Results 41 to 50 of about 21,319 (117)

Anti-CRISPR AcrIIA5 Potently Inhibits All Cas9 Homologs Used for Genome Editing

Cell Reports, 2019
Summary: CRISPR-Cas9 systems provide powerful tools for genome editing. However, optimal employment of this technology will require control of Cas9 activity so that the timing, tissue specificity, and accuracy of editing may be precisely modulated.
Bianca Garcia, Jooyoung Lee, Alireza Edraki, Yurima Hidalgo-Reyes, Steven Erwood, Aamir Mir, Chantel N. Trost, Uri Seroussi, Sabrina Y. Stanley, Ronald D. Cohn, Julie M. Claycomb, Erik J. Sontheimer, Karen L. Maxwell, Alan R. Davidson   +13 more
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DNA targeting by compact Cas9d and its resurrected ancestor

Nature Communications
Type II CRISPR endonucleases are widely used programmable genome editing tools. Recently, CRISPR-Cas systems with highly compact nucleases have been discovered, including Cas9d (a type II-D nuclease).
Rodrigo Fregoso Ocampo, Jack P. K. Bravo, Tyler L. Dangerfield, Isabel Nocedal, Samatar A. Jirde, Lisa M. Alexander, Nicole C. Thomas, Anjali Das, Sarah Nielson, Kenneth A. Johnson, Christopher T. Brown, Cristina N. Butterfield, Daniela S. A. Goltsman, David W. Taylor   +13 more
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Dramatic Improvement of CRISPR/Cas9 Editing in Candida albicans by Increased Single Guide RNA Expression

mSphere, 2017
The clustered regularly interspaced short palindromic repeat system with CRISPR-associated protein 9 nuclease (CRISPR/Cas9) has emerged as a versatile tool for genome editing in Candida albicans.
Henry Ng, Neta Dean
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AcrIIA19 binds to the WED domain and inhibits various Cas9 orthologs at multiple stages

Communications Biology
Anti-CRISPR (Acr) proteins are natural inhibitors of clustered regularly interspaced short palindromic repeat (CRISPR)–CRISPR-associated protein (Cas) systems, providing valuable tools for regulating genome editing. Here, we present the crystal structure
Gi Eob Kim, So Yeon Lee, Yong Jun Kang, Hyo Bin Jin, Hyun Ho Park   +4 more
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Repurposing CRISPR-Cas13 systems for robust mRNA trans-splicing

Nature Communications
Type VI CRISPR enzymes have been developed as programmable RNA-guided Cas proteins for eukaryotic RNA editing. Notably, Cas13 has been utilized for site-targeted single base edits, demethylation, RNA cleavage or knockdown and alternative splicing ...
David N. Fiflis, Nicolas A. Rey, Harshitha Venugopal-Lavanya, Beatrice Sewell, Aaron Mitchell-Dick, Katie N. Clements, Sydney Milo, Abigail R. Benkert, Alan Rosales, Sophia Fergione, Aravind Asokan   +10 more
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CRISPR/Cas as a tool to overcome drug resistance in cancer: From challenge to opportunity

Molecular and Cellular Probes
Drug resistance remains a significant challenge in cancer therapy, often resulting in treatment failure, tumor progression, and metastasis. The underlying resistance mechanisms—including genetic mutations, epigenetic alterations, and modifications in ...
Bashdar Mahmud Hussen, Snur Rasool Abdullah, Hazha Jamal Hidayat, Mark C. Glassy, Arash Safarzadeh, Alireza Komaki, Majid Samsami, Mohammad Taheri   +7 more
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TracrRNA reprogramming enables direct PAM-independent detection of RNA with diverse DNA-targeting Cas12 nucleases

Nature Communications
Many CRISPR-Cas immune systems generate guide (g)RNAs using trans-activating CRISPR RNAs (tracrRNAs). Recent work revealed that Cas9 tracrRNAs could be reprogrammed to convert any RNA-of-interest into a gRNA, linking the RNA’s presence to Cas9-mediated ...
Chunlei Jiao, Natalia L. Peeck, Jiaqi Yu, Mohammad Ghaem Maghami, Sarah Kono, Daphne Collias, Sandra L. Martinez Diaz, Rachael Larose, Chase L. Beisel   +8 more
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CRISPR/Cas9 technology in tumor research and drug development application progress and future prospects

Frontiers in Pharmacology
The CRISPR/Cas9 system is an acquired immune defense mechanism that has evolved in bacteria and archaea to protect against viral and plasmid attacks. It consists of regularly spaced clusters of short palindromic repeats (CRISPR) and CRISPR-associated ...
Han Han, Xiaoyan Sun, Xiaoyun Guo, Jiaxin Wen, Xiaoming Zhao, Weiqiang Zhou   +5 more
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Testing multiplexed anti-ASFV CRISPR-Cas9 in reducing African swine fever virus

Microbiology Spectrum
African swine fever (ASF) is a highly fatal viral disease that poses a significant threat to domestic pigs and wild boars globally. In our study, we aimed to explore the potential of a multiplexed CRISPR-Cas system in suppressing ASFV replication and ...
Zezhong Zheng, Lei Xu, Yangbin Gao, Hongwei Dou, Yixuan Zhou, Xu Feng, Xiangjun He, Zhen Tian, Lingling Song, Guolong Mo, Jiapan Hu, Hongye Zhao, Hongjiang Wei, George M. Church, Luhan Yang   +14 more
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Unveiling the invisible genomic dynamics

Experimental and Molecular Medicine
CRISPR-based imaging technologies have emerged as powerful tools for visualizing specific genomic loci, providing groundbreaking insights into chromatin structure and dynamics. Here, in this Review, we discuss the development and recent advances in these
Jiwoong Kwon, Yeji Park, Taekjip Ha
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