Results 31 to 40 of about 18,119 (268)
PLoS Biology, 2014 DNA repair mechanisms are essential for preservation of genome integrity. However, it is not clear how DNA are selected and processed at broken ends by exonucleases during repair pathways.
Yu-Yuan Hsiao +4 more
doaj +1 more source
Translesion synthesis in mammalian cells [PDF]
, 2006 DNA damage blocks the progression of the replication fork. In order to circumvent the damaged bases, cells employ specialized low stringency DNA polymerases, which are able to carry out translesion synthesis (TLS) past different types of damage. The five
Alan R. Lehmann +35 more
core +2 more sources
LTR-retrotransposons from Bdelloid rotifers capture additional ORFs shared between highly diverse retroelement types [PDF]
, 2017 © The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Viruses 9 (2017): 78, doi:10.3390/v9040078.Rotifers of the class Bdelloidea, microscopic freshwater ...
Arkhipova, Irina R. +2 more
core +1 more source
PLoS Biology, 2017 The degradation of small RNAs in plants and animals is associated with small RNA 3' truncation and 3' uridylation and thus relies on exonucleases and nucleotidyl transferases.
Yu Yu +12 more
doaj +1 more source
Bio-Protocol, 2018 This protocol is designed to obtain base-resolution information on the level of 5-hydroxymethylcytosine (5hmC) in CpGs without the need for bisulfite modification.
Aurelien Serandour +2 more
doaj +1 more source
One-pot making of sequence-restricted DNA dumbbells
Heliyon, 2023 A method to build sequence-restricted DNA dumbbells was developed. 5′-exonuclease converts end sequences of DNA targets into sticky ends. Self-looping oligonucleotides with complementary 3′-overhangs are ligated to form dumbbells by DNA polymerase and ...
Ya-Hui Chang +2 more
doaj +1 more source
TREX1 is expressed by microglia in normal human brain and increases in regions affected by ischemia [PDF]
, 2018 BACKGROUND: Mutations in the three-prime repair exonuclease 1 (TREX1) gene have been associated with neurological diseases, including Retinal Vasculopathy with Cerebral Leukoencephalopathy (RVCL).
Atkinson, John P +6 more
core +3 more sources
Cassette hybridization for vector assembly application in antibody chain shuffling
BioTechniques, 2018 Gene assembly methods are an integral part of molecular cloning experiments. The majority of existing vector assembly methods stipulate a need for exonucleases, endonucleases and/or the use of single-stranded DNA as starting materials.
Jing Yi Lai +3 more
doaj +1 more source
Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases [PDF]
, 2013 Lambda Red recombineering is a powerful technique for making targeted genetic changes in bacteria. However, many applications are limited by the frequency of recombination.
Church, George McDonald +4 more
core +1 more source
Cell Reports, 2017 Non-coding RNA biogenesis in higher eukaryotes has not been fully characterized. Here, we studied the Drosophila melanogaster Rexo5 (CG8368) protein, a metazoan-specific member of the DEDDh 3′-5′ single-stranded RNA exonucleases, by genetic, biochemical,
Stefanie Gerstberger +8 more
doaj +1 more source