Results 1 to 10 of about 22,577 (244)
CRISPR-Cas systems as precision antimicrobials: Reversing the tide of antimicrobial resistance [PDF]
Virus ResearchAntimicrobial resistance (AMR) has escalated into a global health crisis, with resistant pathogens causing over 1.2 million direct deaths annually and threatening to render modern medicine unsustainable.
Mahsa Khosrojerdi +4 more
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Inducible CRISPR/Cas systems in precision oncology: Current applications and future perspectives [PDF]
Clinical and Translational MedicineBackground Inducible CRISPR/Cas systems enable spatiotemporal control of genome editing in response to chemical, optical, biological, or physical stimuli.
Ziliang Ding +3 more
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How to Find the Right RNA-Sensing CRISPR-Cas System for an In Vitro Application
Biosensors, 2022 CRISPR-Cas systems have a great and still largely untapped potential for in vitro applications, in particular, for RNA biosensing. However, there is currently no systematic guide on selecting the most appropriate RNA-targeting CRISPR-Cas system for a ...
Escarlet Díaz-Galicia +2 more
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The engineered single guide RNA structure as a biomarker for gene-editing reagent exposure
Scientific Reports, 2023 CRISPR arrays and CRISPR-associated (Cas) proteins comprise a prevalent adaptive immune system in bacteria and archaea. These systems defend against exogenous parasitic mobile genetic elements.
Emmarie C. Ryan +2 more
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Carrier strategies boost the application of CRISPR/Cas system in gene therapy
Exploration, 2022 Emerging clustered regularly interspaced short palindromic repeat/associated protein (CRISPR/Cas) genome editing technology shows great potential in gene therapy.
Zunkai Xu +10 more
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Guide RNAs: A Glimpse at the Sequences that Drive CRISPR–Cas Systems [PDF]
Cold Spring Harbor Protocols, 2016 CRISPR–Cas systems provide adaptive immunity in bacteria and archaea. Although there are two main classes of CRISPR–Cas systems defined by gene content, interfering RNA biogenesis, and effector proteins, Type II systems have recently been exploited on a broad scale to develop next-generation genetic engineering and genome-editing tools.
Alexandra E, Briner, Rodolphe, Barrangou
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CRISPR based therapeutics: a new paradigm in cancer precision medicine
Molecular Cancer, 2022 Background Clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein (Cas) systems are the latest addition to the plethora of gene-editing tools.
Sumit Das +3 more
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Frontiers in Microbiology, 2021 The type I clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system is one of five adaptive immune systems and exists widely in bacteria and archaea. In this study, we showed that Xanthomonas oryzae pv.
Qibing Liu +6 more
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RNA-guided editing of bacterial genomes using CRISPR-Cas systems [PDF]
Nature Biotechnology, 2013 Here we use the clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations in the genomes of Streptococcus pneumoniae and Escherichia coli. The approach relies on dual-RNA:Cas9-directed cleavage at the targeted genomic site to kill unmutated cells and ...
Jiang, Wenyan +4 more
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RNA-Guided Genome Editing in Plants Using a CRISPR–Cas System [PDF]
Molecular Plant, 2013 Precise and straightforward methods to edit the plant genome are much needed for functional genomics and crop improvement. Recently, RNA-guided genome editing using bacterial Type II cluster regularly interspaced short palindromic repeats (CRISPR)-associated nuclease (Cas) is emerging as an efficient tool for genome editing in microbial and animal ...
Xie, Kabin, Yang, Yinong
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