Expanding the Microbial Genomic Landscape and Biotechnological Applications of CRISPR-Cas Systems [PDF]
BiologyThe CRISPR-Cas systems, identified initially as adaptive immune mechanisms in bacteria and archaea against viral threats, have rapidly evolved into transformative tools in genetic engineering and biotechnology.
Swati Singh +5 more
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A leader-repeat hairpin blocks extraneous CRISPR RNA production in diverse CRISPR-Cas13 systems [PDF]
The EMBO JournalCRISPR RNAs (crRNAs) guide recognition and targeting of intracellular invaders as part of adaptive immunity by CRISPR-Cas systems. crRNAs are transcribed from CRISPR arrays of conserved repeats interlaced with invader-derived spacers.
Angela Migur +8 more
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A Rapid Hairy Root-Based Platform for CRISPR/Cas Optimization and Guide RNA Validation in Lettuce [PDF]
PlantsCultivated lettuce (Lactuca sativa L.) is a major leafy crop and an emerging model for functional genomics within the Asteraceae family, supported by high-quality reference genomes and efficient transformation systems.
Alberico Di Pinto +10 more
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How to Find the Right RNA-Sensing CRISPR-Cas System for an In Vitro Application
Biosensors, 2022 CRISPR-Cas systems have a great and still largely untapped potential for in vitro applications, in particular, for RNA biosensing. However, there is currently no systematic guide on selecting the most appropriate RNA-targeting CRISPR-Cas system for a ...
Escarlet Díaz-Galicia +2 more
doaj +1 more source
The engineered single guide RNA structure as a biomarker for gene-editing reagent exposure
Scientific Reports, 2023 CRISPR arrays and CRISPR-associated (Cas) proteins comprise a prevalent adaptive immune system in bacteria and archaea. These systems defend against exogenous parasitic mobile genetic elements.
Emmarie C. Ryan +2 more
doaj +1 more source
Carrier strategies boost the application of CRISPR/Cas system in gene therapy
Exploration, 2022 Emerging clustered regularly interspaced short palindromic repeat/associated protein (CRISPR/Cas) genome editing technology shows great potential in gene therapy.
Zunkai Xu +10 more
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Guide RNAs: A Glimpse at the Sequences that Drive CRISPR–Cas Systems [PDF]
Cold Spring Harbor Protocols, 2016 CRISPR–Cas systems provide adaptive immunity in bacteria and archaea. Although there are two main classes of CRISPR–Cas systems defined by gene content, interfering RNA biogenesis, and effector proteins, Type II systems have recently been exploited on a broad scale to develop next-generation genetic engineering and genome-editing tools.
Alexandra E, Briner, Rodolphe, Barrangou
openaire +2 more sources
CRISPR based therapeutics: a new paradigm in cancer precision medicine
Molecular Cancer, 2022 Background Clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein (Cas) systems are the latest addition to the plethora of gene-editing tools.
Sumit Das +3 more
doaj +1 more source
Frontiers in Microbiology, 2021 The type I clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system is one of five adaptive immune systems and exists widely in bacteria and archaea. In this study, we showed that Xanthomonas oryzae pv.
Qibing Liu +6 more
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RNA-guided editing of bacterial genomes using CRISPR-Cas systems [PDF]
Nature Biotechnology, 2013 Here we use the clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations in the genomes of Streptococcus pneumoniae and Escherichia coli. The approach relies on dual-RNA:Cas9-directed cleavage at the targeted genomic site to kill unmutated cells and ...
Jiang, Wenyan +4 more
openaire +4 more sources