Results 51 to 60 of about 2,639,376 (307)
sgRNA amount is a limiting factor in adenine base editing using RNA LNPs
Molecular Therapy: Nucleic AcidsBase editors have emerged as powerful tools for precise genome editing, offering significant therapeutic potential. A critical challenge lies in optimizing the delivery and dosage of single-guide RNA to maximize on-target editing efficiency while ...
Alexandra Birkenshaw +10 more
doaj +1 more source
Pediatric Blood &Cancer, EarlyView.ABSTRACT As part of the European Cooperative Study Group for Paediatric Rare Tumours initiative, we developed standard clinical practice guidelines for ovarian sex cord stromal tumors, based on comprehensive national and international cohort analyses, literature review, and a final expert consensus conference.
Dominik T. Schneider +15 more
wiley +1 more source
Computational discovery of animal small RNA genes and targets [PDF]
, 2007 Though recently discovered, small RNAs appear to play a wealth of regulatory roles, being involved in degradation of target mRNAs, translation silencing of target genes, chromatin remodeling and transposon silencing.
Gaidatzis, Dimosthenis
core +1 more source
Viral RNA Interactome: The Ultimate Researcher’s Guide to RNA–Protein Interactions
VirusesRNA molecules in the cell are bound by a multitude of RNA-binding proteins (RBPs) with a variety of regulatory consequences. Often, interactions with these RNA-binding proteins are facilitated by the complex secondary and tertiary structures of RNA ...
Wesley A. Hanson +2 more
doaj +1 more source
FEBS Letters, EarlyView.Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi +5 more
wiley +1 more source
Updated guide to RNA quantification by RNA sequencing and reverse transcription-qPCR
Molecules and Cells: RNA sequencing (RNA-seq) and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) are widely used for RNA quantification. RNA species with distinct structural and biogenetic features require specific computational and experimental ...
Dajeong Bong +3 more
doaj +1 more source
Diversity and complexity in neural organoids
FEBS Letters, EarlyView.Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley +1 more source
Turnip Mosaic Virus‐Based gRNA Delivery System for Plant Genome Editing
Plant DirectPlant virus‐based gRNA delivery systems offer a rapid alternative to stable transformation for CRISPR‐mediated genome editing, but potyvirus‐based platforms in Cas9‐expressing plants are still underexplored. Here, we developed a turnip mosaic virus (TuMV)
Ekkachai Khwanbua +3 more
doaj +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
Nature Methods, 2013 Targeted gene modification can be guided by programmable RNA in bacteria, zebrafish and mammalian cells.
openaire +2 more sources