Exonucleases: Degrading DNA to Deal with Genome Damage, Cell Death, Inflammation and Cancer [PDF]
Cells, 2022 Although DNA degradation might seem an unwanted event, it is essential in many cellular processes that are key to maintaining genomic stability and cell and organism homeostasis.
Joan Manils +2 more
doaj +2 more sources
Selective Inhibition of Proofreading Exonucleases: The Central Role in Obesity-Associated Carcinogenesis [PDF]
Current Issues in Molecular BiologyObesity-associated carcinogenesis offers a model to explore the transition from metabolic dysregulation to genomic instability and carcinogenesis.
John J. Byrnes
doaj +2 more sources
Viruses, 2023 Nucleases are ubiquitous hydrolytic enzymes that cleave phosphodiester bond of DNA (DNases), RNA (RNases), or protein-RNA/DNA (phosphodiesterases), within the strand (endonucleases) or from the end (exonucleases) [...]
Zhengqiang Wang, Robert J. Geraghty
doaj +2 more sources
To cleave or not and how? The DNA exonucleases and endonucleases in immunity
Genes and DiseasesDNA exonucleases and endonucleases are key executors of the genome during many physiological processes. They generate double-stranded DNA by cleaving damaged endogenous or exogenous DNA, triggering the activation of the innate immune pathways such as ...
Changming An, Teng Ma, Jinghong Wu
exaly +3 more sources
Adapting prime editing with split prime editors in Escherichia coli and its application to Staphylococcus aureus genome editing [PDF]
Applied Microbiology and BiotechnologyPrime editing is a precise and rapid genome-editing technique that modifies short DNA sequences using tailored guide RNAs. To implement this technique in bacteria, we used Prime Editor 2 (PE2) with the DeepPrime gRNA design tool and assessed its gene ...
Seong Hyeok Ma +7 more
doaj +2 more sources
5PSeq Explorer: interactive analysis of co-translational mRNA decay and ribosome dynamics [PDF]
RNA BiologyCo-translational mRNA decay occurs when 5’to3’ exonucleases follow the last translating ribosome, generating in vivo ribosome protected fragments. Degradome sequencing (5PSeq)therefore offers unique insights into ribosome dynamics. Despite its potential,
Irene Stevens, Vicent Pelechano
doaj +2 more sources
Mechanistic insights into single-stranded DNA degradation by lysosomal exonucleases PLD3 and PLD4 from structural snapshots [PDF]
Nature CommunicationsLysosomal exonuclease phospholipase D (PLD) family PLD3 and PLD4 degrade single-stranded RNA or DNA and regulate TLR7 or TLR9 responses. Polymorphisms of these enzymes are associated with human diseases: PLD4 is associated with inflammatory diseases, and
Yoshinori Hirano +5 more
doaj +2 more sources
Dimerization-dependent gel-like condensation with dsDNA underpins the activation of human cGAS [PDF]
Cell ReportsSummary: Cyclic G/AMP (cGAMP) synthase (cGAS) initiates inflammatory responses against pathogenic double-stranded (ds)DNA. Although it is well established that cGAS forms phase-separated condensates with dsDNA, its function remains poorly defined.
Jacob Lueck +7 more
doaj +2 more sources
Viruses, 2022 In this review, we explore recombination in two very different virus families that have become major threats to human health. The Herpesviridae are a large family of pathogenic double-stranded DNA viruses involved in a range of diseases affecting both ...
Lee R. Wright +2 more
doaj +1 more source
Inhibition of Arenaviridae nucleoprotein exonuclease by bisphosphonate
IUCrJ, 2022 Arenaviruses are emerging enveloped negative-sense RNA viruses that cause neurological and hemorrhagic diseases in humans. Currently, no FDA-approved vaccine or therapeutic agent is available except for ribavirin, which must be administered early during ...
Thi Hong Van Nguyen +5 more
doaj +1 more source