Results 101 to 110 of about 243,751 (303)

Phytoremediation and sequestration of soil metals using the CRISPR/Cas9 technology to modify plants: a review

, 2023
Soil contamination by toxic metals is a major health issue that could be partly solved by using genetically-modified plants. For that, the recently developed technique of clustered regularly interspaced short palindromic repeats (CRISPR) has created a ...
Ghorai, Mimosa, Rajapaksha, Anushka Upamali, Kumar, Ravi, Dey, Abhijit, Aftab, Tariq, Bundschuh, Jochen, Bhattacharyya, Nirjhar, Jha, Niraj Kumar, Bontempi, Elza, Anand, Uttpal   +9 more
core   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

CRISPR/Cas9 delivery with one single adenoviral vector devoid of all viral genes

Scientific Reports, 2017
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system revolutionized the field of gene editing but viral delivery of the CRISPR/Cas9 system has not been fully explored.
Eric Ehrke-Schulz, Maren Schiwon, Theo Leitner, Stephan Dávid, Thorsten Bergmann, Jing Liu, Anja Ehrhardt   +6 more
doaj   +1 more source

Structure of the archaeal Cascade subunit Csa5 : Relating the small subunits of CRISPR effector complexes [PDF]

, 2013
This work was funded by a grant from the Biotechnology and Biological Sciences Research Council (BBSRC) (REF: BB/G011400/1) to M.F.W. and J.H.N. and a BBSRC-funded studentship to J.R.The Cascade complex for CRISPR-mediated antiviral immunity uses CRISPR ...
Naismith, Jim, White, Malcolm F, Anderson, Linzi, Liu, Huanting, Reeks, Judith Anne, Graham, Shirley   +5 more
core   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

CRISPR/Cas9

Drug Discovery Today, 2019
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated nuclease 9 (Cas9), as a powerful genome-editing tool, has revolutionized genetic engineering. It is widely used to investigate the molecular basis of different cancer types. In this review, we present an overview of recent studies in which CRISPR/Cas9 has been used for
Liu, Bin, Saber, Ali, Haisma, Hidde J
openaire   +1 more source

CRISPR/Cas9 and Genome Editing in Drosophila

Journal of Genetics and Genomics, 2014
Recent advances in our ability to design DNA binding factors with specificity for desired sequences have resulted in a revolution in genetic engineering, enabling directed changes to the genome to be made relatively easily. Traditional techniques for generating genetic mutations in most organisms have relied on selection from large pools of randomly ...
Bassett, Andrew R., Liu, Ji-Long
openaire   +3 more sources

CRISPR-Cas9 for in vivo Gene Therapy: Promise and Hurdles

Molecular Therapy: Nucleic Acids, 2016
Owing to its easy-to-use and multiplexing nature, the genome editing tool CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats (CRISPR) associated nuclease 9) is revolutionizing many areas of medical research and one of the most amazing
Wei-Jing Dai, Li-Yao Zhu, Zhong-Yi Yan, Yong Xu, Qi-Long Wang, Xiao-Jie Lu   +5 more
doaj   +1 more source

Cell surface CD11c as a neutrophil aging marker molecule

FEBS Open Bio, EarlyView.
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki, Fahd Alhamdan, Erik Malm, Marie Bermudez, Samuel Y. Kim, Koichi Yuki   +5 more
wiley   +1 more source