Results 61 to 70 of about 12,706 (198)

In Situ, Real-Time Monitoring of the 3′ to 5′ Exonucleases Secreted by Living Cells

, 2016
Enzymes containing 3′-5′ exonuclease activities play vital roles in maintaining genome stability. Though a wide variety of methods have been developed for detection of these enzymes, few of them can be directly applied for in situ and real-time ...
Xiaocui Zhu (1898533), Chen Zhang (66790), Xianjin Xiao (1898530), Xin Su (338394), Meiping Zhao (1461175)   +4 more
core   +1 more source

Structure and inhibition of the human SNM1a and SNM1b/Apollo DNA repair exonucleases [PDF]

, 2015
The human SNM1A and SNM1B enzymes are 5'-3' exonucleases implicated in DNA interstrand crosslink (ICL) repair. Cells deficient in SNM1A or SNM1B show increased sensitivity to clinically important crosslinking drugs such as mitomycin C (MMC).
Sook Yee Lee, Lee, Sook Yee
core   +1 more source

Degradation of mRNAs in budding yeast by novel decay pathways involving the REX exonucleases [PDF]

, 2015
We searched through gene expression databases for long 3’ UTR mRNAs that are targeted for degradation by the cytoplasmic mRNA degradation pathway, nonsense-mediated mRNA decay (NMD) (Sayani et al., 2008).
Hodko, Domagoj
core  

DATEL: A Scarless and Sequence-Independent DNA Assembly Method Using Thermostable Exonucleases and Ligase

, 2016
DNA assembly is a pivotal technique in synthetic biology. Here, we report a scarless and sequence-independent DNA assembly method using thermal exonucleases (Taq and Pfu DNA polymerases) and Taq DNA ligase (DATEL).
Guocheng Du (390526), Peng Jin (216301), Wenwen Ding (2811643), Zhen Kang (335450), Jian Chen (15340)   +4 more
core   +1 more source

In Situ, Real-Time Monitoring of the 3 ' to 5 ' Exonucleases Secreted by Living Cells

, 2012
Enzymes containing 3'-5' exonuclease activities play vital roles in maintaining genome stability. Though a wide variety of methods have been developed for detection of these enzymes, few of them can be directly applied for in situ and real-time
Xiao, Xianjin, Zhang, Chen, Zhao, Meiping, Su, Xin, Zhu, Xiaocui   +4 more
core   +1 more source

Recent Advances in Virus–Host Interactions, Antiviral Bioactive Compounds, and Breeding for Disease Resistance of Porcine Epidemic Diarrhea Virus

Animal Research and One Health, EarlyView.
Basic research on the PEDV infection cycle and virus–host interactions advances the development of anti‐PEDV drugs and disease‐resistant breeding and helps strengthen disease prevention and control while reducing economic losses in the swine industry.
Heyong Wu, Aosi Qi, Shuyu Zhong, Haozhen Du, Shimeng Guo, Hongsheng Ouyang, Hongming Yuan, Daxin Pang, Zicong Xie   +8 more
wiley   +1 more source

Purification of Advanced Therapeutics by Flow‐Through Pseudo‐Affinity Chromatography: Bi‐Specific mABs, Fc Fusion Proteins, and Adeno‐Associated Viruses

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Modern biopharmaceutical manufacturing requires purification platforms capable of processing structurally and functionally diverse products while addressing the challenge of removing persistent and high‐risk host cell proteins (HCPs).
Wenning Chu, Eduardo Barbieri, Arianna Minzoni, Shriarjun Shastry, Jacob P. LeBarre, Qingbo Shu, Taufika Williams, Xuanwen Li, Divya Chandra, Xuankuo Xu, Jerome Bill, David Roush, Joseph Taris, Patrick Gilbert, Andre Kiesewetter, Oliver Rammo, Michael Schulte, Christopher Major, Trina Mouchahoir, Steven Cramer, Cristiana Boi, Ruben G. Carbonell, Abraham Lenhoff, Stefano Menegatti   +23 more
wiley   +1 more source

Correction of DNA polymerase mistakes and 3'→5'-exonucleases of the rat liver [PDF]

, 1985
Одним из уровней исправления ДНК-полимеразных ошибок является 3'→5'-экзонуклеазная «редакторская» активность, отсутствующая или неэффективная у ДНК-полимераз млекопитающих.
Тимченко, Н.А., Клейнер, Н.Е., Легина, О.К., Кравецкая, Т.П., Крутяков, В.М., Белякова, Н.В.   +5 more
core   +1 more source

Scalable Separation and Purification of M13‐Bacteriophage‐Derived Particles Containing Artificial Single‐Stranded DNA Produced by Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Single‐stranded DNA (ssDNA) solutions are promising innovations shaping the future of genetic research and medical therapies. In principle, biotechnological mass production of ssDNA can be achieved using Escherichia coli in a high‐cell density fed‐batch process by secreting phagemid particles derived from the filamentous M13 bacteriophages ...
Nathalie Hafner, Wiebke Winkler, Maximilian N. Honemann, Hendrik Dietz, Dirk Weuster‐Botz   +4 more
wiley   +1 more source

PCNA stimulates catalysis by structure-specific nucleases using two distinct mechanisms : substrate targeting and catalytic step [PDF]

, 2008
This work was supported by the Biotechnology and Biological Sciences Research Council [grants BBD0014391 and BBE0146741]The sliding clamp Proliferating Cell Nuclear Antigen (PCNA) functions as a recruiter and organizer of a wide variety of DNA modifying ...
Hutton, R D, White, Malcolm F, Roberts, J A, Penedo, Carlos   +3 more
core   +1 more source