Results 31 to 40 of about 34,514 (185)

Deep learning in CRISPR-Cas systems: a review of recent studies

Frontiers in Bioengineering and Biotechnology, 2023
In genetic engineering, the revolutionary CRISPR-Cas system has proven to be a vital tool for precise genome editing. Simultaneously, the emergence and rapid evolution of deep learning methodologies has provided an impetus to the scientific exploration ...
Minhyeok Lee
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Modulating signaling networks by CRISPR/Cas9-mediated transposable element insertion [PDF]

, 2018
In a recent past, transposable elements (TEs) were referred to as selfish genetic components only capable of copying themselves with the aim of increasing the odds of being inherited.
A Bolotin, A Burt, A Hammond, AP Lorenzetti, B McClintock, BA Castilho, C Feschotte, C Feschotte, C Feschotte, C Kettlun, C Lu, C Ye, CN Hancock, DBT Cox, DJ Garfinkel, F Palazzoli, FA Ran, FJM Mojica, G Feng, G Mai, G Yang, H Kuang, H Mao, HT Dong, IK Jordan, J Hou, J Li, J Paszkowski, JA Doudna, JB Owens, JD Hollister, JE DiCarlo, JI Qüesta, JK Baillie, JM Casacuberta, K Kikuchi, K Naito, K Naito, K Shirasawa, KD Kim, KM Esvelt, KS Makarova, L Bortesi, L Cong, LC Li, LM Vaschetto, Luis María Vaschetto, M Cowley, M Jinek, M Momose, M Patel, M Szabo, M Turner, N Buchon, N Jiang, N Jiang, N Jiang, Q Zhang, R Barrangou, R Jansen, RM Walsh, SD Molyneux, SR Wessler, SR Yant, T Mourier, T Sakuma, T Singer, VM Gantz, W Matsunaga, WC Lai, X Li, X Li, X Lin, X Shan, Y Ishino, Y Yan, ZN Adelman   +76 more
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In silico Analysis Suggests Common Appearance of scaRNAs in Type II Systems and Their Association With Bacterial Virulence

Frontiers in Genetics, 2018
In addition to its well-established defense function, CRISPR/Cas can also exhibit crucial non-canonical activity through endogenous gene expression regulation, which was found to mainly affect bacterial virulence.
Jelena Guzina, Jelena Guzina, Wei-Hua Chen, Tamara Stankovic, Magdalena Djordjevic, Evgeny Zdobnov, Marko Djordjevic   +6 more
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A modular cloning toolkit for genome editing in plants

BMC Plant Biology, 2020
Background CRISPR/Cas has recently become a widely used genome editing tool in various organisms, including plants. Applying CRISPR/Cas often requires delivering multiple expression units into plant and hence there is a need for a quick and easy cloning ...
Florian Hahn, Andrey Korolev, Laura Sanjurjo Loures, Vladimir Nekrasov   +3 more
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Hot and crispy : CRISPR-Cas systems in the hyperthermophile Sulfolobus solfataricus [PDF]

, 2013
The CRISPR (clustered regularly interspaced short palindromic repeats) and Cas (CRISPR-associated) genes are widely spread in bacteria and archaea, representing an intracellular defence system against invading viruses and plasmids.
White, Malcolm F., Zhang, Jing
core   +2 more sources

Action mechanisms of CRISPR/Cas system and its application in genetic improvement of crops

浙江大学学报. 农业与生命科学版, 2018
CRISPR/Cas system is an emerging gene-editing technology, which can knock out multiple genes at multiple specific loci. Compared with other gene editing systems including ZFNs (zinc finger nucleases) and TALENs (transcription activator-like effector ...
SHU Xinyuan, YAN Xu, PU Yehong, WANG Chao, PAN Jianwei   +4 more
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CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off‐Target Evaluation, and Strategies to Mitigate Off‐Target Effects

Advanced Science, 2020
Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory interspaced short palindromic repeats)/Cas (CRISPR‐associated) systems,
Hakim Manghwar, Bo Li, Xiao Ding, Amjad Hussain, Keith Lindsey, Xianlong Zhang, Shuangxia Jin   +6 more
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Advances in biotechnology: genomics and genome editing [PDF]

, 2017
Genomics, the study of genes, their functions and related techniques has become a crucial science for developing understanding of life processes and how they evolve.
Beccari, Tommaso, Dundar, Munis, Gartland, Jill S., Gartland, Kevan M.A., Magni, Mariapia Viola   +4 more
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Rewriting Human History and Empowering Indigenous Communities with Genome Editing Tools. [PDF]

, 2020
Appropriate empirical-based evidence and detailed theoretical considerations should be used for evolutionary explanations of phenotypic variation observed in the field of human population genetics (especially Indigenous populations). Investigators within
Fox, Keolu, Komor, Alexis C, Rallapalli, Kartik Lakshmi   +2 more
core   +2 more sources

Systematic identification of endogenous RNA polymerase III promoters for efficient RNA guide-based genome editing technologies in maize

Crop Journal, 2018
Single-guide RNA (sgRNA) is one of the two core components of the CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) genome-editing technology.
Xiantao Qi, Le Dong, Changlin Liu, Long Mao, Fang Liu, Xin Zhang, Beijiu Cheng, Chuanxiao Xie   +7 more
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