. Hammond, R. Galizi, Nace Kranjc, A. Burt, Andrea K. Beaghton, T. Nolan, A. Crisanti   +7 more
semanticscholar   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression

Plant Methods, 2018
Background When developing CRISPR/Cas9 systems for crops, it is crucial to invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes, especially if the transformation system is difficult or time-consuming.
Lu Long, Dan-Dan Guo, Wei Gao, Wen-Wen Yang, Li-Pan Hou, Xiao-Nan Ma, Yu-Chen Miao, Jose Ramon Botella, Chun-Peng Song   +8 more
doaj   +1 more source

In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration

Nature, 2016
Targeted genome editing via engineered nucleases is an exciting area of biomedical research and holds potential for clinical applications. Despite rapid advances in the field, in vivo targeted transgene integration is still infeasible because current ...
Keiichiro Suzuki, Yuji Tsunekawa, R. Hernández-Benítez, Jun Wu, Jie Zhu, Euiseok J. Kim, Fumiyuki Hatanaka, Mako Yamamoto, T. Araoka, Zhe Li, M. Kurita, Tomoaki Hishida, Mo Li, Emi Aizawa, Shicheng Guo, S. Chen, April Goebl, R. Soligalla, J. Qu, Tingshuai Jiang, Xin-Yuan Fu, Maryam Jafari, C. R. Esteban, W. Berggren, J. Lajara, E. Núñez-Delicado, Pedro Guillén, J. Campistol, F. Matsuzaki, Guang-Hui Liu, P. Magistretti, Kun Zhang, E. Callaway, Kang Zhang, J. Belmonte   +34 more
semanticscholar   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Enhanced proofreading governs CRISPR-Cas9 targeting accuracy

Nature, 2017
The RNA-guided CRISPR–Cas9 nuclease from Streptococcus pyogenes (SpCas9) has been widely repurposed for genome editing. High-fidelity (SpCas9-HF1) and enhanced specificity (eSpCas9(1.1)) variants exhibit substantially reduced off-target cleavage in human
Janice S. Chen, Y. S. Dagdas, B. Kleinstiver, Moira M. Welch, Alexander Sousa, Lucas B. Harrington, S. H. Sternberg, J. Joung, A. Yildiz, J. Doudna   +9 more
semanticscholar   +1 more source

Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining

Molecular Oncology, EarlyView.
IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis, Jack V. Mills, Wojciech Niedzwiedz, Valentine M. Macaulay   +3 more
wiley   +1 more source

Nanotechnology‐based CRISPR/Cas9 delivery system for genome editing in cancer treatment

MedComm – Biomaterials and Applications
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9 (CRISPR/Cas9) systems initiate a revolution in genome editing, which have a significant potential for treating cancer. A significant amount of research has
Shiyao Zhou, Yingjie Li, Qinjie Wu, Changyang Gong   +3 more
doaj   +1 more source