Results 91 to 100 of about 34,514 (185)

TracrRNA reprogramming enables direct PAM-independent detection of RNA with diverse DNA-targeting Cas12 nucleases

Nature Communications
Many CRISPR-Cas immune systems generate guide (g)RNAs using trans-activating CRISPR RNAs (tracrRNAs). Recent work revealed that Cas9 tracrRNAs could be reprogrammed to convert any RNA-of-interest into a gRNA, linking the RNA’s presence to Cas9-mediated ...
Chunlei Jiao, Natalia L. Peeck, Jiaqi Yu, Mohammad Ghaem Maghami, Sarah Kono, Daphne Collias, Sandra L. Martinez Diaz, Rachael Larose, Chase L. Beisel   +8 more
doaj   +1 more source

CRISPR/Cas9 technology in tumor research and drug development application progress and future prospects

Frontiers in Pharmacology
The CRISPR/Cas9 system is an acquired immune defense mechanism that has evolved in bacteria and archaea to protect against viral and plasmid attacks. It consists of regularly spaced clusters of short palindromic repeats (CRISPR) and CRISPR-associated ...
Han Han, Xiaoyan Sun, Xiaoyun Guo, Jiaxin Wen, Xiaoming Zhao, Weiqiang Zhou   +5 more
doaj   +1 more source

TIGR-Tas and the Expanding Universe of RNA-Guided Genome Editing Systems: A New Era Beyond CRISPR-Cas

Genes
The recent discovery of TIGR-Tas (Tandem Interspaced Guide RNA–Targeting Systems) marks a major advance in the field of genome editing, introducing a new class of compact, programmable DNA-targeting systems that function independently of traditional CRISPR-Cas pathways.
openaire   +1 more source

Targeted gene modification in Fragaria vesca mediated by CRISPR/Cas9 system [PDF]

, 2016
Genome editing is becoming an important biotechnological tool for gene function analysis and crop improvement, being the CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeat-CRISPR associated protein 9) system the most widely used.
Martín-Pizarro, Carmen, Posé, David
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A Rapid Hairy Root-Based Platform for CRISPR/Cas Optimization and Guide RNA Validation in Lettuce

Plants
Cultivated lettuce (Lactuca sativa L.) is a major leafy crop and an emerging model for functional genomics within the Asteraceae family, supported by high-quality reference genomes and efficient transformation systems.
Alberico Di Pinto, Valentina Forte, Chiara D’Attilia, Marco Possenti, Barbara Felici, Floriana Augelletti, Giovanna Sessa, Monica Carabelli, Giorgio Morelli, Giovanna Frugis, Fabio D’Orso   +10 more
doaj   +1 more source

Compatibility of Breeding Techniques in Organic Systems [PDF]

, 2017
Introduction The rapid development of genetic engineering techniques is leading to a level of genetic disruption never experienced before. In order to safeguard organic integrity and to ensure organic food will continue to meet the highest consumer ...

core  

Testing multiplexed anti-ASFV CRISPR-Cas9 in reducing African swine fever virus

Microbiology Spectrum
African swine fever (ASF) is a highly fatal viral disease that poses a significant threat to domestic pigs and wild boars globally. In our study, we aimed to explore the potential of a multiplexed CRISPR-Cas system in suppressing ASFV replication and ...
Zezhong Zheng, Lei Xu, Yangbin Gao, Hongwei Dou, Yixuan Zhou, Xu Feng, Xiangjun He, Zhen Tian, Lingling Song, Guolong Mo, Jiapan Hu, Hongye Zhao, Hongjiang Wei, George M. Church, Luhan Yang   +14 more
doaj   +1 more source

Mapping genetic interactions in cancer: a road to rational combination therapies. [PDF]

, 2019
The discovery of synthetic lethal interactions between poly (ADP-ribose) polymerase (PARP) inhibitors and BRCA genes, which are involved in homologous recombination, led to the approval of PARP inhibition as a monotherapy for patients with BRCA1/2 ...
Krogan, Nevan J, Tutuncuoglu, Beril
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Robust and stable transcriptional repression in Giardia using CRISPRi. [PDF]

, 2019
Giardia lamblia is a binucleate protistan parasite causing significant diarrheal disease worldwide. An inability to target Cas9 to both nuclei, combined with the lack of nonhomologous end joining and markers for positive selection, has stalled the ...
Booker, J, Dawson, Scott C, Hagen, KD, Jones, K, McInally, SG, Nguyen, K, Nosala, C, Williams, J   +7 more
core   +1 more source

Unveiling the invisible genomic dynamics

Experimental and Molecular Medicine
CRISPR-based imaging technologies have emerged as powerful tools for visualizing specific genomic loci, providing groundbreaking insights into chromatin structure and dynamics. Here, in this Review, we discuss the development and recent advances in these
Jiwoong Kwon, Yeji Park, Taekjip Ha
doaj   +1 more source