Results 81 to 90 of about 243,751 (303)

Defining the Target DNA Cut in CRISPR-Cas9

, 2022
Structures of reactants, products and intermediate states during DNA cleavage in the CRISPR-Cas9 HNH nuclease. Data are provided in PDB format. Two VMD states are also provided to facilitate visualization through the Visual Molecular Dynamics (VMD ...
Mohd Ahsan (7301282), Kyle W. East (8275104), Giulia Palermo (12073211), Jonas Binz (12073327), Martin Jinek (7351819), Pablo R. Arantes (8107538), George P. Lisi (2034577), Rohaine V. Hsu (8536737), Łukasz Nierzwicki (1353969), Martin Pacesa (4377673)   +9 more
core   +1 more source

Single‐cell DNA methylation profiling: Technologies, computation, and applications in precision oncology

Molecular Oncology, EarlyView.
Single‐cell DNA methylation (scDNAme) profiling maps epimutational clonal evolution, revealing mechanisms of malignancy and therapeutic resistance across diverse cancer types. By providing a high‐resolution landscape of intratumoral heterogeneity, these technologies empower precise patient stratification, guide the development of enhanced ...
Ik Soo Kim
wiley   +1 more source

A Single H1 Promoter Can Drive Both Guide RNA and Endonuclease Expression in the CRISPR-Cas9 System

Molecular Therapy: Nucleic Acids, 2019
The RNA-guided endonuclease Cas9 (CRISPR-Cas9) genome editing system has been widely used for biomedical research and holds great potential for therapeutic applications in eukaryotes. The conventional vector-based CRISPR-Cas9 delivery system requires two
Zongliang Gao, Elena Herrera-Carrillo, Ben Berkhout   +2 more
doaj   +1 more source

Targeting transcription factors associated with hemoglobinopathies: Lessons from successful interventions and implications for cancer

Molecular Oncology, EarlyView.
This review summarizes the transcription factors, repressive chromatin‐modifying complexes, and epigenetic mechanisms that control fetal hemoglobin repression. Notably, many regulators of γ‐globin silencing also function in transcriptional and epigenetic networks that drive cancer, highlighting opportunities to translate advances in hemoglobinopathy ...
Meigen Yu, Puspa Das, Xing Zhang, Xiaodong Cheng   +3 more
wiley   +1 more source

Simultaneous CRISPR/Cas9-induced double-strand breaks are lethal in models of pancreatic cancer

The Journal of Clinical Investigation
While radiation is an effective oncologic therapy, killing cancer by inducing DNA double-strand breaks (DSBs), it lacks specificity for neoplastic cells.
Selina Shiqing K. Teh, Akhil Kotwal, Alexis Bennett, Eitan Halper-Stromberg, Laura Morsberger, Saum Zamani, Yanan Shi, Alyza Skaist, Qingfeng Zhu, Kirsten Bowland, Hong Liang, Ralph H. Hruban, Chien-Fu Hung, Robert A. Anders, Nicholas J. Roberts, Robert B. Scharpf, Michael Goldstein, Ying S. Zou, James R. Eshleman   +18 more
doaj   +1 more source

CEACAM1 participation in breast cancer progression

Molecular Oncology, EarlyView.
In invasive breast cancer (BC), CEACAM1 shifts from an apical to a uniform membranous/cytoplasmic pattern, or is lost, as tumors dedifferentiate, inversely tracking the Ki‐67 proliferative index. In MCF‐7 cells, only CEACAM1‐4L suppresses proliferation, repressing cell cycle and growth factor genes.
Mykola Lyndin, Irina Kube‐Golovin, Anatolii Romaniuk, Gunther Wennemuth   +3 more
wiley   +1 more source

Application and perspective of CRISPR/Cas9 genome editing technology in human diseases modeling and gene therapy

Frontiers in Genetics
The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) mediated Cas9 nuclease system has been extensively used for genome editing and gene modification in eukaryotic cells.
Man-Ling Zhang, Man-Ling Zhang, Hong-Bin Li, Hong-Bin Li, Yong Jin, Yong Jin   +5 more
doaj   +1 more source

Somatostatin receptor 4 (SSTR4) is a tumor suppressor in cutaneous and head & neck squamous cell carcinomas

Molecular Oncology, EarlyView.
This study identifies somatostatin receptor 4 (Sstr4) as a critical tumor suppressor against skin and head/neck cancers (HNSCC, cSCC, and BCC). The loss of Sstr4 removes a check on cell growth, causing hyperactivation of the MAPK‐ERK signaling pathway (↑).
Ali Taqvi, Tuan Khang Huynh, Adway Kadam, Zahra Kardan, Yue Yan, Amina Jamal Laham, Sampath Kumar Loganathan   +6 more
wiley   +1 more source

CRISPR RNA biogenesis by a Cas6 nuclease [PDF]

, 2015
Clustered regularly interspaced short palindromic repeats (CRISPRs) and associated (Cas) proteins form the basis of a prokaryotic adaptive immune system.
Sokolowski, Richard D.
core   +2 more sources

Comparing successful gene knock-in efficiencies of CRISPR/Cas9 with ZFNs and TALENs gene editing systems in bovine and dairy goat fetal fibroblasts

Journal of Integrative Agriculture, 2018
This study aimed to compare the efficiencies of clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas9-mediated gene knock-ins with zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) in bovine and ...
Hui LIU, Chang LIU, Yu-hang ZHAO, Xue-jie HAN, Zheng-wei ZHOU, Chen WANG, Rong-feng LI, Xue-ling LI   +7 more
doaj   +1 more source