Results 51 to 60 of about 323,472 (305)

Cas9 deactivation with photocleavable guide RNAs [PDF]

Molecular Cell, 2021
Precise control of CRISPR-Cas9 would improve its safety and applicability. Controlled CRISPR inhibition is a promising approach but is complicated by separate inhibitor delivery, incomplete deactivation, and slow kinetics. To overcome these obstacles, we engineered photocleavable guide RNAs (pcRNAs) that endow Cas9 nucleases and base editors with a ...
Roger S, Zou, Yang, Liu, Bin, Wu, Taekjip, Ha   +3 more
openaire   +2 more sources

Computational discovery of animal small RNA genes and targets [PDF]

, 2007
Though recently discovered, small RNAs appear to play a wealth of regulatory roles, being involved in degradation of target mRNAs, translation silencing of target genes, chromatin remodeling and transposon silencing.
Gaidatzis, Dimosthenis
core   +1 more source

RNA-guided gene editing [PDF]

Nature Methods, 2013
Targeted gene modification can be guided by programmable RNA in bacteria, zebrafish and mammalian cells.
openaire   +2 more sources

Biological roles of DExH RNA helicase, RHAU [PDF]

, 2007
In this thesis, I have described the work carried out on a single protein called RHAU, dealing with aspects of protein localization in cells, the regulation of global gene expression by different mechanisms, and cellular stress-responses.
Iwamoto, Fumiko
core   +1 more source

Mechanistic Insights into Archaeal and Human Argonaute Substrate Binding and Cleavage Properties.

PLoS ONE, 2016
Argonaute (Ago) proteins from all three domains of life are key players in processes that specifically regulate cellular nucleic acid levels.
Sarah Willkomm, Adrian Zander, Dina Grohmann, Tobias Restle   +3 more
doaj   +1 more source

RNA-programmed genome editing in human cells

eLife, 2013
Type II CRISPR immune systems in bacteria use a dual RNA-guided DNA endonuclease, Cas9, to cleave foreign DNA at specific sites. We show here that Cas9 assembles with hybrid guide RNAs in human cells and can induce the formation of double-strand DNA ...
Martin Jinek, Alexandra East, Aaron Cheng, Steven Lin, Enbo Ma, Jennifer Doudna   +5 more
doaj   +1 more source

Protocol: high throughput silica-based purification of RNA from Arabidopsis seedlings in a 96-well format [PDF]

, 2011
The increasing popularity of systems-based approaches to plant research has resulted in a demand for high throughput (HTP) methods to be developed. RNA extraction from multiple samples in an experiment is a significant bottleneck in performing systems ...
Kane Steven, Kerr Lorraine E, Lorraine E Kerr, Salvo-Chirnside, Eliane, Kerr, Lorraine E, Steven Kane, Salvo-Chirnside Eliane, Kerr, Lorraine E., Kane, Steven, Eliane Salvo-Chirnside   +9 more
core   +1 more source

Short RNA guides cleavage by eukaryotic RNase III.

PLoS ONE, 2007
In eukaryotes, short RNAs guide a variety of enzymatic activities that range from RNA editing to translation repression. It is hypothesized that pre-existing proteins evolved to bind and use guide RNA during evolution.
Bruno Lamontagne, Sherif Abou Elela
doaj   +1 more source

Aptamer- and Ribozyme-Engineered sgRNAs for Conditional Control of CRISPR/Cas9 Function

Frontiers in Bioscience-Landmark
The clustered regularly interspaced short palindromic repeats CRISPR-associated protein 9 (CRISPR/Cas9) system has emerged as a versatile platform for genome editing, transcriptional regulation, and chromosomal imaging.
Wei Wang, Liting Li, Xiaohui Li, Qiulong Zhang, Yan Liu   +4 more
doaj   +1 more source

Structural basis for CRISPR RNA-guided DNA recognition by Cascade [PDF]

, 2011
The CRISPR (clustered regularly interspaced short palindromic repeats) immune system in prokaryotes uses small guide RNAs to neutralize invading viruses and plasmids.
Beijer, Marieke R., Mark J Dickman, Dickman, Mark J.,, Snijders, AP, Matthijs M Jore, Rolf Wagner, Sakharam P Waghmare, Beijer, Marieke, Wagner, Rolf, Brouns, Stan J. J., Barendregt, Arjan,, Snijders, Ambrosius, Dickman, Mark, Oost, J., van der, Arjan Barendregt, Boekema, Egbert, Dickman, Mark J., Bultema, J.B., Boekema, EJ, Waghmare, Sakharam P.,, Brouns, S.J.J., Wurm, Reinhild,, Westra, ER, Pul, U, Boekema, Egbert J., Doudna, Jennifer, Pul, Ümit,, Beijer, Marieke R.,, Egbert J Boekema, Jelle B Bultema, Westra, Edze, Snijders, Ambrosius P.L.,, Lundgren, Magnus, Jennifer A Doudna, Bultema, Jelle B.,, Wagner, R, John van der Oost, Westra, Edze R.,, van der Oost, J, Beijer, M.R., Wurm, R, Beijer, MR, Edze R Westra, Doudna, Jennifer A.,, Kaihong Zhou, Duijn, E., van, Jore, M.M., Heck, AJ, van der Oost, J., Waghmare, Sakharam, Jore, MM, Westra, E.R., Brouns, SJ, Doudna, Jennifer A., Wurm, Reinhild, Wagner, Rolf,, Ambrosius P L Snijders, Zhou, K, Barendregt, Arjan, Albert J R Heck, Brouns, Stan J.J.,, Magnus Lundgren, Pul, Uemit, Boekema, Egbert J.,, van Duijn, Esther, Snijders, Ambrosius P. L., Heck, Albert J. R., Lundgren, M, Pul, Ümit, Zhou, Kaihong,, Oost, John van der,, Blake Wiedenheft, Wiedenheft, Blake,, Wiedenheft, Blake, Waghmare, SP, van der Oost, John, Reinhild Wurm, Stan J J Brouns, Brouns, Stan, Jore, Matthijs M.,, Waghmare, Sakharam P., Bultema, Jelle, Zhou, Kaihong, Dickman, MJ, Lundgren, Magnus,, Barendregt, A, Bultema, JB, Jore, Matthijs, Duijn, Esther van,, Jore, Matthijs M., Lundgren, N.M.J., Ümit Pul, Marieke R Beijer, Wiedenheft, B, van Duijn, E., Heck, Albert J.R.,, Heck, Albert, Bultema, Jelle B., van Duijn, E, Westra, Edze R., Esther van Duijn, Doudna, JA   +101 more
core   +1 more source