Results 21 to 30 of about 323,472 (305)

Mitochondrial RNA editing in Trypanoplasma borreli: New tools, new revelations

Computational and Structural Biotechnology Journal, 2022
The kinetoplastids are unicellular flagellates that derive their name from the ‘kinetoplast’, a region within their single mitochondrion harboring its organellar genome of high DNA content, called kinetoplast (k) DNA.
Evgeny S. Gerasimov, Dmitry A. Afonin, Oksana A. Korzhavina, Julius Lukeš, Ross Low, Neil Hall, Kevin Tyler, Vyacheslav Yurchenko, Sara L. Zimmer   +8 more
doaj   +1 more source

PlmCas12e (CasX2) cleavage of CCR5: impact of guide RNA spacer length and PAM sequence on cleavage activity

RNA Biology, 2023
Gene editing using CRISPR/Cas (clustered regularly interspaced palindromic repeats/CRISPR-associated) is under development as a therapeutic tool for the modification of genes in eukaryotic cells.
David A. Armstrong, Taylor R. Hudson, Christine A. Hodge, Thomas H. Hampton, Alexandra L. Howell, Matthew S. Hayden   +5 more
doaj   +1 more source

Dynamic Genome Editing Using In Vivo Synthesized Donor ssDNA in Escherichia coli

Cells, 2020
As a key element of genome editing, donor DNA introduces the desired exogenous sequence while working with other crucial machinery such as CRISPR-Cas or recombinases.
Min Hao, Zhaoguan Wang, Hongyan Qiao, Peng Yin, Jianjun Qiao, Hao Qi   +5 more
doaj   +1 more source

Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens

Cell Genomics, 2021
Summary: The recent characterization of RNA-targeting CRISPR nucleases has enabled diverse transcriptome engineering and screening applications that depend crucially on prediction and selection of optimized CRISPR guide RNAs (gRNAs).
Xinyi Guo, Jahan A. Rahman, Hans-Hermann Wessels, Alejandro Méndez-Mancilla, Daniel Haro, Xinru Chen, Neville E. Sanjana   +6 more
doaj   +1 more source

Crosstalk between CRISPR-Cas9 and the human transcriptome

Nature Communications, 2022
The off-target effects of CRISPR-Cas9 are thought to be mediated by its cognate guide RNA. Here the authors show that Cas9 independently interacts with the human transcriptome, correlating with elevated RNA editing even under guide RNA co-expression.
Aaron A. Smargon, Assael A. Madrigal, Brian A. Yee, Kevin D. Dong, Jasmine R. Mueller, Gene W. Yeo   +5 more
doaj   +1 more source

Characterization of a Programmable Argonaute Nuclease from the Mesophilic Bacterium Rummeliibacillus suwonensis

Biomolecules, 2022
Prokaryotic Argonautes (pAgos) from mesophilic bacteria are attracting increasing attention for their genome editing potential. So far, it has been reported that KmAgo from Kurthia massiliensis can utilize DNA and RNA guide of any sequence to effectively
Xiaoman Jiang, Yang Liu, Qi Liu, Lixin Ma   +3 more
doaj   +1 more source

Proximity-activated guide RNA of CRISPR–Cas12a for programmable diagnostic detection and gene regulation [PDF]

Nucleic Acids Research
Zhian Hu, Shen Ling, Sichun Zhang
exaly   +2 more sources

A Guide to Trajectory Inference and RNA Velocity [PDF]

, 2021
Abstract Technological developments have led to an explosion of high-throughput single cell data, which are revealing unprecedented perspectives on cell identity. Recently, significant attention has focused on investigating, from single-cell RNA-sequencing (scRNA-seq) data, cellular dynamic processes, such as cell differentiation, cell ...
Weiler, Philipp, Van den Berge, Koen, Street, Kelly, Tiberi, Simone   +3 more
openaire   +4 more sources

Structural basis for sequence-specific recognition of guide and target strands by the Archaeoglobus fulgidus Argonaute protein

Scientific Reports, 2023
Argonaute (Ago) proteins are found in all three domains of life. The best-characterized group is eukaryotic Argonautes (eAgos). Being the structural core of RNA interference machinery, they use guide RNA molecules for RNA targeting.
Elena Manakova, Edvardas Golovinas, Reda Pocevičiūtė, Giedrius Sasnauskas, Algirdas Grybauskas, Saulius Gražulis, Mindaugas Zaremba   +6 more
doaj   +1 more source

MAIGRET: a CRISPR-based immunoassay that employs antibody-induced cell-free transcription of CRISPR guide RNA strands [PDF]

Nucleic Acids Research
Francesca Miceli, Daniela Sorrentino, Alessandro Porchetta   +2 more
exaly   +2 more sources