Results 141 to 150 of about 216,936 (304)
, 2020 A Coleophoma empetri szulfotranszferáz gén azonosítása fontos tényező lehet a micafungin és más antifungális hatóanyagok vízoldékonyságának növelésében. Ehhez egy S.
Varga, Kinga Edina
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Advanced Science, EarlyView.Pancreatic neuroendocrine tumors frequently silence MEN1 through epigenetic mechanisms. Here, SIRT7 recruits DNMT1 to the MEN1 promoter, drives hypermethylation, and enhances DNA repair. Inhibiting SIRT7 restores MEN1, reduces MRN complex abundance, impairs double‐strand break repair, and sensitizes PanNET models to radiation, supporting SIRT7 as a ...
Jianyun Jiang +11 more
wiley +1 more source
Introduction to CRISPR-Cas9 Techniques
This open-access textbook provides an in-depth introduction into the CRISPR-cas9 technology and explores its use across the gamut of biological model systems.
core +1 more source
, 2023 In CRISPR/Cas9 system, there are two critical parts: sgRNA sequence and Cas9 sequence. These two sequence could be cloned into separated plasmids or combined into one plasmids. Considering the limited utility of protoplast system, it’s also available to transfect preassembled complexes of purified Cas9 protein and synthesized sgRNA.
openaire +2 more sources
G3BP1 Succinylation at K413 is Critical for Cardiac Function by Modulating PI3K‐AKT‐mTOR Signal Axis
Advanced Science, EarlyView.Schematic illustrating the impact of G3BP1 succinylation at K413 on cardiac function. In the healthy human heart, G3BP1 succinylation maintains homeostatic mTOR signaling. In patients with dilated cardiomyopathy (DCM) and heart failure (HF), G3BP1 de‐succinylation induces RagA expression and disrupts the binding of the TSC1/2 complex, leading to the ...
Yuan Zhang +9 more
wiley +1 more source
Advanced Science, EarlyView.A comprehensive meta‐analysis of expression quantitative trait loci (eQTLs) across five diverse tomato populations reveals a high‐resolution atlas of transcriptional regulation and uncovers conserved and population‐specific regulatory architectures underlying fruit nutritional quality traits, including flavonoids, sugars, organic acids, carotenoids ...
Jiantao Zhao +14 more
wiley +1 more source
Highly Effective and Low-Cost MicroRNA Detection with CRISPR-Cas9
, 2018 MicroRNAs have been reported as related to multiple diseases and have potential applications in diagnosis and therapeutics. However, detection of miRNAs remains improvable, given their complexity, high cost, and low sensitivity as of currently.
Lingyun Zhu (35902) +10 more
core +1 more source
MoleculesGenome editing is a promising therapeutic strategy for genetic disorders by modifying the genome precisely, especially the CRISPR/Cas9 system. However, a major limitation of CRISPR/Cas9 in gene therapy is the biosafety issues caused by off-target effects.
Jiasong Chang +9 more
doaj +1 more source
Advanced Science, EarlyView.Lactate in cervical cancer induces HNRNPU K181 lactylation, opposed by NAA50‐mediated acetylation and suppressed by Pazopanib. This lactylation enhances HNRNPU binding to PHGDH pre‐mRNA exon 1, maintaining exon 1‐containing transcripts and mRNA stability, thereby activating serine metabolism.
Chang Zhang +6 more
wiley +1 more source
A CRISPR technológia alkalmazása Aspergillus terreusban
, 2017 The Aspergillus terreus NRRL1960 is a filamentous fungus and strong itaconic acid producer. There are no genetic markers and tools available for this strain, and in this way it is hard to engineer.
Almási, Réka
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