Results 121 to 130 of about 243,751 (303)
Advanced Functional Materials, EarlyView.Schematic illustration of LNP‐MPG nuclei‐targeting delivery of HMW‐FGF2 promoting histone acetylation to regulate the fate of DPSCs and treat spinal cord injury. LNPs components include pHMW‐FGF2 plasmid, DSPC, Dlin‐MC3‐DMA, cholesterol, and PEG2000, and are modified with MPG to form HMW‐FGF2@LNP‐MPG (HLM). HLM nuclei‐targets DPSCs to deliver HMW‐FGF2,
Heng Zhou +6 more
wiley +1 more source
Genomszerkesztés a CRISPR/Cas9 rendszerrel
, 2016 A legújabb genomszerkesztési technika az úgynevezett CRISPR/Cas9 rendszer. Ez nem egy ember által kreált, mesterséges szerkezet, a baktériumok használják a vírus fertőzések ellen, mint egy adaptív immunrendszert.
Kovács, Balázs Lajos
core
Advanced Healthcare Materials, EarlyView.Engineered red blood cell‐derived extracellular vesicles (eRBCEVs) are synthesized via controlled microfluidic assembly from native RBC lipids, enabling tunable encapsulation of proteins, nucleic acids, nanoparticles, and viral vectors. The platform demonstrates reproducible nanoscale architecture, preserved membrane composition, and functional cargo ...
Chiranth K. Nagaraj +23 more
wiley +1 more source
, 2023 In CRISPR/Cas9 system, there are two critical parts: sgRNA sequence and Cas9 sequence. These two sequence could be cloned into separated plasmids or combined into one plasmids. Considering the limited utility of protoplast system, it’s also available to transfect preassembled complexes of purified Cas9 protein and synthesized sgRNA.
openaire +2 more sources
Advanced Healthcare Materials, EarlyView.Mitochondria‐targeted nanotherapies emerge as a promising strategy for combating aging‐associated neurodegenerative disorders (NDs) by restoring mitochondrial function, reducing oxidative stress, and improving neuronal survival. Recent advances in nanotechnology, therapeutic delivery, and translational research are highlighted, providing insights into ...
Dnyandev G. Gadhave +8 more
wiley +1 more source
Использование технологии CRISPR/Cas9 для получения мутантов по генам транспортеров серотонина у Danio rerio [PDF]
, 2018 Технология CRISPR/Cas9 – новейший метод редактирования геномов, основанный на адаптивном иммунитете прокариот. Для её применения необходим особый РНК-гид, направляющий эффекторную нуклеазу этой системы к специфической мишени в геноме, и сама нуклеаза ...
Кисель Элана Вадимовна +1 more
core
Advanced Healthcare Materials, EarlyView.Biologically derived and hybrid nonviral nanovectors are examined as distinct but convergent design approaches. Integrating synthetic components with biologically functionalized membranes allows efficient interactions with complex cellular environments.
Clara Baldari +10 more
wiley +1 more source
Advanced Healthcare Materials, EarlyView.FeS‐VA@lanifibranor (FVL), an activated hepatic stellate cell (aHSC)‐targeted nanoplatform comprising vitamin A‐functionalized two‐dimensional iron sulfide nanosheets and lanifibranor, is engineered for precision therapy of liver fibrosis. FVL suppresses the TGF‐β signaling pathway by inhibiting SMAD2/3 phosphorylation and induces ferroptosis in aHSCs,
Yinuo Yang +11 more
wiley +1 more source
Transient Cytoskeletal Anisotropy Encodes Short‐Term Mechanical Memory in Glioblastoma Cells
Advanced Materials, EarlyView.The same mechanical deformation can produce distinct cytoskeletal states depending on loading history. Experiments and constitutive modeling reveal that transient actin–vimentin anisotropy stores mechanical information and governs short‐term mechanical memory in glioblastoma cells.
Clara Gomez‐Cruz +5 more
wiley +1 more source
Secondary Conformational Checkpoint in CRISPR-Cas9
A specific checkpoint between target DNA binding and cleavage primarily governs the precision of Cas9 gene editing. Although various CRISPR-Cas9 variants have been developed to improve DNA cleavage accuracy, we still lack a comprehensive understanding of
Shuxin Zhao (18387413) +2 more
core +2 more sources