Development and Application of CRISPR/Cas in Microbial Biotechnology
Frontiers in Bioengineering and Biotechnology, 2020 The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools with high efficiency, accuracy and flexibility, and has revolutionized traditional methods ...
Wentao Ding +3 more
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Target RNA-guided protease activity in type III-E CRISPR–Cas system
Nucleic Acids Research, 2022 Abstract The type III-E CRISPR–Cas systems are newly identified adaptive immune systems in prokaryotes that use a single Cas7–11 protein to specifically cleave target RNA. Cas7–11 could associate with Csx29, a putative caspase-like protein encoded by the gene frequently found in the type III-E loci, suggesting a functional linkage ...
Xiaoshen Wang +11 more
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Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System [PDF]
Cell, 2015 The microbial adaptive immune system CRISPR mediates defense against foreign genetic elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize multi-protein complexes, whereas class 2 effectors rely on single-component effector proteins such as the well-characterized Cas9.
Zetsche, Bernd +11 more
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Dynamic observations of CRISPR-Cas target recognition and cleavage heterogeneities
Nanophotonics, 2022 CRISPR-Cas systems (clustered regularly interspaced short palindromic repeats) have shown great potential as efficient gene editing tools in disease therapeutics.
Zhang Zhijia +12 more
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Structure of the Saccharolobus solfataricus type III-D CRISPR effector
Current Research in Structural Biology, 2023 CRISPR-Cas is a prokaryotic adaptive immune system, classified into six different types, each characterised by a signature protein. Type III systems, classified based on the presence of a Cas10 subunit, are rather diverse multi-subunit assemblies with a ...
Giuseppe Cannone +4 more
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Computational approaches for effective CRISPR guide RNA design and evaluation
Computational and Structural Biotechnology Journal, 2020 The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/ CRISPR-associated (Cas) system has emerged as the main technology for gene editing. Successful editing by CRISPR requires an appropriate Cas protein and guide RNA.
Guanqing Liu, Yong Zhang, Tao Zhang
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Endogenous CRISPR-Cas System-Based Genome Editing and Antimicrobials: Review and Prospects
Frontiers in Microbiology, 2019 CRISPR-Cas systems adapt “memories” via spacers from viruses and plasmids to develop adaptive immunity against mobile genetic elements. Mature CRISPR RNAs guide CRISPR-associated nucleases to site-specifically cleave target DNA or RNA, providing an ...
Yingjun Li, Nan Peng
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Type I-E CRISPR-cas systems discriminate target from non-target DNA through base pairing-independent PAM recognition. [PDF]
PLoS Genetics, 2013 Discriminating self and non-self is a universal requirement of immune systems. Adaptive immune systems in prokaryotes are centered around repetitive loci called CRISPRs (clustered regularly interspaced short palindromic repeat), into which invader DNA ...
Edze R Westra +6 more
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A Type IV-A CRISPR-Cas System in Pseudomonas aeruginosa Mediates RNA-Guided Plasmid Interference In Vivo [PDF]
The CRISPR Journal, 2019 Bacteria and archaea use CRISPR-Cas adaptive immune systems to destroy complementary nucleic acids using RNAs derived from CRISPR loci. Here, we provide the first functional evidence for type IV CRISPR-Cas, demonstrating that the system from Pseudomonas aeruginosa strain PA83 mediates RNA ...
Valerie M, Crowley +6 more
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CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks
eLife, 2020 Type V CRISPR-Cas interference proteins use a single RuvC active site to make RNA-guided breaks in double-stranded DNA substrates, an activity essential for both bacterial immunity and genome editing.
Joshua C Cofsky +5 more
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