Development and Application of CRISPR/Cas in Microbial Biotechnology
Frontiers in Bioengineering and Biotechnology, 2020 The clustered regularly interspaced short palindromic repeats (CRISPR)-associated (Cas) system has been rapidly developed as versatile genomic engineering tools with high efficiency, accuracy and flexibility, and has revolutionized traditional methods ...
Wentao Ding +3 more
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Dynamic observations of CRISPR-Cas target recognition and cleavage heterogeneities
Nanophotonics, 2022 CRISPR-Cas systems (clustered regularly interspaced short palindromic repeats) have shown great potential as efficient gene editing tools in disease therapeutics.
Zhang Zhijia +12 more
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Structure of the Saccharolobus solfataricus type III-D CRISPR effector
Current Research in Structural Biology, 2023 CRISPR-Cas is a prokaryotic adaptive immune system, classified into six different types, each characterised by a signature protein. Type III systems, classified based on the presence of a Cas10 subunit, are rather diverse multi-subunit assemblies with a ...
Giuseppe Cannone +4 more
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Computational approaches for effective CRISPR guide RNA design and evaluation
Computational and Structural Biotechnology Journal, 2020 The Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/ CRISPR-associated (Cas) system has emerged as the main technology for gene editing. Successful editing by CRISPR requires an appropriate Cas protein and guide RNA.
Guanqing Liu, Yong Zhang, Tao Zhang
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CRISPR interference : a structural perspective [PDF]
, 2013 This article was made open access through BIS OA funding. The laboratory is funded by grants from the Biotechnology and Biological Sciences Research Council (BBSRC).CRISPR (cluster of regularly interspaced palindromic repeats) is a prokaryotic adaptive ...
Naismith, Jim +2 more
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A Type IV-A CRISPR-Cas System in Pseudomonas aeruginosa Mediates RNA-Guided Plasmid Interference In Vivo [PDF]
The CRISPR Journal, 2019 Bacteria and archaea use CRISPR-Cas adaptive immune systems to destroy complementary nucleic acids using RNAs derived from CRISPR loci. Here, we provide the first functional evidence for type IV CRISPR-Cas, demonstrating that the system from Pseudomonas aeruginosa strain PA83 mediates RNA ...
Valerie M, Crowley +6 more
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Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity
, 2015 EC is supported by the Alexander von Humboldt Foundation, the German Federal Ministry for Education and Research, the Helmholtz Association, the Göran Gustafsson Foundation, the Swedish Research Council, the Kempe Foundation and Umeå University.
White, Malcolm F. +4 more
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CRISPR/Cas-Based Diagnostics in Agricultural Applications
, 2023 Pests and disease-causing pathogens frequently impede agricultural production. An early and efficient diagnostic tool is crucial for effective disease management.
Nguyen, Nam-Trung +11 more
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Endogenous CRISPR-Cas System-Based Genome Editing and Antimicrobials: Review and Prospects
Frontiers in Microbiology, 2019 CRISPR-Cas systems adapt “memories” via spacers from viruses and plasmids to develop adaptive immunity against mobile genetic elements. Mature CRISPR RNAs guide CRISPR-associated nucleases to site-specifically cleave target DNA or RNA, providing an ...
Yingjun Li, Nan Peng
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The CRISPR‐Cas system: small RNA‐guided invader silencing in prokaryotes
The FASEB Journal, 2012 CRISPR‐Cas systems are recently discovered, RNA‐based immune systems that control invasions of viruses and plasmids in archaea and bacteria. Prokaryotes with CRISPR‐Cas immune systems capture short invader sequences within the CRISPR loci in their genomes, and produce small RNAs from the CRISPR loci (crRNAs) that recognize the invading nucleic acids ...
Becky Terns +7 more
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