Results 91 to 100 of about 243,751 (303)
Molecular Oncology, EarlyView.Taxanes are widely used chemotherapeutics whose effects on cellular mechanics remain poorly understood. We show that paclitaxel induces rapid cellular contraction by promoting GEF‐H1 dissociation from microtubules and non‐muscle myosin II activation through RhoA/ROCK.
Gloria Asensio‐Juárez +5 more
wiley +1 more source
Molecular Oncology, EarlyView.This study identifies ARHGAP5, in addition to the frequently mutated ARHGAP35, as significantly mutated in endometrial cancer. Mutations in both genes co‐occur and are associated with their correlated downregulation. Functional CRISPR studies show that both paralogs regulate similar pathways, including actin cytoskeleton organization.
Mathilde Pinault +12 more
wiley +1 more source
CRISPR/Cas9 as a Mutagenic Factor
International Journal of Molecular SciencesThe discovery of the CRISPR/Cas9 microbial adaptive immune system has revolutionized the field of genetics, by greatly enhancing the capacity for genome editing. CRISPR/Cas9-based editing starts with DNA breaks (or other lesions) predominantly at target sites and, unfortunately, at off-target genome sites.
Andrey R. Shumega +5 more
openaire +2 more sources
The tsunami named CRISPR/Cas9 [PDF]
Revue Neurologique, 2018 It is rare to witness a breakthrough technology that results in a multidimensional revolution in human health that also has impacts on animals and plants as well as biodiversity and the environment. The tsunami associated with the latest genome editing techniques is most likely not comparable to anything we have known thus far.
openaire +3 more sources
FEBS Open Bio, EarlyView.RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Protein Inhibitors of CRISPR-Cas9 [PDF]
ACS Chemical Biology, 2018 Bacteria are under constant predation from viruses, called bacteriophages (phages). This threat has driven the evolution of multiple defense systems, including the CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR associated genes) immune pathway. Phages are not passive bystanders in their CRISPR-mediated demise, however,
openaire +2 more sources
, 2020 Szakdolgozatom során a CRISPR/Cas9 rendszert vizsgáltam, mely egy széleskörűen alkalmazott genomszerkesztő eljárás. Az eljárás előnyének számít az egyszerű és gyors, valamint korlátlan alkalmazhatósága, azonban fontos korlátozója az offtarget hatás, azaz
Mester, Julianna
core
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
CRISPR/Cas9: Prospects and Challenges [PDF]
Human Gene Therapy, 2015 All science takes inspiration from nature, but nowhere is this more true than in biology, where some of the most powerful tools available to researchers are derived from natural products. From restriction enzymes and fluorescent proteins to microbial opsins and viral gene delivery vectors, researchers have capitalized on processes that occur in a ...
openaire +2 more sources
FEBS Open Bio, EarlyView.KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura +19 more
wiley +1 more source