Results 61 to 70 of about 8,258 (199)

circZNF148 Drives Glucose Metabolism Reprogramming to Enhance Metastasis and Immune Evasion via HK1 Stabilization in Triple‐Negative Breast Cancer

Advanced Science, EarlyView.
CircZNF148 stabilizes HK1 through deubiquitinase recruitment, thereby enhancing glycolysis and lactate production. Elevated lactate promotes PD‑L1 lactylation and membrane accumulation while suppressing CD8+ T‐cell cytotoxicity, collectively facilitating immune evasion and malignant progression in TNBC.
Yuhan Jin, Wenjing Zhao, Lei Wang, Jianing Wang, Keying Zhao, Dan Luo, Yifei Wang, Yuxia Yang, Jingze Yang, Yaming Li, Tong Chen, Dianwen Han, Zekun Wang, Xiaoyan Li, Bing Chen, Lijuan Wang, Yiran Liang, Qifeng Yang   +17 more
wiley   +1 more source

Biochemical Characterization of an Exonuclease from Arabidopsis thaliana Reveals Similarities to the DNA Exonuclease of the Human Werner Syndrome Protein*

, 2003
The human Werner syndrome protein (hWRN-p) possessing DNA helicase and exonuclease activities is essential for genome stability. Plants have no homologue of this bifunctional protein, but surprisingly the Arabidopsis genome contains a small open ...
Plchova, Helena, Puchta, Holger, Hartung, Frank   +2 more
core   +1 more source

Rapid Exonuclease‐Based De Novo Sequencing Strategy for Long Oligonucleotides via Liquid‐Phase Ion Trap Coupled With Mass Spectrometry

Advanced Science, EarlyView.
A liquid‐phase ion trap (LPIT) coupled with ESI‐MS enables rapid, high‐fidelity de novo sequencing of long oligonucleotides in ∼30 min. By applying an electric field to dissociate metal adducts in solution, LPIT delivers clean spectra, superior mass accuracy, and full sequence coverage, surpassing MALDI‐TOF and LC‐MS.
Song Lu, Jie Hong, Xinyan Fu, Di Wang, Yunhua Gao, Wei Xu   +5 more
wiley   +1 more source

Molecular snapshots of APE1 proofreading mismatches and removing DNA damage

Nature Communications, 2018
The essential DNA repair enzyme apurinic/apyrimidinic endonuclease 1 (APE1) has both endonuclease and exonuclease activities. Here, the authors present DNA bound human APE1 crystal structures which give insights into its exonuclease mechanism.
Amy M. Whitaker, Tony S. Flynn, Bret D. Freudenthal   +2 more
doaj   +1 more source

Restoration of m6A‐Modified circPSMB1 Suppresses Chordoma Progression and Enables Local Nanotherapeutic Intervention

Advanced Science, EarlyView.
An m6A‐modified circRNA, circPSMB1, is identified as a tumor suppressor in chordoma. METTL3‐dependent m6A promotes circPSMB1 interaction with HNRNPA2B1, limiting c‐MYC expression and malignant progression. An injectable HMnO2‐based nanohydrogel enables local circPSMB1 delivery and tumor microenvironment‐responsive therapy, offering a promising strategy
Yingchuang Tang, Liang Qiu, Xingbang Ruan, Yihan Shi, Ye Wang, Hanwen Li, Kai Zhang, Kangwu Chen   +7 more
wiley   +1 more source

Distinct Cleavage Trajectories Mediated by DNA and RNA Substrate in Argonaute From Methanocaldococcus Fervens

Advanced Science, EarlyView.
This study characterizes MfAgo, an enzyme exhibiting highly efficient DNA‐guided DNA and RNA cleavage capability. Precise RNA cleavage strictly requires dimerization to stabilize the PIWI‐loop region, while its robust DNA cleavage functions in a dimerization‐independent manner.
Fuyong Huang, Taiyu Chen, Meng Fang, Qinmiao Duanmu, Kangle Mu, Yuqi Bi, Siwei Xu, Xin Tao, Jiening Wang, Shan Wu   +9 more
wiley   +1 more source

Structural and functional investigation of DinG containing a 3′–5′ exonuclease domain

mBio
Damage-inducible gene G (DinG), a bacterial homolog of SF2 helicase, has been extensively studied in Escherichia coli. However, the structural and functional characteristics of DinG homologs fused with an N-terminal 3′–5′ exonuclease domain, such as ...
Tianwen Gao, Wanshan Hao, Jin Gao, Yiyang Sun, Yukang Sun, Jieyu Yang, Kaiying Cheng   +6 more
doaj   +1 more source

Exonuclease-enhanced prime editors [PDF]

Prime editing (PE) is a powerful gene-editing technique based on targeted gRNA-templated reverse transcription and integration of the de novo synthesized single-stranded DNA. To circumvent one of the main bottlenecks of the method, the competition of the
Geilenkeuser, Julian, Siebenhaar, Annika, Truong, Dong-Jiunn Jeffery, Westmeyer, Gil Gregor, Wendel, Stephanie Victoria, Živanić, Milica, Armbrust, Niklas, Santl, Tobias Heinrich, Gruber, Christoph, Clemens, Julius, Phlairaharn, Teeradon, Hildegard Binder, Eva Maria   +11 more
core   +1 more source

Human DNA polymerase delta requires an iron–sulfur cluster for high-fidelity DNA synthesis

Life Science Alliance, 2019
The iron–sulfur cluster in human DNA polymerase delta has an impact on DNA polymerase and exonuclease activities and can hence influence the fidelity of DNA synthesis. Replication of eukaryotic genomes relies on the family B DNA polymerases Pol α, Pol δ,
Stanislaw K. Jozwiakowski, Sandra Kummer, Kerstin Gari   +2 more
doaj   +1 more source

The effects of AICAR and rapamycin on mitochondrial function in immortalized mitochondrial DNA mutator murine embryonic fibroblasts

Biology Open, 2018
Mitochondrial DNA mutations accumulate with age and may play a role in stem cell aging as suggested by the premature aging phenotype of mitochondrial DNA polymerase gamma (POLG) exonuclease-deficient mice.
Vedad Delic, Kenyaria Noble, Sandra Zivkovic, Tam-Anh Phan, Christian Reynes, Yumeng Zhang, Oluwakemi Phillips, Charles Claybaker, Yen Ta, Vinh B. Dinh, Josean Cruz, Tomas A. Prolla, Patrick C. Bradshaw   +12 more
doaj   +1 more source