Results 11 to 20 of about 323,472 (305)
Structure of yeast Argonaute with guide RNA [PDF]
Nature, 2012 The RNA-induced silencing complex, comprising Argonaute and guide RNA, mediates RNA interference. Here we report the 3.2 Å crystal structure of Kluyveromyces polysporus Argonaute (KpAGO) fortuitously complexed with guide RNA originating from small-RNA duplexes autonomously loaded by recombinant KpAGO.
Kotaro Nakanishi +2 more
exaly +6 more sources
RNA Interference by Single- and Double-stranded siRNA With a DNA Extension Containing a 3′ Nuclease-resistant Mini-hairpin Structure [PDF]
Molecular Therapy: Nucleic Acids, 2014 Selective gene silencing by RNA interference (RNAi) involves double-stranded small interfering RNA (ds siRNA) composed of single-stranded (ss) guide and passenger RNAs.
Simon J Allison, Jo Milner
doaj +2 more sources
Nucleic Acids Research, 1998 Guide RNAs (gRNAs) are small, metabolically stable RNA molecules which perform a pivotal, template-like function during the RNA editing process in kinetoplastid protozoa. The gRNA database currently contains 250 guide RNA sequences as well as secondary and tertiary structure models and other relevant information.
Augustine E. Souza, H. Ulrich Göringer
openaire +3 more sources
Nucleic Acids Research, 1997 The RNA editing process in protozoan parasites is controlled by small RNA molecules known as guide RNAs (gRNAs). The gRNA database is a comprehensive compilation of published guide RNA sequences from eight different kinetoplastid organisms. In addition to the RNA primary sequences, information on the gene localization, the experimental verification of ...
Augustine E. Souza +2 more
openaire +3 more sources
RNase P-Mediated Sequence-Specific Cleavage of RNA by Engineered External Guide Sequences
Biomolecules, 2015 The RNA cleavage activity of RNase P can be employed to decrease the levels of specific RNAs and to study their function or even to eradicate pathogens. Two different technologies have been developed to use RNase P as a tool for RNA knockdown.
Merel Derksen +2 more
doaj +3 more sources
The guide RNA database (3.0) [PDF]
Nucleic Acids Research, 1999 The RNA editing process within the mitochondria of kinetoplastid organisms is controlled by small, trans -acting RNA molecules referred to as guide RNAs. The guide RNA database is a compilation of published guide RNA sequences, currently containing 254 entries from 11 different organisms.
Sonja Hinz, H. Ulrich Göringer
openaire +3 more sources
A Simplified Guide RNA Synthesis Protocol for SNAP- and Halo-Tag-Based RNA Editing Tools [PDF]
MoleculesSNAP-tag and Halo-tag have been employed to achieve targeted RNA editing by directing the deaminase domain of human ADAR to specific sites in the transcriptome.
Daniel Tobias Hofacker +4 more
doaj +2 more sources
Cell Reports, 2018 Summary: Prokaryotic Argonaute (Ago) proteins were recently shown to target foreign genetic elements, thus making them a perfect model for studies of interference mechanisms.
Yiwei Liu +6 more
doaj +3 more sources
CRISPy-web 3.0: A unified platform for multi-modal guide RNA design for CRISPR and TnpB genome editing applications [PDF]
Synthetic and Systems BiotechnologyCRISPy-web is an interactive web-based platform designed for rational guide RNA design in CRISPR-Cas9-based genome editing systems. Here, we present CRISPy-web 3.0 (https://crispy.secondarymetabolites.org/), an upgraded and versatile version that extends
Sihan Yang +6 more
doaj +2 more sources
Multi-locus CRISPRi targeting with a single truncated guide RNA [PDF]
Nature CommunicationsA critical goal in functional genomics is evaluating which non-coding elements contribute to gene expression, cellular function, and disease. Functional characterization remains a challenge due to the abundance and complexity of candidate elements. Here,
Molly M. Moore +14 more
doaj +2 more sources