Results 71 to 80 of about 34,514 (185)

Genomic and proteomic biases inform metabolic engineering strategies for anaerobic fungi. [PDF]

, 2020
Anaerobic fungi (Neocallimastigomycota) are emerging non-model hosts for biotechnology due to their wealth of biomass-degrading enzymes, yet tools to engineer these fungi have not yet been established.
Albà, Arazoe, Atasoglu, Bach, Beckham, Bezanson, Birdsell, Boch, Bonugli-Santos, Brownlee, Calkins, Camacho, Camiolo, Carlson, Chan, Chen, Cheng, Cheng, Chokhawala, Coker, Deshpande, Diener, Dollhofer, Duarte, Durand, Duret, Fondon, Galtier, Gasiunas, Gentzsch, Gerngross, Glass, Glémin, Greene, Grigoriev, Haitjema, Haitjema, Hamilton, Hanafy, Hartfield, Henske, Hershberg, Hildebrand, Hull, Jiang, Karlin, Kiktev, Kleinstiver, Kleinstiver, Knauer, Knight, Kuyper, Leberer, Li, Liggenstoffer, Liu, Magee, Mertens, Meunier, Morrison, Murphy, Nicholson, Nieuwenhuis, Nørholm, Orpin, Oyola, O’Malley, Podolsky, Raymond, Reichenberger, Ropars, Sadhu, Sammond, Sekowska, Seppälä, Seppälä, Solieri, Solomon, Sonan, Staben, Steensels, Steensels, Sukumaran, Theodorou, UniProt: a worldwide hub of protein knowledge, Videvall, Wang, Wang, Wright, Wu, Ximenes, Youssef, Zetsche   +92 more
core   +1 more source

Revolutionizing CRISPR technology with artificial intelligence

Experimental and Molecular Medicine
Genome engineering has made remarkable strides, evolving from DNA-binding proteins such as zinc fingers and transcription activator-like effectors to CRISPR–Cas systems. CRISPR technology has revolutionized the field through its simplicity and ability to
Min-gyeong Kim, Min-ji Go, Seung-Hun Kang, Soo-hwan Jeong, Kayeong Lim   +4 more
doaj   +1 more source

Bidirectional Promoter-Based CRISPR-Cas9 Systems for Plant Genome Editing

Frontiers in Plant Science, 2019
CRISPR-Cas systems can be expressed in multiple ways, with different capabilities regarding tissue-specific expression, efficiency, and expression levels. Thus far, three expression strategies have been demonstrated in plants: mixed dual promoter systems,
Qiurong Ren, Zhaohui Zhong, Yan Wang, Qi You, Qi You, Qian Li, Mingzhu Yuan, Yao He, Caiyan Qi, Xu Tang, Xuelian Zheng, Tao Zhang, Tao Zhang, Yiping Qi, Yiping Qi, Yong Zhang, Yong Zhang   +16 more
doaj   +1 more source

Cytosolic CRISPR RNAs for efficient application of RNA-targeting CRISPR-Cas systems

EMBO Reports
Clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) technologies have evolved rapidly over the past decade with the continuous discovery of new Cas systems.
Ezra C K Cheng, Joe K C Lam, S Chul Kwon
doaj   +1 more source

Direct ENIT: An easy and reliable tool for gRNA efficacy verification by tracking induced chromosomal translocation

MethodsX, 2020
CRISPR/Cas systems (Clustered regularly interspaced palindromic repeats / CRISPR-associated) are rapidly becoming a commonplace and popular tool for gene editing in research and clinical contexts.
Nikolai A. Lomov, Vladimir S. Viushkov, Aleksei V. Zamalutdinov, Maria D. Sboeva, Mikhail A. Rubtsov   +4 more
doaj   +1 more source

Anti-CRISPR AcrIIA5 Potently Inhibits All Cas9 Homologs Used for Genome Editing

Cell Reports, 2019
Summary: CRISPR-Cas9 systems provide powerful tools for genome editing. However, optimal employment of this technology will require control of Cas9 activity so that the timing, tissue specificity, and accuracy of editing may be precisely modulated.
Bianca Garcia, Jooyoung Lee, Alireza Edraki, Yurima Hidalgo-Reyes, Steven Erwood, Aamir Mir, Chantel N. Trost, Uri Seroussi, Sabrina Y. Stanley, Ronald D. Cohn, Julie M. Claycomb, Erik J. Sontheimer, Karen L. Maxwell, Alan R. Davidson   +13 more
doaj   +1 more source

Molecular insights into DNA interference by CRISPR-associated nuclease-helicase Cas3 [PDF]

, 2014
Mobile genetic elements in bacteria are neutralized by a system based on clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins.
Bolt, Edward L., Gong, Bei, Jung, Che-Hun, Kim, Jeong-Sun, Shin, Minsang, Sun, Jiali, van der oost, John   +6 more
core   +3 more sources

Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungi [PDF]

, 2019
Background: Within the last years, numerous reports described successful application of the CRISPR nucleases Cas9 and Cpf1 for genome editing in filamentous fungi.
Haefner, Stefan, Kwon, Jin, Meyer, Vera, Schütze, Tabea, Spohner, Sebastian   +4 more
core   +1 more source

Artificial escape from XCI by DNA methylation editing of the CDKL5 gene. [PDF]

, 2020
A significant number of X-linked genes escape from X chromosome inactivation and are associated with a distinct epigenetic signature. One epigenetic modification that strongly correlates with X-escape is reduced DNA methylation in promoter regions. Here,
Anderson, Johnathon D, Buchanan, Fiona KB, Cameron, David, Carter, Jasmine L, Coggins, Nicole B, Deng, Peter, Fink, Kyle D, Gonzalez, Casiana E, Halmai, Julian ANM, Halmai, Julian ANM, Lock, Samantha R, Nolta, Jan, O'Geen, Henriette, Segal, David J, Waldo, Jennifer J   +14 more
core   +1 more source

Spontaneous Embedding of DNA Mismatches Within the RNA:DNA Hybrid of CRISPR-Cas9. [PDF]

, 2020
CRISPR-Cas9 is the forefront technology for editing the genome. In this system, the Cas9 protein is programmed with guide RNAs to process DNA sequences that match the guide RNA forming an RNA:DNA hybrid structure.
Hsu, Rohaine V, Medrano, Marco A, Mitchell, Brandon P, Narkhede, Yogesh B, Palermo, Giulia, Zewde, Nehemiah T   +5 more
core