Results 41 to 50 of about 2,639,376 (307)
RNA processing: Pocket guides to ribosomal RNA [PDF]
Current Biology, 1997 The functional role of a recently identified class of small nucleolar (sno) RNAs has been elucidated: the 'box H/ACA' snoRNAs act as guide RNAs, specifying the position of evolutionarily conserved pseudouridines in ribosomal (r)RNA via an rRNA-snoRNA base-pairing interaction that forms a 'pseudouridine pocket'.
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MicroRNA-target binding structures mimic microRNA duplex structures in humans. [PDF]
PLoS ONE, 2014 Traditionally, researchers match a microRNA guide strand to mRNA sequences using sequence comparisons to predict its potential target genes. However, many of the predictions can be false positives due to limitations in sequence comparison alone.
Xi Chen, Lu Shen, Hui-Hsien Chou
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A guide to ions and RNA structure [PDF]
RNA, 2004 RNA folding into stable tertiary structures is remarkably sensitive to the concentrations and types of cations present; an understanding of the physical basis of ion-RNA interactions is therefore a prerequisite for a quantitative accounting of RNA stability.
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A hepatitis C virus cis-acting replication element forms a long-range RNA-RNA interaction with upstream RNA sequences in NS5B [PDF]
, 2008 The genome of hepatitis C virus (HCV) contains cis-acting replication elements (CREs) comprised of RNA stem-loop structures located in both the 5' and 3' non-coding regions (NCR), and in the NS5B coding sequence.
Diviney, Sinéad +22 more
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, 2021 Robust methods are critical for testing the in vivo regulatory mechanism of RNA binding proteins. Here we report improvement of a protein–mRNA tethering assay to probe the function of an RNA binding protein in its natural context within the C.
Kimble, Judith +3 more
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Mechanistic Insights into Archaeal and Human Argonaute Substrate Binding and Cleavage Properties.
PLoS ONE, 2016 Argonaute (Ago) proteins from all three domains of life are key players in processes that specifically regulate cellular nucleic acid levels.
Sarah Willkomm +3 more
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RNA-programmed genome editing in human cells
eLife, 2013 Type II CRISPR immune systems in bacteria use a dual RNA-guided DNA endonuclease, Cas9, to cleave foreign DNA at specific sites. We show here that Cas9 assembles with hybrid guide RNAs in human cells and can induce the formation of double-strand DNA ...
Martin Jinek +5 more
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RNA-guided RNA silencing by an Asgard archaeal Argonaute
Nature Communications, 2023 Abstract Argonaute proteins are the central effectors of RNA-guided RNA silencing pathways in eukaryotes, playing crucial roles in gene repression and defense against viruses and transposons. Eukaryotic Argonautes are subdivided into two clades: AGOs generally facilitate miRNA- or siRNA-mediated silencing, while ...
Carolien Bastiaanssen +13 more
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Diverging affinity of tospovirus RNA silencing suppressor proteins, NSs, for various RNA duplex molecules [PDF]
, 2010 The tospovirus NSs protein was previously shown to suppress the antiviral RNA silencing mechanism in plants. Here the biochemical analysis of NSs proteins from different tospoviruses, using purified NSs or NSs containing cell extracts, is described.
Kormelink, R.J.M. +16 more
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Craspase is a CRISPR RNA-guided, RNA-activated protease
Science, 2022 The CRISPR-Cas type III-E RNA-targeting effector complex gRAMP/Cas7-11 is associated with a caspase-like protein (TPR-CHAT/Csx29) to form Craspase (CRISPR-guided caspase). Here, we use cryo–electron microscopy snapshots of Craspase to explain its target RNA cleavage and protease activation mechanisms.
Hu, Chunyi +11 more
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