Results 21 to 30 of about 22,098 (274)

A Rapid Hairy Root-Based Platform for CRISPR/Cas Optimization and Guide RNA Validation in Lettuce [PDF]

Plants
Cultivated lettuce (Lactuca sativa L.) is a major leafy crop and an emerging model for functional genomics within the Asteraceae family, supported by high-quality reference genomes and efficient transformation systems.
Alberico Di Pinto, Valentina Forte, Chiara D’Attilia, Marco Possenti, Barbara Felici, Floriana Augelletti, Giovanna Sessa, Monica Carabelli, Giorgio Morelli, Giovanna Frugis, Fabio D’Orso   +10 more
doaj   +2 more sources

Genome editing using CRISPR/ Cas9 system: a practical guide

Вавиловский журнал генетики и селекции, 2017
Over the past few years, the CRISPR/Cas techniques have become a revolution in genome editing. Since the original paper on CRIPSR/Cas9 genome editing, researches have proposed numerous modifications of the key components of the CRISPR/Cas9 system to make
A. G. Menzorov, V. A. Lukyanchikova, A. N. Korablev, I. A. Serova, V. S. Fishman   +4 more
doaj   +3 more sources

Guide RNAs: A Glimpse at the Sequences that Drive CRISPR–Cas Systems [PDF]

Cold Spring Harbor Protocols, 2016
CRISPR–Cas systems provide adaptive immunity in bacteria and archaea. Although there are two main classes of CRISPR–Cas systems defined by gene content, interfering RNA biogenesis, and effector proteins, Type II systems have recently been exploited on a broad scale to develop next-generation genetic engineering and genome-editing tools.
Alexandra E, Briner, Rodolphe, Barrangou
openaire   +2 more sources

Carrier strategies boost the application of CRISPR/Cas system in gene therapy

Exploration, 2022
Emerging clustered regularly interspaced short palindromic repeat/associated protein (CRISPR/Cas) genome editing technology shows great potential in gene therapy.
Zunkai Xu, Qingnan Wang, Haiping Zhong, Yaoyao Jiang, Xiaoguang Shi, Bo Yuan, Na Yu, Shubiao Zhang, Xiaoyong Yuan, Shutao Guo, Yang Yang   +10 more
doaj   +1 more source

RNA-guided editing of bacterial genomes using CRISPR-Cas systems [PDF]

Nature Biotechnology, 2013
Here we use the clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated Cas9 endonuclease complexed with dual-RNAs to introduce precise mutations in the genomes of Streptococcus pneumoniae and Escherichia coli. The approach relies on dual-RNA:Cas9-directed cleavage at the targeted genomic site to kill unmutated cells and ...
Jiang, Wenyan, Bikard, David, Cox, David Daniel, Zhang, Feng, Marraffini, Luciano A.   +4 more
openaire   +4 more sources

RNA-Guided Genome Editing in Plants Using a CRISPR–Cas System [PDF]

Molecular Plant, 2013
Precise and straightforward methods to edit the plant genome are much needed for functional genomics and crop improvement. Recently, RNA-guided genome editing using bacterial Type II cluster regularly interspaced short palindromic repeats (CRISPR)-associated nuclease (Cas) is emerging as an efficient tool for genome editing in microbial and animal ...
Xie, Kabin, Yang, Yinong
openaire   +2 more sources

CRISPR based therapeutics: a new paradigm in cancer precision medicine

Molecular Cancer, 2022
Background Clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein (Cas) systems are the latest addition to the plethora of gene-editing tools.
Sumit Das, Shehnaz Bano, Prachi Kapse, Gopal C. Kundu   +3 more
doaj   +1 more source

Functional Identification of the Xanthomonas oryzae pv. oryzae Type I-C CRISPR-Cas System and Its Potential in Gene Editing Application

Frontiers in Microbiology, 2021
The type I clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system is one of five adaptive immune systems and exists widely in bacteria and archaea. In this study, we showed that Xanthomonas oryzae pv.
Qibing Liu, Siwei Wang, Juying Long, Zhuoyue Chen, Bing Yang, Bing Yang, Fei Lin   +6 more
doaj   +1 more source

Target RNA-guided protease activity in type III-E CRISPR–Cas system

Nucleic Acids Research, 2022
Abstract The type III-E CRISPR–Cas systems are newly identified adaptive immune systems in prokaryotes that use a single Cas7–11 protein to specifically cleave target RNA. Cas7–11 could associate with Csx29, a putative caspase-like protein encoded by the gene frequently found in the type III-E loci, suggesting a functional linkage ...
Xiaoshen Wang, Guimei Yu, Yanan Wen, Qiyin An, Xuzichao Li, Fumeng Liao, Chengwei Lian, Kai Zhang, Hang Yin, Yong Wei, Zengqin Deng, Heng Zhang   +11 more
openaire   +2 more sources

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System [PDF]

Cell, 2015
The microbial adaptive immune system CRISPR mediates defense against foreign genetic elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize multi-protein complexes, whereas class 2 effectors rely on single-component effector proteins such as the well-characterized Cas9.
Zetsche, Bernd, Gootenberg, Jonathan S., Abudayyeh, Omar O., Slaymaker, Ian M., Makarova, Kira S., Essletzbichler, Patrick, Volz, Sara E., Joung, Julia, van der Oost, John, Regev, Aviv, Koonin, Eugene V., Zhang, Feng   +11 more
openaire   +4 more sources