Results 81 to 90 of about 12,706 (198)

The exosome: A versatile RNA processing machine [PDF]

, 1998
A conserved complex of multiple 3′-to-5′ exonucleases has been discovered recently in eukaryotes.
Decker, Carolyn J
core   +1 more source

Time‐Resolved Effects of Cold Atmospheric Plasma on E. coli GW‐AmxH19 Transcriptome and Proteome in an Emulated Wastewater Environment

PROTEOMICS, EarlyView.
ABSTRACT Cold atmospheric plasma (CAP) is effective against a variety of microorganisms. Here, we described effects on an Escherichia coli strain isolated from hospital wastewater caused by treatment with physical plasma. E. coli GW‐AmxH19 was incubated in artificial wastewater and treated for 15 min with CAP.
Jörg Bernhardt, Dominik Schneider, Julia Rielicke, Susanne Sievers, Veronika Hahn, Mechthild Bömeke, Anja Poehlein, Dirk Albrecht, Juergen F. Kolb, Katharina Riedel, Rolf Daniel, Daniela Zühlke   +11 more
wiley   +1 more source

Carbon dots in electrochemiluminescence: Mechanisms, interface engineering, and bioanalytical applications

Responsive Materials, EarlyView.
Carbon dots are emerging as versatile electrochemiluminescent nanomaterials whose structure, surface states, and interfacial design jointly determine ECL pathways and performance. This review highlights how mechanistic understanding and materials engineering enable programmable CD‐ECL systems for bioanalysis, imaging, and future intelligent analytical ...
Yuhan Zhao, Zixuan Chen, Jun‐Jie Zhu
wiley   +1 more source

Programmable Assembly of Higher‐Order DNA Nanostructures from Microbial ssDNA Staple Libraries

Small, EarlyView.
A phage‐free, microbial platform produces programmable circular ssDNA libraries that are enzymatically converted into DNA origami staple pools. Rolling‐circle replication generates sequence‐complex ssDNA with >99% fidelity, enabling assembly of DNA tiles and tubules and supporting nanostructure formation even in crude cellular lysates.
Ming Hung Yen, Mallikarjuna Reddy Kesama, Yancheng Du, Jong Hyun Choi, Kevin V. Solomon   +4 more
wiley   +1 more source

3′-Terminated Overhangs Regulate DNA Double-Strand Break Processing in Escherichia coli

G3: Genes, Genomes, Genetics, 2017
Double-strand breaks (DSBs) are lethal DNA lesions, which are repaired by homologous recombination in Escherichia coli. To study DSB processing in vivo, we induced DSBs into the E. coli chromosome by γ-irradiation and measured chromosomal degradation. We
Edyta Đermić, Davor Zahradka, Dušica Vujaklija, Siniša Ivanković, Damir Đermić   +4 more
doaj   +1 more source

Peptide Fingerprinting of Single Protein Molecules Using In‐Plane Nanopore Sensors Integrated to an Extended Immobilized Nanoscale Enzymatic Reactor (eINER)

Small Methods, EarlyView.
The protein Time‐of‐Flight (P‐ToF) chip can peptide fingerprint a single protein molecule by subjecting it to a solid phase endopeptidase cleavage reaction in‐line with a combination of resistive pulse sensing of the resulting single peptides and machine‐learning aided identification.
Suresh Shivanka, Randy F. Espinal Cabrera, Maximillian Chibuike, Pramodi Vithanage, Indu A. Chandrasoma, Pubudu Premarathne, Matthew Verber, Steven A. Soper   +7 more
wiley   +1 more source

Structural and functional investigation of DinG containing a 3′–5′ exonuclease domain

mBio
Damage-inducible gene G (DinG), a bacterial homolog of SF2 helicase, has been extensively studied in Escherichia coli. However, the structural and functional characteristics of DinG homologs fused with an N-terminal 3′–5′ exonuclease domain, such as ...
Tianwen Gao, Wanshan Hao, Jin Gao, Yiyang Sun, Yukang Sun, Jieyu Yang, Kaiying Cheng   +6 more
doaj   +1 more source

Profiling and Validation of the Circular RNA Repertoire in Adult Murine Hearts

Genomics, Proteomics & Bioinformatics, 2016
For several decades, cardiovascular disease has been the leading cause of death throughout all countries. There is a strong genetic component to many disease subtypes (e.g., cardiomyopathy) and we are just beginning to understand the relevant genetic ...
Tobias Jakobi, Lisa F. Czaja-Hasse, Richard Reinhardt, Christoph Dieterich   +3 more
doaj   +1 more source

Evaluation of different types of end-capping modifications on the stability of oligonucleotides toward 3'- and 5'-exonucleases.

, 1999
Synthetic oligonucleotides are increasingly used because of their potential activity as regulators of gene expression. One of their major drawbacks is instability toward nucleases, in particular exonucleases.
Capobianco, Rauzi F, Pandolfi D, M
core   +1 more source

Electrochemical aptasensors for ultrasensitive multiplexed detection: Towards a new era of precision diagnostics and environmental monitoring

VIEW, EarlyView.
Electrochemical aptasensors are emerging as powerful platforms for precision diagnostics and environmental monitoring, owing to their high selectivity, programmability and compatibility with miniaturised systems. However, their broader application is constrained by limited signal intensity and challenges in reliable multiplexed detection.
Azadeh Abdi, Ngoan Thi Thao Nguyen, Riccardo Zulli, Jacopo Giaretta, Fariba Dehghani, Syamak Farajikhah   +5 more
wiley   +1 more source