Results 11 to 20 of about 93,364 (279)
Prime Editing in Dividing and Quiescent Cells. [PDF]
Int J Mol SciPrime editing is a method of genome editing based on reverse transcription. Recent results have shown its elevated efficiency in dividing cells, which raises some questions regarding the mechanism of this effect, because prime editing does not employ homology-driven repair.
Petrova IO, Smirnikhina SA.
europepmc +4 more sources
Base editing and prime editing in laboratory animals [PDF]
Laboratory Animals, 2021 Genome editing by programmable RNA-dependent Cas endonucleases has revolutionised the field of genome engineering, achieving targeted genomic change at unprecedented efficiencies with considerable application in laboratory animal research. Despite its ease of use and wide application, there remain concerns about the precision of this technology and a ...
Federico Caso, Benjamin Davies
core +6 more sources
Development and Application of Prime Editing in Plants
Rice Science, 2023 Clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein (Cas)-mediated genome editing has greatly accelerated progress in plant genetic research and agricultural breeding by enabling targeted genomic modifications. Moreover,
Liu Tingting +5 more
doaj +2 more sources
Explore the dominant factor in prime editing via a view of DNA processing
Synthetic and Systems Biotechnology, 2023 Prime editing is a revolutionary gene-editing method that is capable of introducing insertions, deletions and base substitutions into the genome. However, the editing efficiency of Prime Editor (PE) is limited by the DNA repair process.
Zhimeng Xu +6 more
doaj +2 more sources
Mutation-specific reporter for optimization and enrichment of prime editing [PDF]
Nature Communications, 2022 While prime editing is a promising technique, some genomic sites remain difficult to edit. Here the authors present fluoPEER, fluorescent prime editing and enrichment reporter, to rank the efficiency of pegRNAs and prime editor variants.
I. F. Schene +15 more
doaj +2 more sources
Evaluating CRISPR-based prime editing for cancer modeling and CFTR repair in organoids [PDF]
Life Science Alliance, 2021 Here, the authors evaluate prime editing in human adult stem cell–derived organoids. TP53 mutations are modeled in hepatocyte and colon organoids and the clinical application of prime editing is evaluated by repairing mutations in CFTR without genome ...
Maarten H Geurts +10 more
doaj +2 more sources
Prime Editing for Human Gene Therapy: Where Are We Now?
Cells, 2023 Gene therapy holds tremendous potential in the treatment of inherited diseases. Unlike traditional medicines, which only treat the symptoms, gene therapy has the potential to cure the disease by addressing the root of the problem: genetic mutations.
Kelly Godbout, Jacques P. Tremblay
doaj +2 more sources
Prime Editing: Mechanistic Insights and DNA Repair Modulation
CellsPrime editing is a genome editing technique that allows precise modifications of cellular DNA without relying on donor DNA templates. Recently, several different prime editor proteins have been published in the literature, relying on single- or double ...
Astrid Mentani +2 more
doaj +2 more sources
Reverting TP53 Mutation in Breast Cancer Cells: Prime Editing Workflow and Technical Considerations
Cells, 2022 Breast cancer is the leading cause of cancer-related deaths in women. The aggressive breast cancer subtype is commonly linked to the genetic alterations in the TP53 tumor suppressor gene, predominantly the missense mutations.
Asmaa Y. Abuhamad +8 more
doaj +2 more sources
Enhancement of prime editing by recruiting engineered or evolved components and implementing novel strategies [PDF]
Biochemistry and Biophysics ReportsPrime editing has recently gained attention for its promising potential in treating genetic disorders caused by different types of mutations. This method, based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), has led to the ...
Mobina Arabi +6 more
doaj +2 more sources