Results 1 to 10 of about 331,418 (212)
RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences
Biomolecules, 2015 The RNA cleavage activity of RNase P can be employed to decrease the levels of specific RNAs and to study their function or even to eradicate pathogens. Two different technologies have been developed to use RNase P as a tool for RNA knockdown.
Merel Derksen +2 more
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Short RNA guides cleavage by eukaryotic RNase III.
PLoS ONE, 2007 In eukaryotes, short RNAs guide a variety of enzymatic activities that range from RNA editing to translation repression. It is hypothesized that pre-existing proteins evolved to bind and use guide RNA during evolution.
Bruno Lamontagne, Sherif Abou Elela
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A Simplified Guide RNA Synthesis Protocol for SNAP- and Halo-Tag-Based RNA Editing Tools [PDF]
MoleculesSNAP-tag and Halo-tag have been employed to achieve targeted RNA editing by directing the deaminase domain of human ADAR to specific sites in the transcriptome.
Daniel Tobias Hofacker +4 more
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Cell Reports, 2018 Summary: Prokaryotic Argonaute (Ago) proteins were recently shown to target foreign genetic elements, thus making them a perfect model for studies of interference mechanisms.
Yiwei Liu +6 more
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CRISPy-web 3.0: A unified platform for multi-modal guide RNA design for CRISPR and TnpB genome editing applications [PDF]
Synthetic and Systems BiotechnologyCRISPy-web is an interactive web-based platform designed for rational guide RNA design in CRISPR-Cas9-based genome editing systems. Here, we present CRISPy-web 3.0 (https://crispy.secondarymetabolites.org/), an upgraded and versatile version that extends
Sihan Yang +6 more
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Multi-locus CRISPRi targeting with a single truncated guide RNA [PDF]
Nature CommunicationsA critical goal in functional genomics is evaluating which non-coding elements contribute to gene expression, cellular function, and disease. Functional characterization remains a challenge due to the abundance and complexity of candidate elements. Here,
Molly M. Moore +14 more
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Mitochondrial RNA editing in Trypanoplasma borreli: New tools, new revelations
Computational and Structural Biotechnology Journal, 2022 The kinetoplastids are unicellular flagellates that derive their name from the ‘kinetoplast’, a region within their single mitochondrion harboring its organellar genome of high DNA content, called kinetoplast (k) DNA.
Evgeny S. Gerasimov +8 more
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RNA Biology, 2023 Gene editing using CRISPR/Cas (clustered regularly interspaced palindromic repeats/CRISPR-associated) is under development as a therapeutic tool for the modification of genes in eukaryotic cells.
David A. Armstrong +5 more
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Dynamic Genome Editing Using In Vivo Synthesized Donor ssDNA in Escherichia coli
Cells, 2020 As a key element of genome editing, donor DNA introduces the desired exogenous sequence while working with other crucial machinery such as CRISPR-Cas or recombinases.
Min Hao +5 more
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Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens
Cell Genomics, 2021 Summary: The recent characterization of RNA-targeting CRISPR nucleases has enabled diverse transcriptome engineering and screening applications that depend crucially on prediction and selection of optimized CRISPR guide RNAs (gRNAs).
Xinyi Guo +6 more
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