Results 61 to 70 of about 323,472 (305)

CASowary: CRISPR-Cas13 guide RNA predictor for transcript depletion

BMC Genomics, 2022
Background Recent discovery of the gene editing system - CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) associated proteins (Cas), has resulted in its widespread use for improved understanding of a variety of biological systems ...
Alexander Krohannon, Mansi Srivastava, Simone Rauch, Rajneesh Srivastava, Bryan C. Dickinson, Sarath Chandra Janga   +5 more
doaj   +1 more source

The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a [PDF]

Cell, 2017
Cas13a, a type VI-A CRISPR-Cas RNA-guided RNA ribonuclease, degrades invasive RNAs targeted by CRISPR RNA (crRNA) and has potential applications in RNA technology. To understand how Cas13a is activated to cleave RNA, we have determined the crystal structure of Leptotrichia buccalis (Lbu) Cas13a bound to crRNA and its target RNA, as well as the cryo-EM ...
Liang, Liu, Xueyan, Li, Jun, Ma, Zongqiang, Li, Lilan, You, Jiuyu, Wang, Min, Wang, Xinzheng, Zhang, Yanli, Wang   +8 more
openaire   +2 more sources

Improved annotation of 3' untranslated regions and complex loci by combination of strand-specific direct RNA sequencing, RNA-seq and ESTs [PDF]

, 2014
The reference annotations made for a genome sequence provide the framework for all subsequent analyses of the genome. Correct and complete annotation in addition to the underlying genomic sequence is particularly important when interpreting the results ...
Junfang Song, Geoffrey J Barton, Schurch Nicholas J., Brown, Sara J., Gordon G Simpson, Nicholas J Schurch, McLean, W. H. Irwin, Song Junfang, Simpson Gordon G., Sherstnev, Alexander, Brown Sara J., Alexander Sherstnev, Song, Junfang, McLean W. H. Irwin, Duc, Celine, Kate G Storey, Storey, Kate G., Schurch, Nicholas J., Cole Christian, Sherstnev Alexander, Simpson, Gordon G., Céline Duc, W H Irwin McLean, Cole, Christian; id_orcid, Christian Cole, Barton Geoffrey J., Sara J Brown, Duc Céline, Barton, Geoffrey J.; id_orcid, Storey Kate G.   +29 more
core   +1 more source

sgRNA amount is a limiting factor in adenine base editing using RNA LNPs

Molecular Therapy: Nucleic Acids
Base editors have emerged as powerful tools for precise genome editing, offering significant therapeutic potential. A critical challenge lies in optimizing the delivery and dosage of single-guide RNA to maximize on-target editing efficiency while ...
Alexandra Birkenshaw, Tyler Thomson, Mai P. Truong, Yui Komaki, Nadine Ramsden, Ana Timpano, Cassie Huang, Anna K. Blakney, Daniel Z. Kurek, Jayesh Kulkarni, Colin J.D. Ross   +10 more
doaj   +1 more source

RNA editing: Guided but not templated?

Cell, 1990
Article synthese etabli a partir de quatre publications recentes sur l'«edition RNA», son origine, son mode d ...
A M, Weiner, N, Maizels
openaire   +2 more sources

Polyvalent guide RNAs for CRISPR antivirals

iScience, 2022
CRISPR effector Cas13 recognizes and degrades RNA molecules that are complementary to its guide RNA (gRNA) and possesses potential as an antiviral biotechnology because it can degrade viral mRNA and RNA genomes. Because multiplexed targeting is a critical strategy to improve viral suppression, we developed a strategy to design of gRNAs where individual
Rammyani Bagchi, Rachel Tinker-Kulberg, Mohammad Salehin, Tinku Supakar, Sydney Chamberlain, Ayalew Ligaba-Osena, Eric A. Josephs   +6 more
openaire   +3 more sources

Claudin‐6 Protein Expression in Atypical Teratoid/Rhabdoid Tumors Is Strongly Enriched in the Molecular Subgroup AT/RT‐TYR

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Claudin‐6 has emerged as a promising immunotherapeutic target, yet protein‐level data in atypical teratoid/rhabdoid tumors (AT/RTs) have been inconsistent. We analyzed 36 well‐characterized AT/RT samples and found membranous claudin‐6 protein expression in 58% of cases, with striking enrichment in the molecular subgroup AT/RT‐TYR (100%) and ...
Victoria E. Fincke, Christian Thomas, Michael C. Frühwald, Pascal D. Johann, Martin Hasselblatt   +4 more
wiley   +1 more source

Complete probabilistic analysis of RNA shapes

, 2006
Voß B, Giegerich R, Rehmsmeier M. Complete probabilistic analysis of RNA shapes. BMC Biology. 2006;4(1): 5.Background: Soon after the first algorithms for RNA folding became available, it was recognised that the prediction of only one energetically ...
Marc Rehmsmeier, Voß, Björn, Rehmsmeier, Marc, Björn Voß, Rehmsmeier Marc, Robert Giegerich, Giegerich, Robert, Giegerich Robert, Voß Björn   +8 more
core   +1 more source

Viral RNA Interactome: The Ultimate Researcher’s Guide to RNA–Protein Interactions

Viruses
RNA molecules in the cell are bound by a multitude of RNA-binding proteins (RBPs) with a variety of regulatory consequences. Often, interactions with these RNA-binding proteins are facilitated by the complex secondary and tertiary structures of RNA ...
Wesley A. Hanson, Gabriel A. Romero Agosto, Silvi Rouskin   +2 more
doaj   +1 more source

RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex [PDF]

Cell, 2009
Compelling evidence indicates that the CRISPR-Cas system protects prokaryotes from viruses and other potential genome invaders. This adaptive prokaryotic immune system arises from the clustered regularly interspaced short palindromic repeats (CRISPRs) found in prokaryotic genomes, which harbor short invader-derived sequences, and the CRISPR-associated (
Hale, Caryn R., Zhao, Peng, Olson, Sara, Duff, Michael O., Graveley, Brenton R., Wells, Lance, Terns, Rebecca M., Terns, Michael P.   +7 more
openaire   +2 more sources