Results 1 to 10 of about 64,056 (293)
Biomolecules, 2023 Programmable endonucleases, such as Cas (Clustered Regularly-Interspaced Short Repeats-associated proteins) and prokaryotic Argonaute (pAgo), depend on base pairing of the target DNA with the guide RNA or DNA to cleave DNA strands.
Xingchen Xiong +3 more
doaj +2 more sources
Fluorescent Analysis of KM and Vmax Values for Methyl-Dependent Restriction Endonucleases [PDF]
BiomoleculesMethyl-dependent restriction endonucleases are promising tools for analyzing eukaryotic DNA methylation patterns. However, quantitative assessment of their substrate specificity requires the determination of the kinetic parameters of enzymatic reactions.
Vladislava Martyshova, Sergey Sedykh
doaj +2 more sources
Continuous secretory production in E. coli enables scalable, high-titer manufacturing of active recombinant endonucleases [PDF]
Journal of Biological EngineeringRecombinant endonucleases are essential for biopharmaceutical manufacturing and molecular biology workflows, yet their intracellular expression in Escherichia coli often leads to host cell toxicity due to non-specific DNA and RNA degradation.
Sudarsana Reddy Lokireddy +6 more
doaj +2 more sources
A new Caenorhabditis elegans apurinic/apyrimidinic (AP) endonuclease engaged in rescue from replication stress-induced arrest [PDF]
Genetics and Molecular BiologyApurinic/apyrimidinic sites are one of the most frequent spontaneous lesions in DNA. Evolutionarily conserved AP endonucleases (ExoIII and EndoIV families) incise the DNA backbone 5′ to the AP site and the cleaved AP sites are subsequently repaired by ...
Seoyun Choi, Hoa Thi Pham, Byungchan Ahn
doaj +2 more sources
To cleave or not and how? The DNA exonucleases and endonucleases in immunity
Genes and DiseasesDNA exonucleases and endonucleases are key executors of the genome during many physiological processes. They generate double-stranded DNA by cleaving damaged endogenous or exogenous DNA, triggering the activation of the innate immune pathways such as ...
Changming An, Teng Ma, Jinghong Wu
exaly +3 more sources
A new view of RNA: the 1989 discovery by Sidney Altman and Thomas Cech [PDF]
The Ukrainian Biochemical Journal, 2020 The 1989 Nobel Prize Laureates in Chemistry Sidney Altman and Thomas Robert Cech made one of the most important discoveries in molecular genetics. Independently of each other, they demonstrated new experimental evidence that RNA molecules can not only ...
M. V. Grigorieva +2 more
doaj +1 more source
Biomolecules, 2021 The prokaryotic Argonaute proteins (pAgos) have been reported to cleave or interfere with DNA targets in a guide-dependent or independent manner. It is often difficult to characterize pAgos in vivo due to the extreme environments favored by their hosts ...
Jiani Xing +7 more
doaj +1 more source
Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia [PDF]
Ratarstvo i Povrtarstvo, 2017 During 2008-2010 the infected Brassica napus plant material was collected from nine localities: Karavukovo, Crvenka, Prigrevica, Subotica, Rimski Šančevi, Srbobran, Beška, Banatsko Karađorđevo, and Srpski Miletić.
Mitrović Petar +3 more
doaj +1 more source
Biochemistry (Moscow), 2009 Nicking endonucleases are a new type of enzymes. Like restriction endonucleases, they recognize short specific DNA sequence and cleave DNA at a fixed position relatively to the recognition sequence. However, unlike restriction endonucleases, nicking endonucleases cleave only one predetermined DNA strand.
Zheleznaya, L. A. +5 more
openaire +2 more sources
Recent insights into the structure and function of coronavirus ribonucleases
FEBS Open Bio, 2022 Coronaviruses use approximately two‐thirds of their 30‐kb genomes to encode nonstructural proteins (nsps) with diverse functions that assist in viral replication and transcription, and evasion of the host immune response.
Meredith N. Frazier +4 more
doaj +1 more source