Results 11 to 20 of about 8,258 (199)
Inhibition of Arenaviridae nucleoprotein exonuclease by bisphosphonate
IUCrJ, 2022 Arenaviruses are emerging enveloped negative-sense RNA viruses that cause neurological and hemorrhagic diseases in humans. Currently, no FDA-approved vaccine or therapeutic agent is available except for ribavirin, which must be administered early during ...
Thi Hong Van Nguyen +5 more
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Exonuclease editor promotes precision of gene editing in mammalian cells
BMC BiologyBackground Many efforts have been made to improve the precision of Cas9-mediated gene editing through increasing knock-in efficiency and decreasing byproducts, which proved to be challenging.
Hui Shi +9 more
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Structural basis of Ku-mediated activation of WRN exonuclease activity
Nature CommunicationsWerner (WRN) is the only human RecQ helicase family member with DNA exonuclease activity. WRN promotes genome stability through its functions in DNA replication, repair and telomere maintenance, the deficiency of which presents clinically as Werner ...
Sayma Zahid +18 more
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Sae2 controls Mre11 endo- and exonuclease activities by different mechanisms
Nature CommunicationsDNA double-strand breaks (DSBs) must be repaired to ensure cell survival and genomic integrity. In yeast, the Mre11-Rad50-Xrs2 complex (MRX) collaborates with Sae2 to initiate DSB repair. Sae2 stimulates two MRX nuclease activities, endonuclease and 3’−5’
Tomoki Tamai +7 more
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Proofreading deficiency of Pol I increases the levels of spontaneous rpoB mutations in E. coli [PDF]
, 2011 The fidelity role of DNA polymerase I in chromosomal DNA replication in E. coli was investigated using the rpoB forward target. These experiments indicated that in a strain carrying a proofreading-exonuclease-defective form of Pol I (polAexo mutant) the ...
Jonczyk, Piotr +7 more
core +2 more sources
Biosensors, 2023 In this paper, we developed a sensitive, label-free and facile fluorescent strategy for detecting streptavidin (SA) based on double-hairpin DNA-templated copper nanoparticles (CuNPs) and terminal protection of small molecule-linked DNA. Herein, a special
Qiangsheng Xiao +5 more
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TREX1 as a Novel Immunotherapeutic Target
Frontiers in Immunology, 2021 Mutations in the TREX1 3’ → 5’ exonuclease are associated with a spectrum of autoimmune disease phenotypes in humans and mice. Failure to degrade DNA activates the cGAS-STING DNA-sensing pathway signaling a type-I interferon (IFN) response that ...
Wayne O. Hemphill +6 more
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An efficient early‐pooling protocol for environmental DNA metabarcoding
Environmental DNA, 2022 Environmental DNA (eDNA) metabarcoding, a method that applies high‐throughput sequencing and universal primer sets to eDNA analysis, has been a promising approach for efficient, comprehensive biodiversity monitoring.
Masayuki Ushio +4 more
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Brothers in Arms: Structure, Assembly and Function of Arenaviridae Nucleoprotein
Viruses, 2020 Arenaviridae is a family of viruses harbouring important emerging pathogens belonging to the Bunyavirales order. Like in other segmented negative strand RNA viruses, the nucleoprotein (NP) is a major actor of the viral life cycle being both (i) the ...
Nicolas Papageorgiou +8 more
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Pharmaceutics, 2021 The adult Göttingen Minipig is an acknowledged model for safety assessment of antisense oligonucleotide (ASO) drugs developed for adult indications.
Allan Valenzuela +13 more
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