Results 41 to 50 of about 34,514 (185)
Targeted genome modifications in soybean with CRISPR/Cas9 [PDF]
, 2015 Background: The ability to selectively alter genomic DNA sequences in vivo is a powerful tool for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA sequences in a number of organisms.
Jacobs, Thomas +3 more
core +2 more sources
Targeted mutagenesis using CRISPR-Cas9 in the chelicerate herbivore Tetranychus urticae [PDF]
, 2020 The use of CRISPR-Cas9 has revolutionized functional genetic work in many organisms, including more and more insect species. However, successful gene editing or genetic transformation has not yet been reported for chelicerates, the second largest group ...
Dermauw, Wannes +5 more
core +1 more source
Physical Model of the Immune Response of Bacteria Against Bacteriophage Through the Adaptive CRISPR-Cas Immune System [PDF]
, 2013 Bacteria and archaea have evolved an adaptive, heritable immune system that recognizes and protects against viruses or plasmids. This system, known as the CRISPR-Cas system, allows the host to recognize and incorporate short foreign DNA or RNA sequences,
Barrick, Jeffrey E. +3 more
core +1 more source
Evaluation of Engineered CRISPR-Cas-Mediated Systems for Site-Specific RNA Editing
Cell Reports, 2020 Summary: Site-directed RNA editing approaches offer great potential to correct genetic mutations in somatic cells while avoiding permanent off-target genomic edits.
Ryan J. Marina +4 more
doaj +1 more source
mSphere, 2023 The rapid development of CRISPR/CRISPR-associated (Cas) systems has revolutionized the ability to produce genetic mutations in a desired locus, particularly in organisms with low rates of homologous recombination.
Chad A. Rappleye
doaj +1 more source
CRISPR/Cas-based screening of long non-coding RNAs (lncRNAs) in macrophages with an NF-κB reporter. [PDF]
, 2017 The innate immune system protects against infections by initiating an inducible inflammatory response. NF-κB is one of the critical transcription factors controlling this complex response, but some aspects of its regulation remain unclear.
Carpenter, Susan +8 more
core +1 more source
Expanding the CRISPR toolbox in Culicine mosquitoes: in vitro validation of Pol III promoters [PDF]
, 2020 CRISPR–Cas9-based “gene drive” technologies have been proposed as a novel and effective means of controlling human diseases vectored by mosquitoes. However, more complex designs than those demonstrated to date—and an expanded molecular toolbox with which
Alphey, Luke +6 more
core +1 more source
CRISPR Content Correlates with the Pathogenic Potential of Escherichia coli. [PDF]
PLoS ONE, 2015 Guide RNA molecules (crRNA) produced from clustered regularly interspaced short palindromic repeat (CRISPR) arrays, altogether with effector proteins (Cas) encoded by cognate cas (CRISPR associated) genes, mount an interference mechanism (CRISPR-Cas ...
Enriqueta García-Gutiérrez +4 more
doaj +1 more source
The physicist's guide to one of biotechnology's hottest new topics: CRISPR-Cas
, 2018 Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) constitute a multi-functional, constantly evolving immune system in bacteria and archaea cells.
Bonomo, Melia E., Deem, Michael W.
core +1 more source
Microbial Biotechnology, 2023 Non‐coding RNAs (crRNAs) produced from clustered regularly interspaced short palindromic repeats (CRISPR) loci and CRISPR‐associated (Cas) proteins of the prokaryotic CRISPR‐Cas systems form complexes that interfere with the spread of transmissible ...
Belen Esquerra‐Ruvira +5 more
doaj +1 more source