Results 51 to 60 of about 12,706 (198)

circZNF148 Drives Glucose Metabolism Reprogramming to Enhance Metastasis and Immune Evasion via HK1 Stabilization in Triple‐Negative Breast Cancer

Advanced Science, EarlyView.
CircZNF148 stabilizes HK1 through deubiquitinase recruitment, thereby enhancing glycolysis and lactate production. Elevated lactate promotes PD‑L1 lactylation and membrane accumulation while suppressing CD8+ T‐cell cytotoxicity, collectively facilitating immune evasion and malignant progression in TNBC.
Yuhan Jin, Wenjing Zhao, Lei Wang, Jianing Wang, Keying Zhao, Dan Luo, Yifei Wang, Yuxia Yang, Jingze Yang, Yaming Li, Tong Chen, Dianwen Han, Zekun Wang, Xiaoyan Li, Bing Chen, Lijuan Wang, Yiran Liang, Qifeng Yang   +17 more
wiley   +1 more source

Rapid Exonuclease‐Based De Novo Sequencing Strategy for Long Oligonucleotides via Liquid‐Phase Ion Trap Coupled With Mass Spectrometry

Advanced Science, EarlyView.
A liquid‐phase ion trap (LPIT) coupled with ESI‐MS enables rapid, high‐fidelity de novo sequencing of long oligonucleotides in ∼30 min. By applying an electric field to dissociate metal adducts in solution, LPIT delivers clean spectra, superior mass accuracy, and full sequence coverage, surpassing MALDI‐TOF and LC‐MS.
Song Lu, Jie Hong, Xinyan Fu, Di Wang, Yunhua Gao, Wei Xu   +5 more
wiley   +1 more source

Profiling of the Predicted Circular RNAs in Ductal In Situ and Invasive Breast Cancer: A Pilot Study

International Journal of Genomics, 2016
The recent advantage obtained by next generation sequencing allows a depth investigation of a new “old” kind of noncoding transcript, the circular RNAs. Circular RNAs are nontranslated RNAs, typically nonpolyadenylated, with a resistance to exonucleases ...
Marco Galasso, Giorgio Costantino, Lorenzo Pasquali, Linda Minotti, Federica Baldassari, Fabio Corrà, Chiara Agnoletto, Stefano Volinia   +7 more
doaj   +1 more source

Half a century after their discovery: Structural insights into exonuclease and annealase proteins catalyzing recombineering

Engineering Microbiology
Recombineering is an essential tool for molecular biologists, allowing for the facile and efficient manipulation of bacterial genomes directly in cells without the need for costly and laborious in vitro manipulations involving restriction enzymes.
Lucy J. Fitschen, Timothy P. Newing, Nikolas P. Johnston, Charles E. Bell, Gökhan Tolun   +4 more
doaj   +1 more source

Restoration of m6A‐Modified circPSMB1 Suppresses Chordoma Progression and Enables Local Nanotherapeutic Intervention

Advanced Science, EarlyView.
An m6A‐modified circRNA, circPSMB1, is identified as a tumor suppressor in chordoma. METTL3‐dependent m6A promotes circPSMB1 interaction with HNRNPA2B1, limiting c‐MYC expression and malignant progression. An injectable HMnO2‐based nanohydrogel enables local circPSMB1 delivery and tumor microenvironment‐responsive therapy, offering a promising strategy
Yingchuang Tang, Liang Qiu, Xingbang Ruan, Yihan Shi, Ye Wang, Hanwen Li, Kai Zhang, Kangwu Chen   +7 more
wiley   +1 more source

A signal transmission strategy driven by gap-regulated exonuclease hydrolysis for hierarchical molecular networks

Communications Biology
Exonucleases serve as efficient tools for signal processing and play an important role in biochemical reactions. Here, we identify the mechanism of cooperative exonuclease hydrolysis, offering a method to regulate the cooperative hydrolysis driven by ...
Xin Liu, Xun Zhang, Shuang Cui, Shujuan Xu, Rongming Liu, Bin Wang, Xiaopeng Wei, Qiang Zhang   +7 more
doaj   +1 more source

Quinolones: a class of antimicrobial agents

Veterinární Medicína, 2001
The fluoroquinolones are a series of synthetic antibacterial agents that are used in the treatment of a variety of bacterial infections. These agents inhibit the DNA gyrase, abolishing its activity by interfering with the DNA-rejoining reaction.
G. Sárközy
doaj   +1 more source

Identification of PARN nuclease activity inhibitors by computational-based docking and high-throughput screening

Scientific Reports, 2023
Poly(A)-specific ribonuclease (PARN) is a 3′-exoribonuclease that removes poly(A) tails from the 3′ end of RNAs. PARN is known to deadenylate some ncRNAs, including hTR, Y RNAs, and some miRNAs and thereby enhance their stability by limiting the access ...
Thao Ngoc Huynh, Siddharth Shukla, Philip Reigan, Roy Parker   +3 more
doaj   +1 more source

Distinct Cleavage Trajectories Mediated by DNA and RNA Substrate in Argonaute From Methanocaldococcus Fervens

Advanced Science, EarlyView.
This study characterizes MfAgo, an enzyme exhibiting highly efficient DNA‐guided DNA and RNA cleavage capability. Precise RNA cleavage strictly requires dimerization to stabilize the PIWI‐loop region, while its robust DNA cleavage functions in a dimerization‐independent manner.
Fuyong Huang, Taiyu Chen, Meng Fang, Qinmiao Duanmu, Kangle Mu, Yuqi Bi, Siwei Xu, Xin Tao, Jiening Wang, Shan Wu   +9 more
wiley   +1 more source

RNA Degradation in Staphylococcus aureus: Diversity of Ribonucleases and Their Impact

International Journal of Genomics, 2015
The regulation of RNA decay is now widely recognized as having a central role in bacterial adaption to environmental stress. Here we present an overview on the diversity of ribonucleases (RNases) and their impact at the posttranscriptional level in the ...
Rémy A. Bonnin, Philippe Bouloc
doaj   +1 more source