Results 61 to 70 of about 22,577 (244)

An Epigenetic Fate Converter Based on Nuclei‐Targeting Lipid Nanoparticles Drives Neuronal Programming of Stem Cells for Spinal Cord Repair

Advanced Functional Materials, EarlyView.
Schematic illustration of LNP‐MPG nuclei‐targeting delivery of HMW‐FGF2 promoting histone acetylation to regulate the fate of DPSCs and treat spinal cord injury. LNPs components include pHMW‐FGF2 plasmid, DSPC, Dlin‐MC3‐DMA, cholesterol, and PEG2000, and are modified with MPG to form HMW‐FGF2@LNP‐MPG (HLM). HLM nuclei‐targets DPSCs to deliver HMW‐FGF2,
Heng Zhou, Qiang Qin, Xiqin Li, Hanyu Ding, Chun Xu, Yan He, Qingsong Ye   +6 more
wiley   +1 more source

Cytosolic CRISPR RNAs for efficient application of RNA-targeting CRISPR-Cas systems

EMBO Reports
Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) technologies have evolved rapidly over the past decade with the continuous discovery of new Cas systems.
Ezra C K Cheng, Joe K C Lam, S Chul Kwon
doaj   +1 more source

CRISPR/Cas13d-Mediated Microbial RNA Knockdown

Frontiers in Bioengineering and Biotechnology, 2020
RNA-guided and RNA-targeting type IV-D CRISPR/Cas systems (CRISPR/Cas13d) have recently been identified and employed for efficient and specific RNA knockdown in mammalian and plant cells.
Kun Zhang, Kun Zhang, Zhihui Zhang, Zhihui Zhang, Jianan Kang, Jiuzhou Chen, Jiao Liu, Ning Gao, Ning Gao, Liwen Fan, Liwen Fan, Ping Zheng, Ping Zheng, Ping Zheng, Yu Wang, Yu Wang, Jibin Sun, Jibin Sun   +17 more
doaj   +1 more source

Microfluidic Nano‐Assembly of Red‐Blood‐Cell (RBC) Lipids and Components for Engineering Extracellular Vesicles

Advanced Healthcare Materials, EarlyView.
Engineered red blood cell‐derived extracellular vesicles (eRBCEVs) are synthesized via controlled microfluidic assembly from native RBC lipids, enabling tunable encapsulation of proteins, nucleic acids, nanoparticles, and viral vectors. The platform demonstrates reproducible nanoscale architecture, preserved membrane composition, and functional cargo ...
Chiranth K. Nagaraj, Xilal Y. Rima, Kim T. Nguyen, Courtney Culkins, Nana Boateng, Jacob Doon‐Ralls, Alejandro Bresolin, Xin Huang, Hong Li, Vahedi Amid, Ajeet Pal Singh, Dharti Shantaram, Anastasiia Amari, Mangesh D. Hade, Nicholas R. Merriam, Zachary D. Schultz, Scott Q. Harper, Nizar Y. Saad, Setty M. Magaña, Willa A. Hsueh, Rachel E. Kopec, Blaise R. Kimmel, Andre F. Palmer, Eduardo Reátegui   +23 more
wiley   +1 more source

CRISPR-Cas9 In Situ engineering of subtilisin E in Bacillus subtilis.

PLoS ONE, 2019
CRISPR-Cas systems have become widely used across all fields of biology as a genome engineering tool. With its recent demonstration in the Gram positive industrial workhorse Bacillus subtilis, this tool has become an attractive option for rapid ...
Marcus A Price, Rita Cruz, Scott Baxter, Franck Escalettes, Susan J Rosser   +4 more
doaj   +1 more source

Advanced Materials for Biologics Delivery to Brain Tumors

Advanced Materials, EarlyView.
Material innovation is central to unlocking the therapeutic potential of biologics against many central nervous system diseases, including brain cancer. By engineering carriers with controlled transport, targeting, and release properties, advanced materials can overcome the blood–brain barrier and tumor microenvironment, improving the delivery of ...
Yuran Feng, Yuxue Cao, Maria Kavallaris, Taskeen Janjua, Amirali Popat   +4 more
wiley   +1 more source

CRISPR-Cas as a Chemically Programmable System: Advances in Modulation and Delivery

Chemistry Proceedings
CRISPR-Cas systems have transformed genome engineering with their exceptional precision, programmability, and affordability. Although they originate from microbial defense mechanisms, expanding their use, especially in therapeutics, requires a chemically
Yukti Sabikhi, Anshika Singh, Chhavi Dudeja, Sameen Masroor, Richa Gupta   +4 more
doaj   +1 more source

Ionic Gating of Liquid‐Crystal‐Like Pteridine Assemblies Enables Tunable Light Scattering

Advanced Materials, EarlyView.
Tunable optical properties emerge through developmentally regulated ionic gating, which transforms disordered, ultraviolet (UV)‐absorbing organelles into concentrically ordered, liquid‐crystal‐like light scatterers. By integrating analyses of pteridine composition, potassium‐dependent assembly, hierarchical ultrastructure, and optical behavior, the ...
Sourabh Bera, Zohar Eyal, Yael Noy, Yuval Barzilay, Siddharth Sahoo, Srikant Moharana, Avi Baram, Tali Lerer‐Goldshtein, Neta Varsano, Bar Bader, Lothar Houben, Uwe Heinig, Iddo Pinkas, Moshe Goldsmith, Tamar Unger, Shira Albeck, Andrea Sorrentino, Maxim Itkin, Sergey Malitsky, Ziv Porat, Ido Hadar, Venkata Jayasurya Yallapragada, Dvir Gur   +22 more
wiley   +1 more source

Bidirectional Promoter-Based CRISPR-Cas9 Systems for Plant Genome Editing

Frontiers in Plant Science, 2019
CRISPR-Cas systems can be expressed in multiple ways, with different capabilities regarding tissue-specific expression, efficiency, and expression levels. Thus far, three expression strategies have been demonstrated in plants: mixed dual promoter systems,
Qiurong Ren, Zhaohui Zhong, Yan Wang, Qi You, Qi You, Qian Li, Mingzhu Yuan, Yao He, Caiyan Qi, Xu Tang, Xuelian Zheng, Tao Zhang, Tao Zhang, Yiping Qi, Yiping Qi, Yong Zhang, Yong Zhang   +16 more
doaj   +1 more source

Microfluidic Droplet Plate Preserving Single Human Induced Pluripotent Stem Cell‐Derived Cardiomyocytes Morphology and Function

Advanced Materials Technologies, EarlyView.
We introduce a novel method combining microfluidics, optogenetics, and human induced pluripotent stem cell (iPSC) technology to encapsulate single iPSC‐derived cardiomyocytes (iPSC‐CMs) in an optimal environment for functional characterization. This method enables direct genotype‐phenotype correlation for genetic studies and high‐throughput screening ...
Xiao‐Ting Wang, Rachel P. White, Yashasvi Tharani, Michael A. Finocchiaro, Leona M. Ripple, Maksymilian Prondzynski, Tania Konry, Vassilios J. Bezzerides   +7 more
wiley   +1 more source