Results 161 to 170 of about 216,936 (304)

Harnessing noncanonical crRNA for highly efficient genome editing

Nature Communications
The CRISPR-Cas12a system is more advantageous than the widely used CRISPR-Cas9 system in terms of specificity and multiplexibility. However, its on-target editing efficiency is typically much lower than that of the CRISPR-Cas9 system.
Guanhua Xun, Zhixin Zhu, Nilmani Singh, Jingxia Lu, Piyush K. Jain, Huimin Zhao   +5 more
doaj   +1 more source

CRISPR-Cas9 as a Trojan horse

Molecular Therapy, 2023
openaire   +2 more sources

Cuproptosis and Mitophagy Mediated by the THUMPD1/IGF2R‐Dependent Suppression of AKT and Activation of AMPK Signaling Suppress Lung Adenocarcinoma Progression

Advanced Science, EarlyView.
THUMPD1 drives a tumor‐suppressive signaling cascade in lung adenocarcinoma by promoting IGF2R expression. IGF2R associates with PPP2R1A to suppress AKT and activate AMPK, leading to SLC31A1 upregulation and copper accumulation. Elevated copper disrupts mitochondrial metabolism and induces excessive mitophagy, thereby restraining tumor growth and ...
Kai Wu, Bo Qin, Zhuoyu Gu, Weizheng Ding, Xiaoming Chen, Kaishang Zhang, Yujin Qiao, Shuang Yuan, Song Zhao, Xiangnan Li, Peng Zhang   +10 more
wiley   +1 more source

Recent Advancements in Reducing the Off-Target Effect of CRISPR-Cas9 Genome Editing

Misganaw Asmamaw Mengstie,1 Muluken Teshome Azezew,2 Tadesse Asmamaw Dejenie,3 Assefa Agegnehu Teshome,4 Fitalew Tadele Admasu,1 Awgichew Behaile Teklemariam,1 Anemut Tilahun Mulu,1 Melaku Mekonnen Agidew,1 Dagnew Getnet Adugna,5 Habtamu Geremew,6 ...
Tadele Admasu F, Tilahun Mulu A, Teshome Azezew M, Mekonnen Agidew M, Adugna DG, Geremew H, Behaile Teklemariam A, Asmamaw Dejenie T, Abebe EC, Asmamaw Mengstie M, Teshome AA   +10 more
core  

UCHL3 Regulates Subgenomic Flaviviral RNA Condensates to Promote Virus Propagation

Advanced Science, EarlyView.
ABSTRACT Flavivirus subgenomic RNAs (sfRNAs) antagonise antiviral defences, yet how sfRNAs are organized and maintained in cells remains poorly understood. Here we identify ubiquitin C‐terminal hydrolase L3 (UCHL3) as a post‐translational regulator of flavivirus sfRNA stability and function.
Oscar Trejo‐Cerro, Anna Beekmayer‐Dhillon, Qi Wen Teo, Lewis Siu, Ming Yuan Li, Kate J. Heesom, Adán Pinto‐Fernández, Sumana Sanyal   +7 more
wiley   +1 more source

Applications of CRISPR/Cas9 technology for tissue-specific genes inactivation in zebrafish embryos: a review of the methodology.

reservedLo sviluppo della tecnologia CRISPR/Cas9 per l’editing del genoma da parte di Charpentier e Doudna ha rivoluzionato il modo di fare ricerca. Questa tecnica, che ha valso loro il premio Nobel per la chimica nel 2020, si basa sull’utilizzo di una ...
MARCON, ELISA
core  

CRISPR-Cas9 knockout of CLU does not affect WPB number or size.

A CLUKO HUVEC cell line generated via CRISPR-Cas9 was analyzed for WPB number and WPB size. Representative immunofluorescent images showing DAPI (blue) and VWF staining (green) (5A) demonstrates appropriate cellular confluence and the expected shape of ...
Allaura A. Cox (17980027), Alice Liu (4814415), Christopher J. Ng (14290358)   +2 more
core   +1 more source

Targeting Supramolecular Active Complexes of Nav1.7/Nav1.8 to Relieve Chronic Neuropathic Pain

Advanced Science, EarlyView.
In mice and patients with severe chronic neuropathic pain (NP), Nav1.7, Nav1.8, TrkB, and five cytoskeletal proteins form supramolecular active complexes (SMACs) with polygonal lattice structures as noxious signal amplifiers in dorsal root ganglion (DRG) neurons.
Liting Sun, Hang Xian, Yunxin Shi, Taotan Yang, Hongyan Shuai, Wenchao Hu, Siying Fei, Miao Xu, Taoyuan Yang, Ruilong Xia, Ting Wen, Fengting Zhu, Yan Fu, Yang Li, Wei Xia, Ran Qian, Yuanying Liu, Zhicheng Tian, Lamei Li, Qian Zhou, Lize Xiong, Rui Cong, Ceng Luo, Shengxi Wu, Xiafeng Shen, Xin Yu, Rou‐Gang Xie, Changgeng Peng   +27 more
wiley   +1 more source

Strategies and Applications of Genomic Editing in Plants with CRISPR/Cas9

CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9) has revolutionized the field of genome editing, providing a powerful and versatile tool for precise modification of genetic material.
Ghorai, Mimosa, Mohiuddin, Tabasum, Bhattacharya, Sayan, Nongdam, Potshangbam, Al-Tawaha, Abdel Rahman, Mandal, Sayanti, Hurrah, Ishfaq Majid   +6 more
core   +1 more source

Engineering Approaches to Modify Immunomodulatory Functions of Mesenchymal Stromal Cells (MSCs): Tissue Regeneration and Clinical Application

Advanced Science, EarlyView.
Mesenchymal stromal cells (MSCs) show promise for treating immune‐related disorders through immunomodulation and tissue regeneration. This review gives a brief overview of current clinical approval of MSC therapies. It also discussed how bioengineering, including genetic modification, biomaterial delivery, extracellular vesicles, and iPSC‐derived MSCs,
Sichen Yang, Kejia Li, Ning Wang, Zhen Li, Zhiyong Zhang, Rocky S. Tuan, Yangzi Jiang   +6 more
wiley   +1 more source