Results 11 to 20 of about 60,825 (304)

Recent insights into the structure and function of coronavirus ribonucleases

FEBS Open Bio, 2022
Coronaviruses use approximately two‐thirds of their 30‐kb genomes to encode nonstructural proteins (nsps) with diverse functions that assist in viral replication and transcription, and evasion of the host immune response.
Meredith N. Frazier, Amanda A. Riccio, Isha M. Wilson, William C. Copeland, Robin E. Stanley   +4 more
doaj   +1 more source

Rolling Circle Enhanced Detection of Specific Restriction Endonuclease Activities in Crude Cell Extracts

Sensors, 2022
Restriction endonucleases are expressed in all bacteria investigated so far and play an essential role for the bacterial defense against viral infections.
Kamilla Vandsø Petersen, Cinzia Tesauro, Marianne Smedegaard Hede, Camilla Pages, Lærke Bay Marcussen, Josephine Geertsen Keller, Magnus Bugge, Kasper Holm, Lotte Bjergbæk, Magnus Stougaard, Christian Wejse, Birgitta R. Knudsen   +11 more
doaj   +1 more source

Isolation, cloning and molecular characterization of genes encoding single-strand specific endonucleases from Parsley (Petroselinum crispum L.) [PDF]

مجله بیوتکنولوژی کشاورزی, 2015
Single-strand specific (sss) nucleases (S1/P1 family) are widely used in molecular genetics studies. The most widely used sss-nucleases are those plant endonucleases with the ability of specific cleavage of mismatch loops in heteroduplex DNA molecules ...
Mahdiyeh Mirzaei, Jafar Zol Ala, Anahit Shalouzad   +2 more
doaj   +1 more source

Crystal structures of Lymphocytic choriomeningitis virus endonuclease domain complexed with diketo-acid ligands

IUCrJ, 2018
The Arenaviridae family, together with the Bunyaviridae and Orthomyxoviridae families, is one of the three negative-stranded RNA viral families that encode an endonuclease in their genome.
Magali Saez-Ayala, Elsie Laban Yekwa, Mauro Carcelli, Bruno Canard, Karine Alvarez, François Ferron   +5 more
doaj   +1 more source

Endonuclease activity in lipocalins [PDF]

Biochemical Journal, 2000
Several lipocalins contain conserved amino acid sequences similar to the phosphodiester bond cleavage domain of sugar non-specific magnesium-dependent nucleases of the Serratia marcescens type. His-89 and Glu-127 of the S. marcescens endonuclease are believed to have a role in the active catalytic site by the attack of a water molecule at the ...
T N, Yusifov, A R, Abduragimov, O K, Gasymov, B J, Glasgow   +3 more
openaire   +2 more sources

Variabilidade genética na região its do rDNA de isolados de trichoderma spp. (Biocontrolador) e Fusarium oxysporum f. sp. Chrysanthemi Genetic variability in rDNA ITS region of Trichoderma spp. (biocontrole agent) and Fusarium oxysporum f. sp. chrysanthemi isolates

Ciência e Agrotecnologia, 2010
A análise de características morfológicas e culturais podem não ser suficientes para uma caracterização precisa das espécies de Trichoderma e Fusarium.
Josiane Pacheco Menezes, Manoeli Lupatini, Zaida Inês Antoniolli, Elena Blume, Emanuele Junges, Clarice G. Manzoni   +5 more
doaj   +1 more source

Historical Aspects of Restriction Endonucleases as Intelligent Scissors for Genetic Engineering

Fermentation, 2023
Restriction endonucleases are a component of restriction–modification systems, where the main biological function is to protect bacterial cells from incoming foreign DNA molecules.
Irina V. Alekseeva, Nikita A. Kuznetsov
doaj   +1 more source

Characterization of immobilized biomass by amplified rDNA restriction analysis (ARDRA) in an anaerobic sequencing-batch biofilm reactor (ASBBR) for the treatment of industrial wastewater

Brazilian Archives of Biology and Technology, 2012
The performance of an anaerobic sequencing-batch biofilm reactor (ASBBR- laboratory scale- 14L )containing biomass immobilized on coal was evaluated for the removal of elevated concentrations of sulfate (between 200 and 3,000 mg SO4-2·L-1) from ...
Arnaldo Sarti, Eloisa Pozzi, Marcelo Zaiat   +2 more
doaj   +1 more source

Pre-Ribosomal RNA Processing in Human Cells: From Mechanisms to Congenital Diseases

Biomolecules, 2018
Ribosomal RNAs, the most abundant cellular RNA species, have evolved as the structural scaffold and the catalytic center of protein synthesis in every living organism.
Maxime Aubert, Marie-Françoise O’Donohue, Simon Lebaron, Pierre-Emmanuel Gleizes   +3 more
doaj   +1 more source

Divalent metal ion differentially regulates the sequential nicking reactions of the GIY-YIG homing endonuclease I-BmoI. [PDF]

PLoS ONE, 2011
Homing endonucleases are site-specific DNA endonucleases that function as mobile genetic elements by introducing double-strand breaks or nicks at defined locations.
Benjamin P Kleinstiver, Wesley Bérubé-Janzen, Andrew D Fernandes, David R Edgell   +3 more
doaj   +1 more source