Results 61 to 70 of about 254,775 (306)

Precision genome editing in plants: state-of-the-art in CRISPR/Cas9-based genome engineering

BMC Plant Biology, 2020
Traditionally, generation of new plants with improved or desirable features has relied on laborious and time-consuming breeding techniques. Genome-editing technologies have led to a new era of genome engineering, enabling an effective, precise, and rapid
Naoki Wada, Risa Ueta, Yuriko Osakabe, Keishi Osakabe   +3 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Epigenome editing by a CRISPR/Cas9-based acetyltransferase activates genes from promoters and enhancers

Nature Biotechnology, 2015
Technologies that enable targeted manipulation of epigenetic marks could be used to precisely control cell phenotype or interrogate the relationship between the epigenome and transcriptional control.
Isaac B. Hilton, A. D'Ippolito, Christopher M. Vockley, Pratiksha I. Thakore, G. Crawford, Timothy E. Reddy, C. Gersbach   +6 more
semanticscholar   +1 more source

CRISPR/Cas9 therapeutics: progress and prospects

Signal Transduction and Targeted Therapy, 2023
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene-editing technology is the ideal tool of the future for treating diseases by permanently correcting deleterious base mutations or disrupting disease-
Tianxiang Li, Yanyan Yang, Hongzhao Qi, Weigang Cui, Lin Zhang, Xiuxiu Fu, Xiangqin He, Meixin Liu, Pei-feng Li, Tao Yu   +9 more
semanticscholar   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

High-fidelity CRISPR-Cas9 variants with undetectable genome-wide off-targets

Nature, 2016
CRISPR–Cas9 nucleases are widely used for genome editing but can induce unwanted off-target mutations. Existing strategies for reducing genome-wide off-target effects of the widely used Streptococcus pyogenes Cas9 (SpCas9) are imperfect, possessing only ...
B. Kleinstiver, V. Pattanayak, Michelle S. Prew, S. Tsai, Nhu T. Nguyen, Zongli Zheng, J. Joung   +6 more
semanticscholar   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Genome-scale CRISPR-Cas9 Knockout and Transcriptional Activation Screening

Nature Protocols, 2017
Forward genetic screens are powerful tools for the unbiased discovery and functional characterization of specific genetic elements associated with a phenotype of interest.
J. Joung, S. Konermann, J. Gootenberg, O. Abudayyeh, Randall J. Platt, Mark D. Brigham, Neville E. Sanjana, Feng Zhang   +7 more
semanticscholar   +1 more source

AscF in the mycobacterial CIII–CIV supercomplex lacks metal and nucleotide binding but links malate oxidation to respiration

FEBS Letters, EarlyView.
The mycobacterial CIII‐CIV respiratory supercomplex is an obligate assembly, encompassing several subunits of unknown functions. We have characterized the intracellular subunit AscF, and show that it is unlikely to be a sensor for metals or nucleotides, but is required for growth on nonfermentable energy sources, and likely works as an adapter for ...
Eni Rile, Riccardo Diamanti, Daniel Lundin, Soni Sharma, Robin Lissner, Coenraad P. Kuil, Martin Högbom, Dirk Bald, Dan Sjöstrand   +8 more
wiley   +1 more source

CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling

Cell, 2014
SUMMARY CRISPR-Cas9 is a versatile genome editing technology for studying the function of genetic elements. To broadly enable the application of Cas9 in vivo, we established a Cre-dependent Cas9 knockin mouse.
Randall J. Platt, Sidi Chen, Yang Zhou, Michael J. Yim, L. Swiech, Hannah R. Kempton, J. Dahlman, Oren Parnas, T. Eisenhaure, M. Jovanović, Daniel B. Graham, S. Jhunjhunwala, R. Xavier, R. Langer, Daniel G. Anderson, N. Hacohen, A. Regev, G. Feng, P. Sharp, Feng Zhang   +19 more
semanticscholar   +1 more source