Results 51 to 60 of about 8,258 (199)
Fidelity of a Bacterial DNA Polymerase in Microgravity, a Model for Human Health in Space
Frontiers in Cell and Developmental Biology, 2021 Long-term space missions will expose crew members, their cells as well as their microbiomes to prolonged periods of microgravity and ionizing radiation, environmental stressors for which almost no earth-based organisms have evolved to survive.
Aaron H Rosenstein, Virginia K Walker
doaj +1 more source
Iterative Selection of DNA Nanostructures for Cellular Uptake
Advanced Materials, EarlyView.DNA nanostructures are promising cell targeting delivery vehicles for therapeutics, but the targeting behavior is not fully understood. In this study, libraries of DNA nanostructures that can be amplified and sequenced are combined with cellular uptake as a selection pressure to iteratively refine DNA structures taken up in cells to better understand ...
Anjali Rajwar +4 more
wiley +1 more source
mBioSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) non-structural protein 14 (Nsp14) possesses an N-terminal exonuclease (ExoN) domain that provides a proofreading function for the viral RNA-dependent RNA polymerase and a C-terminal N7 ...
Naveen Thakur +3 more
doaj +1 more source
, 2009 MutS and UvrD proteins individually stimulate Escherichia coli exonuclease VII activity on blunt-ended short duplex DNA substrates. Stimulation by both proteins is ATP-dependent but not mismatch-specific and is not accompanied by apparent strand ...
Noothi, Sunil K. +2 more
core +1 more source
Exonuclease I Hydrolyzes DNA with a Distribution of Rates [PDF]
, 2005 We report heterogeneity in the time necessary for Exonuclease I to hydrolyze identical DNA fragments. A real-time fluorescence method measured the time required by molecules of Exonuclease I to hydrolyze single-stranded DNA that was synthesized to have ...
Keller, Richard A. +3 more
core +1 more source
Attomolar Detection of HIV‐1 With Label‐Free RCA‐rCRISPR on Smartphone
Advanced Science, EarlyView.A new diagnostic strategy integrating rolling circle amplification (RCA) with a plasmid reporter–based ratiometric CRISPR (rCRISPR) system was developed to detect unfragmented HIV genomes with single‐digit attomolar sensitivity. The method quantifies targets using the plasmid relaxation ratio and employs a smartphone‐based mini‐gel device to enable ...
Noor Mohammad +12 more
wiley +1 more source
NMR applications to find and progress TREX1 binders
Journal of Magnetic Resonance Open, 2022 Inhibition of the exonuclease activity of Three-prime Repair EXonuclease 1 (TREX1) has emerged as a potential novel immunotherapeutic strategy. Herein we describe our screening approach to find initial low-affinity fragment hits for TREX1 from our 19F ...
Leonhard Geist +7 more
doaj +1 more source
Nucleotide Specificity versus Complex Heterogeneity in Exonuclease Activity Measurements [PDF]
, 2006 A recent publication reported on measurements of Exonuclease I activity using a real-time fluorescence method that measures the time required by molecules of Exonuclease I to hydrolyze single-stranded DNA that was synthesized to have two fluorescently ...
Enderlein, Jörg, Enderlein, J.
core +1 more source
Advanced Science, EarlyView.A novel epitranscriptomic mechanism in rheumatoid arthritis is uncovered: NSUN2 promotes disease via m5C‐dependent stabilization of ICMT mRNA, fueling the migration and invasion of pathogenic RA FLS. Targeting this axis with engineered nanoparticles (Ce/SAA NPs) effectively inhibits disease progression, presenting a precise therapeutic strategy ...
Ruiru Li +15 more
wiley +1 more source
The APE2 Exonuclease Is a Client of the Hsp70–Hsp90 Axis in Yeast and Mammalian Cells
Biomolecules, 2022 Molecular chaperones such as Hsp70 and Hsp90 help fold and activate proteins in important signal transduction pathways that include DNA damage response (DDR).
Siddhi Omkar +4 more
doaj +1 more source