How to Find the Right RNA-Sensing CRISPR-Cas System for an In Vitro Application
Biosensors, 2022 CRISPR-Cas systems have a great and still largely untapped potential for in vitro applications, in particular, for RNA biosensing. However, there is currently no systematic guide on selecting the most appropriate RNA-targeting CRISPR-Cas system for a ...
Escarlet Díaz-Galicia +2 more
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Expanding the Microbial Genomic Landscape and Biotechnological Applications of CRISPR-Cas Systems [PDF]
BiologyThe CRISPR-Cas systems, identified initially as adaptive immune mechanisms in bacteria and archaea against viral threats, have rapidly evolved into transformative tools in genetic engineering and biotechnology.
Swati Singh +5 more
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The engineered single guide RNA structure as a biomarker for gene-editing reagent exposure
Scientific Reports, 2023 CRISPR arrays and CRISPR-associated (Cas) proteins comprise a prevalent adaptive immune system in bacteria and archaea. These systems defend against exogenous parasitic mobile genetic elements.
Emmarie C. Ryan +2 more
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A leader-repeat hairpin blocks extraneous CRISPR RNA production in diverse CRISPR-Cas13 systems [PDF]
The EMBO JournalCRISPR RNAs (crRNAs) guide recognition and targeting of intracellular invaders as part of adaptive immunity by CRISPR-Cas systems. crRNAs are transcribed from CRISPR arrays of conserved repeats interlaced with invader-derived spacers.
Angela Migur +8 more
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Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems [PDF]
Biology Direct, 2011 Background The CRISPR-Cas adaptive immunity systems that are present in most Archaea and many Bacteria function by incorporating fragments of alien genomes into specific genomic loci, transcribing the inserts and using the transcripts as guide RNAs to ...
Wolf Yuri I +3 more
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Cytosolic CRISPR RNAs for efficient application of RNA-targeting CRISPR-Cas systems [PDF]
EMBO ReportsClustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) technologies have evolved rapidly over the past decade with the continuous discovery of new Cas systems.
Ezra C K Cheng, Joe K C Lam, S Chul Kwon
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Type I-E CRISPR-cas systems discriminate target from non-target DNA through base pairing-independent PAM recognition. [PDF]
PLoS Genetics, 2013 Discriminating self and non-self is a universal requirement of immune systems. Adaptive immune systems in prokaryotes are centered around repetitive loci called CRISPRs (clustered regularly interspaced short palindromic repeat), into which invader DNA ...
Edze R Westra +6 more
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Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration
Nature, 2019 Conventional CRISPR-Cas systems maintain genomic integrity by leveraging guide RNAs for the nuclease-dependent degradation of mobile genetic elements, including plasmids and viruses. Here we describe a notable inversion of this paradigm, in which bacterial Tn7-like transposons have co-opted nuclease-deficient CRISPR-Cas systems to catalyse RNA-guided ...
Sanne E, Klompe +3 more
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Unravelling the structural and mechanistic basis of CRISPR–Cas systems
Nature Reviews Microbiology, 2014 Bacteria and archaea have evolved sophisticated adaptive immune systems, known as CRISPR–Cas (clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins) systems, which target and inactivate invading viruses and plasmids ...
John Van Der Oost +2 more
exaly +3 more sources
GenesThe recent discovery of TIGR-Tas (Tandem Interspaced Guide RNA–Targeting Systems) marks a major advance in the field of genome editing, introducing a new class of compact, programmable DNA-targeting systems that function independently of traditional CRISPR-Cas pathways.
Douglas M. Ruden
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