Results 1 to 10 of about 2,121 (83)

Exodeoxyribonuclease from rat brain specific for single‐stranded DNA [PDF]

European Journal of Biochemistry, 1983
An exodeoxynuclease with single‐stranded DNA specificity has been isolated from rat brain and purified to electrophoretic homogeneity. Approximately 1100‐fold purification with a yield of 16% has been achieved by chromatography on DNA‐agarose, hydroxyapatite and Sephadex G‐200. The enzyme has a pH optimum at 8.4, requires Mg2+ or Mn2+ as a cofactor and
V A, Ivanov, A I, Gaziev, T M, Tretyak
openaire   +2 more sources

Purification and characterization of a novel 5′ exodeoxyribonuclease from the yeast Saccharomyces cerevisiae [PDF]

European Journal of Biochemistry, 1991
We have isolated from yeast cells an exonuclease which preferentially attacks double‐stranded DNA from the 5′ ends producing 5′‐mononucleotides as reaction products. A second typical product is a full‐length single‐stranded DNA complement, suggesting that the enzyme hydrolyzes one DNA strand in a processive manner before it associates with another DNA ...
M, Dölberg, C P, Baur, R, Knippers
openaire   +2 more sources

On the fidelity of DNA replication. Lack of exodeoxyribonuclease activity and error-correcting function in avian myeloblastosis virus DNA polymerase.

Journal of Biological Chemistry, 1976
Homogeneous DNA polymerase ("reverse transcriptase") from avian myeoblastosis virus was assayed for exodeoxyribonuclease activity. The substrates were defined template-initiator complexes in which different radioactive nucleotides were present at the 3'-OH termini of the initiator.
N, Battula, L A, Loeb
openaire   +2 more sources

Purification, Crystallization and Preliminary X-Ray Diffraction Analysis of Exodeoxyribonuclease III from Crenarchaeon <i>Sulfolobus tokodaii</i> Strain 7

Crystal Structure Theory and Applications, 2013
Exodeoxyribonuclease III (EXOIII) acts as a 3’→5’ exonuclease and is homologous to purinic/apyrimidinic (AP) endonuclease (APE), which plays an important role in the base excision repair pathway. To structurally investigate the reaction and substrate recognition mechanisms of EXOIII, a crystallographic study of EXOIII from Sulfolobus tokodaii strain 7 ...
Shuichi Miyamoto, Chieko Naoe, Masaru Tsunoda, Kazuo T. Nakamura   +3 more
openaire   +2 more sources

Human APE2 and TREX2 Repair 3'-DNA-Peptide Cross-links Derived from Abasic Sites. [PDF]

Chem Res Toxicol
Wei X, Cepeda J, Chung JM, Lee SE, Yang K.   +4 more
europepmc   +1 more source

Highly Sensitive Fluorescent Detection of HPV-16 DNA Using Tungsten Disulfide Nanosheets and Exonuclease III-Assisted Signal Amplification. [PDF]

Biosensors (Basel)
Wu M, Lin G, Dong J, Zeng A, Hong H, Chen Z, Hong C.   +6 more
europepmc   +1 more source

Exploring targeted therapy in retinal vasculopathy with cerebral leukoencephalopathy: a case report and review of literature. [PDF]

Front Immunol
Tato-Moreno P, Lavilla Olleros C, Balastegui Martín H, Barrientos Guerrero M, Mensa-Vilaro A, Durán-García ME, Sánchez-Mateos P, García-Martínez E.   +7 more
europepmc   +1 more source

First case of <i>TREX1</i> mutation-driven retinal vasculopathy with cerebral leukoencephalopathy and systemic manifestations coexisting with lupus nephritis: a case report and mechanistic discussion. [PDF]

Front Immunol
Hao W, Zhai S, Zhu Q, Zhou X, Shen T, He W, Sun Y, Yang W, Yang Y.   +8 more
europepmc   +1 more source

Structure of an initiation complex of mycobacterial helicase-nuclease AdnAB at a blunt double-strand break reveals local melting that engages the 3' tracking strand at the ratchet pawl of the helicase motor. [PDF]

Nucleic Acids Res
Warren GM, Jason de la Cruz M, Goldgur Y, Shuman S.   +3 more
europepmc   +1 more source

Structure of human phospholipase D3, a single-strand exonuclease associated with Alzheimer's disease. [PDF]

FEBS J
Bijelic A, Macheroux P.
europepmc   +1 more source