Results 1 to 10 of about 8,258 (199)
Biosensors, 2023 Exonuclease III (Exo III) has been generally used as a double-stranded DNA (dsDNA)-specific exonuclease that does not degrade single-stranded DNA (ssDNA). Here, we demonstrate that Exo III at concentrations above 0.1 unit/μL can efficiently digest linear
Zhiqing Yang, Zixuan Guo
exaly +3 more sources
Kinetic Features of 3′-5′ Exonuclease Activity of Human AP-Endonuclease APE1
Molecules, 2018 Human apurinic/apyrimidinic (AP)-endonuclease APE1 is one of the key enzymes taking part in the repair of damage to DNA. The primary role of APE1 is the initiation of the repair of AP-sites by catalyzing the hydrolytic incision of the phosphodiester bond
Olga Fedorova +2 more
exaly +3 more sources
Opportunities and Challenges in Targeting the Proofreading Activity of SARS-CoV-2 Polymerase Complex
Molecules, 2022 Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of the COVID-19 pandemic. While the development of vaccines and the emergence of antiviral therapeutics is promising, alternative strategies to combat COVID-19 (and potential ...
Jerome Deval, Zachary A. Gurard-Levin
doaj +1 more source
Mutation Rates, Mutation Frequencies, and Proofreading-Repair Activities in RNA Virus Genetics
Viruses, 2021 The error rate displayed during template copying to produce viral RNA progeny is a biologically relevant parameter of the replication complexes of viruses.
Esteban Domingo +3 more
doaj +1 more source
Established and Emerging Methods for Protecting Linear DNA in Cell-Free Expression Systems
Methods and Protocols, 2023 Cell-free protein synthesis (CFPS) is a method utilized for producing proteins without the limits of cell viability. The plug-and-play utility of CFPS is a key advantage over traditional plasmid-based expression systems and is foundational to the ...
Trevor J. Fochtman, Javin P. Oza
doaj +1 more source
Identification of a Rho-Dependent Termination Site In Vivo Using Synthetic Small RNA
Microbiology Spectrum, 2023 Rho promotes Rho-dependent termination (RDT) at the Rho-dependent terminator, producing a variable-length region without secondary structure at the 3′ end of mRNA.
Xun Wang +4 more
doaj +1 more source
Identifying Structural Features of Nucleotide Analogues to Overcome SARS-CoV-2 Exonuclease Activity
Viruses, 2022 With the recent global spread of new SARS-CoV-2 variants, there remains an urgent need to develop effective and variant-resistant oral drugs. Recently, we reported in vitro results validating the use of combination drugs targeting both the SARS-CoV-2 RNA-
Xuanting Wang +7 more
doaj +1 more source
Biomolecules, 2020 Antisense oligonucleotides conjugated with boron clusters (B-ASOs) have been described as potential gene expression inhibitors and carriers of boron for boron neutron capture therapy (BNCT), providing a dual-action therapeutic platform. In this study, we
Damian Kaniowski +5 more
doaj +1 more source
Exonucleases: Degrading DNA to Deal with Genome Damage, Cell Death, Inflammation and Cancer
Cells, 2022 Although DNA degradation might seem an unwanted event, it is essential in many cellular processes that are key to maintaining genomic stability and cell and organism homeostasis.
Joan Manils +2 more
doaj +1 more source
PLoS Pathogens, 2023 The arenavirus nucleoprotein (NP) plays an important role in the virus’ ability to block interferon (IFN) production, and its exonuclease function appears to contribute to this activity.
Patrick Bohn +6 more
doaj +2 more sources