Results 1 to 10 of about 60,825 (304)
Biomolecules, 2023 Programmable endonucleases, such as Cas (Clustered Regularly-Interspaced Short Repeats-associated proteins) and prokaryotic Argonaute (pAgo), depend on base pairing of the target DNA with the guide RNA or DNA to cleave DNA strands.
Xingchen Xiong +3 more
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BMC Microbiology, 2018 Background The functioning of DNA repair systems is based on correct interactions between proteins involved in DNA repair. Very Short Patch (VSP) repair is a DNA repair system that corrects mismatches resulting from the deamination of 5-methylcytosine ...
Monika Adamczyk-Popławska +2 more
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Continuous secretory production in E. coli enables scalable, high-titer manufacturing of active recombinant endonucleases [PDF]
Journal of Biological EngineeringRecombinant endonucleases are essential for biopharmaceutical manufacturing and molecular biology workflows, yet their intracellular expression in Escherichia coli often leads to host cell toxicity due to non-specific DNA and RNA degradation.
Sudarsana Reddy Lokireddy +6 more
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A new Caenorhabditis elegans apurinic/apyrimidinic (AP) endonuclease engaged in rescue from replication stress-induced arrest [PDF]
Genetics and Molecular BiologyApurinic/apyrimidinic sites are one of the most frequent spontaneous lesions in DNA. Evolutionarily conserved AP endonucleases (ExoIII and EndoIV families) incise the DNA backbone 5′ to the AP site and the cleaved AP sites are subsequently repaired by ...
Seoyun Choi, Hoa Thi Pham, Byungchan Ahn
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Fitness effects of CRISPR endonucleases in Drosophila melanogaster populations
eLife, 2022 Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 provides a highly efficient and flexible genome editing technology with numerous potential applications ranging from gene therapy to population control.
Anna M Langmüller +10 more
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A new view of RNA: the 1989 discovery by Sidney Altman and Thomas Cech [PDF]
The Ukrainian Biochemical Journal, 2020 The 1989 Nobel Prize Laureates in Chemistry Sidney Altman and Thomas Robert Cech made one of the most important discoveries in molecular genetics. Independently of each other, they demonstrated new experimental evidence that RNA molecules can not only ...
M. V. Grigorieva +2 more
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Small molecule inhibitors of RNA guided endonucleases [PDF]
, 2021 The present invention relates to the use of compounds of formula (I) as inhibitors of RNA guided endonucleases, e.g., Cas9 and Cpfl, and methods for the inhibition of the function of such RNA guided endonucleases, including their use in the treatment and
Liu, Bin +3 more
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Biomolecules, 2021 The prokaryotic Argonaute proteins (pAgos) have been reported to cleave or interfere with DNA targets in a guide-dependent or independent manner. It is often difficult to characterize pAgos in vivo due to the extreme environments favored by their hosts ...
Jiani Xing +7 more
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Molecular characterization and identification of fungi causing stern canker of oilseed rape in Serbia [PDF]
Ratarstvo i Povrtarstvo, 2017 During 2008-2010 the infected Brassica napus plant material was collected from nine localities: Karavukovo, Crvenka, Prigrevica, Subotica, Rimski Šančevi, Srbobran, Beška, Banatsko Karađorđevo, and Srpski Miletić.
Mitrović Petar +3 more
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Biochemistry (Moscow), 2009 Nicking endonucleases are a new type of enzymes. Like restriction endonucleases, they recognize short specific DNA sequence and cleave DNA at a fixed position relatively to the recognition sequence. However, unlike restriction endonucleases, nicking endonucleases cleave only one predetermined DNA strand.
Zheleznaya, L. A. +5 more
openaire +2 more sources