Results 31 to 40 of about 8,258 (199)
Frontiers in Microbiology, 2023 In Phi29-α–hemolysin (α-HL) nanopore sequencing systems, a strong electrochemical signal is dependent on a high concentration of salt. However, high salt concentrations adversely affect polymerase activity.
Yaping Sun +10 more
doaj +1 more source
CELiD-GFP DNA sensitivity to exonuclease I and exonuclease III.
, 2013 E. coli exonuclease III (ExoIII) removes nucleotides processively (3′ –>5′) from DNA initiating at a 3′-OH of either blunt-ended or 5′ protruding duplex DNA. E. coli exonuclease I (ExoI) degrades single-stranded DNA processively in a 3′ to 5′ direction. (
Richard H. Smith (440436) +9 more
core +1 more source
p53 Protein Exhibits 3′-to-5′ Exonuclease Activity [PDF]
, 1996 Highly purified p53 protein from different sources was able to degrade DNA with a 3′-to-5′ polarity, yielding deoxynucleoside monophosphates as reaction products.
Janus, Friedemann +5 more
core +1 more source
Biochemical Characterization of an Exonuclease from Arabidopsis thaliana Reveals Similarities to the DNA Exonuclease of the Human Werner Syndrome Protein [PDF]
, 2010 The human Werner syndrome protein (hWRN-p) possessing DNA helicase and exonuclease activities is essential for genome stability. Plants have no homologue of this bifunctional protein, but surprisingly the Arabidopsis genome contains a small open reading ...
Puchta, H., Hartung, F., Plchova, H.
core +1 more source
Construction of TCRV nucleoprotein NP exonuclease mutants.
, 2023 (A) Structural model of the TCRV NP exonuclease active site. A homology model of the TCRV NP C-terminal domain (PDB: 4GVE) based on the structure of the LASV NP CTD bound to dsRNA (PDB: 4VFU) is shown. Residues critical for exonuclease activity are shown
Patrick Bohn (14334371) +6 more
core +1 more source
FEBS Letters, EarlyView.How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley +1 more source
, 2014 Members of the RecQ family of helicases are known for their roles in DNA repair, replication, and recombination. Mutations in the human RecQ helicases, WRN and BLM, cause Werner and Bloom syndromes, which are diseases characterized by genome instability ...
Bolterstein, Elyse +3 more
core +1 more source
Exonuclease 1-dependent and independent mismatch repair [PDF]
, 2015 DNA mismatch repair (MMR) acts to repair mispaired bases resulting from misincorporation errors during DNA replication and also recognizes mispaired bases in recombination (HR) intermediates.
Putnam, Christopher D +2 more
core +1 more source
Molecular Oncology, EarlyView.Isocitrate dehydrogenase 1 (IDH1) mutations are highly recurrent in multiple human cancer types, including cholangiocarcinoma and glioma. IDH1 R132C is the most common IDH1 mutation in cholangiocarcinoma and likely arises from APOBEC3A‐ or APOBEC3B‐mediated deamination.
Kelly E. Butler +3 more
wiley +1 more source
Angewandte Chemie, EarlyView.A new exponential RCA (E‐RCA) system was developed and coupled to a toehold system for release of a bridging DNA, allowing for lateral flow detection. The E‐RCA method uses a secondary primer (P2) that binds RCA products (RCAP) to generate extended P2 products that can re‐prime a partially self‐complementary circular template, generating a complete ...
Amal Mathai +3 more
wiley +2 more sources