Results 41 to 50 of about 323,472 (305)
RNA-guided RNA silencing by an Asgard archaeal Argonaute
Nature Communications, 2023 Abstract Argonaute proteins are the central effectors of RNA-guided RNA silencing pathways in eukaryotes, playing crucial roles in gene repression and defense against viruses and transposons. Eukaryotic Argonautes are subdivided into two clades: AGOs generally facilitate miRNA- or siRNA-mediated silencing, while ...
Carolien Bastiaanssen +13 more
openaire +3 more sources
Editosome Accessory Factors KREPB9 and KREPB10 in Trypanosoma brucei [PDF]
, 2012 Multiprotein complexes, called editosomes, catalyze the uridine insertion and deletion RNA editing that forms translatable mitochondrial mRNAs in kinetoplastid parasites.
Acestor, Nathalie +5 more
core +1 more source
Structural transitions upon guide RNA binding and their importance in Cas12g-mediated RNA cleavage
PLoS Genetics, 2023 Cas12g is an endonuclease belonging to the type V RNA-guided CRISPR-Cas family. It is known for its ability to cleave RNA substrates using a conserved endonuclease active site located in the RuvC domain. In this study, we determined the crystal structure
Mengxi Liu +8 more
doaj +2 more sources
Domain orientation in the RNA helicase YxiN and the role of conformational changes for RNA unwinding [PDF]
, 2010 The RNA helicase YxiN from Bacillus subtilis is a member of the family of DEAD box proteins. YxiN is able to unwind RNA double strands in an ATP-dependent manner. The ability to catalyse RNA rearrangement is in vivo presumably necessary for the bacterial
Karow, Anne R.
core +1 more source
RNA processing: Pocket guides to ribosomal RNA [PDF]
Current Biology, 1997 The functional role of a recently identified class of small nucleolar (sno) RNAs has been elucidated: the 'box H/ACA' snoRNAs act as guide RNAs, specifying the position of evolutionarily conserved pseudouridines in ribosomal (r)RNA via an rRNA-snoRNA base-pairing interaction that forms a 'pseudouridine pocket'.
openaire +2 more sources
Craspase is a CRISPR RNA-guided, RNA-activated protease
Science, 2022 The CRISPR-Cas type III-E RNA-targeting effector complex gRAMP/Cas7-11 is associated with a caspase-like protein (TPR-CHAT/Csx29) to form Craspase (CRISPR-guided caspase). Here, we use cryo–electron microscopy snapshots of Craspase to explain its target RNA cleavage and protease activation mechanisms.
Hu, Chunyi +11 more
openaire +4 more sources
Engineering CRISPR guide RNAs for programmable RNA sensors
Biochemical Society Transactions, 2023 As the most valuable feature of the CRISPR system, the programmability based on Watson–Crick base pairing has been widely exploited in engineering RNA sensors. The base pairing in these systems offers a connection between the RNA of interest and the CRISPR effector, providing a highly specific mechanism for RNA detection both in vivo and in vitro.
Yang Liu, Wei Liu, Baojun Wang
openaire +3 more sources
Inhibition of HIV-1 replication using the CRISPR/cas9-no NLS system as a prophylactic strategy
Heliyon, 2022 Globally, it is estimated that 43 million people are living with human immunodeficiency virus type 1 (HIV-1), and there are more than 600,000 acquired immunodeficiency syndrome (AIDS)-related deaths in 2020.
Ali Salimi-Jeda +5 more
doaj +1 more source
A guide to ions and RNA structure [PDF]
RNA, 2004 RNA folding into stable tertiary structures is remarkably sensitive to the concentrations and types of cations present; an understanding of the physical basis of ion-RNA interactions is therefore a prerequisite for a quantitative accounting of RNA stability.
openaire +2 more sources
MicroRNA-target binding structures mimic microRNA duplex structures in humans. [PDF]
PLoS ONE, 2014 Traditionally, researchers match a microRNA guide strand to mRNA sequences using sequence comparisons to predict its potential target genes. However, many of the predictions can be false positives due to limitations in sequence comparison alone.
Xi Chen, Lu Shen, Hui-Hsien Chou
doaj +1 more source