Results 21 to 30 of about 22,577 (244)

Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems

Biology Direct, 2011
Background The CRISPR-Cas adaptive immunity systems that are present in most Archaea and many Bacteria function by incorporating fragments of alien genomes into specific genomic loci, transcribing the inserts and using the transcripts as guide RNAs to ...
Wolf Yuri I, Aravind L, Makarova Kira S, Koonin Eugene V   +3 more
doaj   +1 more source

Large scale screening of CRISPR guide RNAs using an optimized high throughput robotics system

Scientific Reports, 2022
All CRISPR/CAS systems utilize CRISPR guide RNAs (crRNAs), the design of which depend on the type of CAS protein, genetic target and the environment/matrix.
J. R. Spangler, T. A. Leski, Z. Schultzhaus, Z. Wang, D. A. Stenger   +4 more
doaj   +1 more source

The CRISPR‐Cas system: small RNA‐guided invader silencing in prokaryotes

The FASEB Journal, 2012
CRISPR‐Cas systems are recently discovered, RNA‐based immune systems that control invasions of viruses and plasmids in archaea and bacteria. Prokaryotes with CRISPR‐Cas immune systems capture short invader sequences within the CRISPR loci in their genomes, and produce small RNAs from the CRISPR loci (crRNAs) that recognize the invading nucleic acids ...
Becky Terns, Caryn Hale, Jason Carte, Joshua Elmore, Sonali Majumdar, Claiborne V.C. Glover, Brenton Graveley, Michael Terns   +7 more
openaire   +1 more source

In silico Analysis Suggests Common Appearance of scaRNAs in Type II Systems and Their Association With Bacterial Virulence

Frontiers in Genetics, 2018
In addition to its well-established defense function, CRISPR/Cas can also exhibit crucial non-canonical activity through endogenous gene expression regulation, which was found to mainly affect bacterial virulence.
Jelena Guzina, Jelena Guzina, Wei-Hua Chen, Tamara Stankovic, Magdalena Djordjevic, Evgeny Zdobnov, Marko Djordjevic   +6 more
doaj   +1 more source

A modular cloning toolkit for genome editing in plants

BMC Plant Biology, 2020
Background CRISPR/Cas has recently become a widely used genome editing tool in various organisms, including plants. Applying CRISPR/Cas often requires delivering multiple expression units into plant and hence there is a need for a quick and easy cloning ...
Florian Hahn, Andrey Korolev, Laura Sanjurjo Loures, Vladimir Nekrasov   +3 more
doaj   +1 more source

Action mechanisms of CRISPR/Cas system and its application in genetic improvement of crops

浙江大学学报. 农业与生命科学版, 2018
CRISPR/Cas system is an emerging gene-editing technology, which can knock out multiple genes at multiple specific loci. Compared with other gene editing systems including ZFNs (zinc finger nucleases) and TALENs (transcription activator-like effector ...
SHU Xinyuan, YAN Xu, PU Yehong, WANG Chao, PAN Jianwei   +4 more
doaj   +1 more source

CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off‐Target Evaluation, and Strategies to Mitigate Off‐Target Effects

Advanced Science, 2020
Life sciences have been revolutionized by genome editing (GE) tools, including zinc finger nucleases, transcription activator‐Like effector nucleases, and CRISPR (clustered regulatory interspaced short palindromic repeats)/Cas (CRISPR‐associated) systems,
Hakim Manghwar, Bo Li, Xiao Ding, Amjad Hussain, Keith Lindsey, Xianlong Zhang, Shuangxia Jin   +6 more
doaj   +1 more source

Systematic identification of endogenous RNA polymerase III promoters for efficient RNA guide-based genome editing technologies in maize

Crop Journal, 2018
Single-guide RNA (sgRNA) is one of the two core components of the CRISPR (clustered regularly interspaced short palindromic repeat)/Cas (CRISPR-associated) genome-editing technology.
Xiantao Qi, Le Dong, Changlin Liu, Long Mao, Fang Liu, Xin Zhang, Beijiu Cheng, Chuanxiao Xie   +7 more
doaj   +1 more source

Deep learning in CRISPR-Cas systems: a review of recent studies

Frontiers in Bioengineering and Biotechnology, 2023
In genetic engineering, the revolutionary CRISPR-Cas system has proven to be a vital tool for precise genome editing. Simultaneously, the emergence and rapid evolution of deep learning methodologies has provided an impetus to the scientific exploration ...
Minhyeok Lee
doaj   +1 more source

Genome editing using CRISPR, CAST, and Fanzor systems

Molecules and Cells
: Genetic engineering technologies are essential not only for basic science but also for generating animal models for therapeutic applications. The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein (Cas) system,
Beomjong Song, Sangsu Bae
doaj   +1 more source