Results 61 to 70 of about 18,119 (268)

Profiling of the Predicted Circular RNAs in Ductal In Situ and Invasive Breast Cancer: A Pilot Study

International Journal of Genomics, 2016
The recent advantage obtained by next generation sequencing allows a depth investigation of a new “old” kind of noncoding transcript, the circular RNAs. Circular RNAs are nontranslated RNAs, typically nonpolyadenylated, with a resistance to exonucleases ...
Marco Galasso, Giorgio Costantino, Lorenzo Pasquali, Linda Minotti, Federica Baldassari, Fabio Corrà, Chiara Agnoletto, Stefano Volinia   +7 more
doaj   +1 more source

Protecting the proteome: Eukaryotic cotranslational quality control pathways. [PDF]

, 2014
The correct decoding of messenger RNAs (mRNAs) into proteins is an essential cellular task. The translational process is monitored by several quality control (QC) mechanisms that recognize defective translation complexes in which ribosomes are stalled on
Bennett, Eric J, Lykke-Andersen, Jens
core   +2 more sources

A Single‐Enzyme Activated CRISPR‐Cas12a Nano System via Subtly Balanced dsDNA for Kinetic‐Gated UDG Detection and Spatiotemporal Cellular Imaging

Advanced Science, EarlyView.
Kinetic‐gated CRISPR–Cas12a activation enables single‐enzyme detection and spatiotemporal imaging of UDG ABSTRACT Uracil‐DNA glycosylase (UDG) is a key enzyme in base excision repair and an important biomarker for genomic stability and disease. In many reported sensing systems, uracil excision is coupled to signal generation through additional ...
Kejun Dong, Hao Hu, Haiyun Wang, Zijia Zheng, Shuangshuang Cheng, Wan Shu, Ruisi Liu, Xiaoyan Xin, Shiyi Huang, Dingchao Qian, Xianjin Xiao, Qiang Fu, Hongbo Wang   +12 more
wiley   +1 more source

Half a century after their discovery: Structural insights into exonuclease and annealase proteins catalyzing recombineering

Engineering Microbiology
Recombineering is an essential tool for molecular biologists, allowing for the facile and efficient manipulation of bacterial genomes directly in cells without the need for costly and laborious in vitro manipulations involving restriction enzymes.
Lucy J. Fitschen, Timothy P. Newing, Nikolas P. Johnston, Charles E. Bell, Gökhan Tolun   +4 more
doaj   +1 more source

Identification of PARN nuclease activity inhibitors by computational-based docking and high-throughput screening

Scientific Reports, 2023
Poly(A)-specific ribonuclease (PARN) is a 3′-exoribonuclease that removes poly(A) tails from the 3′ end of RNAs. PARN is known to deadenylate some ncRNAs, including hTR, Y RNAs, and some miRNAs and thereby enhance their stability by limiting the access ...
Thao Ngoc Huynh, Siddharth Shukla, Philip Reigan, Roy Parker   +3 more
doaj   +1 more source

Programmable RNA N6,2´‐O‐Dimethyladenosine Editing

Advanced Science, EarlyView.
ABSTRACT N6,2’‐O‐dimethyladenosine (m6Am) is a prevalent RNA modification located at the first transcribed nucleotide adjacent to the 5′ cap of mRNAs, where it has been implicated in gene regulation. However, the lack of methods for precise, transcript‐specific manipulation of m6Am has limited its functional dissection.
Yang Li, Xiangmin Tan, Yaran Liu, Yongquan He, Bo Yuan, Ping Wang, Guangzhi Ma, Mengzhe Guo, Jian Zhou, Qiang Sun   +9 more
wiley   +1 more source

A signal transmission strategy driven by gap-regulated exonuclease hydrolysis for hierarchical molecular networks

Communications Biology
Exonucleases serve as efficient tools for signal processing and play an important role in biochemical reactions. Here, we identify the mechanism of cooperative exonuclease hydrolysis, offering a method to regulate the cooperative hydrolysis driven by ...
Xin Liu, Xun Zhang, Shuang Cui, Shujuan Xu, Rongming Liu, Bin Wang, Xiaopeng Wei, Qiang Zhang   +7 more
doaj   +1 more source

Quinolones: a class of antimicrobial agents

Veterinární Medicína, 2001
The fluoroquinolones are a series of synthetic antibacterial agents that are used in the treatment of a variety of bacterial infections. These agents inhibit the DNA gyrase, abolishing its activity by interfering with the DNA-rejoining reaction.
G. Sárközy
doaj   +1 more source

Human Exonuclease 5 Is a Novel Sliding Exonuclease Required for Genome Stability [PDF]

Journal of Biological Chemistry, 2012
Previously, we characterized Saccharomyces cerevisiae exonuclease 5 (EXO5), which is required for mitochondrial genome maintenance. Here, we identify the human homolog (C1orf176; EXO5) that functions in the repair of nuclear DNA damage. Human EXO5 (hEXO5) contains an iron-sulfur cluster.
Justin L, Sparks, Rakesh, Kumar, Mayank, Singh, Marc S, Wold, Tej K, Pandita, Peter M, Burgers   +5 more
openaire   +2 more sources

Engineering a Virus‐Derived X Family DNA Polymerase FvPolX for de novo DNA Synthesis

Advanced Science, EarlyView.
In this study, a Faustovirus‐derived polymerase (FvPolX) is engineered based on structural and sequence analysis of PolX enzymes. The optimal mutants demonstrate substantially enhanced catalytic activity toward dNTPs and 3′‐ONH2‐dNTPs compared to the wild‐type enzyme.
Chengjie Zhang, Lei Du, Shengying Li
wiley   +1 more source