Results 81 to 90 of about 216,936 (304)
Target specificity of the CRISPR‐Cas9 system [PDF]
Quantitative Biology, 2014 The CRISPR‐Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA‐guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases.
Xuebing Wu +2 more
openaire +4 more sources
Molecular Oncology, EarlyView.Beyond its role in immune evasion, this study identified that CD47 drives tumor‐intrinsic signaling in non‐small cell lung cancer (NSCLC). Transcriptomic profiling and functional studies revealed that CD47 regulates cell adhesion, migration, and metastasis through an ERK–EMT signaling axis.
Asa P.Y. Lau +8 more
wiley +1 more source
Molecular Oncology, EarlyView.EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain +10 more
wiley +1 more source
Defining the Target DNA Cut in CRISPR-Cas9
, 2022 Structures of reactants, products and intermediate states during DNA cleavage in the CRISPR-Cas9 HNH nuclease. Data are provided in PDB format. Two VMD states are also provided to facilitate visualization through the Visual Molecular Dynamics (VMD ...
Mohd Ahsan (7301282) +9 more
core +1 more source
Molecular Oncology, EarlyView.Here, we demonstrate that HS1BP3 interacts with Cortactin through a proline‐rich region (PRR3.1) and show that this interaction, and HS1BP3 itself, promote cancer cell proliferation and invasion. Inhibition of this interaction leads to build‐up of TKS5 in multivesicular endosomes and altered secretion of CD63 and CD9, providing an explanation for the ...
Arja Arnesen Løchen +9 more
wiley +1 more source
CRISPR/Cas9 for Insect Pests Management: A Comprehensive Review of Advances and Applications
Agriculture, 2022 Insect pests impose a serious threat to agricultural productivity. Initially, for pest management, several breeding approaches were applied which have now been gradually replaced by genome editing (GE) strategies as they are more efficient and less ...
Sanchita Singh +6 more
doaj +1 more source
Molecular Oncology, EarlyView.Ixazomib inhibits proteasome‐mediated degradation of topoisomerase I induced by irinotecan, thereby restoring drug sensitivity and promoting tumor cell death in colorectal cancer. Irinotecan, a topoisomerase I (topoI) inhibitor, is widely used for colorectal cancer, but resistance remains a major clinical challenge.
Yuho Ebata +10 more
wiley +1 more source
Nanotechnology‐based CRISPR/Cas9 delivery system for genome editing in cancer treatment
MedComm – Biomaterials and ApplicationsThe clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9 (CRISPR/Cas9) systems initiate a revolution in genome editing, which have a significant potential for treating cancer. A significant amount of research has
Shiyao Zhou +3 more
doaj +1 more source
Loss of IGF‐1R impairs DNA‐PKcs recruitment to chromatin leading to defective end‐joining
Molecular Oncology, EarlyView.IGF‐1R promotes radioresistance by facilitating DNA‐PKcs recruitment to chromatin, enabling non‐homologous end‐joining (NHEJ) repair of double‐strand breaks. Inhibition or loss of IGF‐1R disrupts this recruitment to damage sites, driving compensatory reliance on microhomology‐mediated end‐joining (MMEJ) repair.
Matthew O. Ellis +3 more
wiley +1 more source
, 2023 Soil contamination by toxic metals is a major health issue that could be partly solved by using genetically-modified plants. For that, the recently developed technique of clustered regularly interspaced short palindromic repeats (CRISPR) has created a ...
Ghorai, Mimosa +9 more
core +1 more source