Results 21 to 30 of about 34,514 (185)
Heritable genome editing in C. elegans via a CRISPR-Cas9 system [PDF]
, 2014 CRISPR-Cas systems have been used with single-guide RNAs for accurate gene disruption and conversion in multiple biological systems. Here we report the use of the endonuclease Cas9 to target genomic sequences in the C.
Calarco, John A. +5 more
core +1 more source
Endogenous CRISPR-Cas System-Based Genome Editing and Antimicrobials: Review and Prospects
Frontiers in Microbiology, 2019 CRISPR-Cas systems adapt “memories” via spacers from viruses and plasmids to develop adaptive immunity against mobile genetic elements. Mature CRISPR RNAs guide CRISPR-associated nucleases to site-specifically cleave target DNA or RNA, providing an ...
Yingjun Li, Nan Peng
doaj +1 more source
Using the CRISPR/Cas9 system to understand neuropeptide biology and regulation [PDF]
, 2016 Funding was provided by a Wellcome Trust ISSF starting grant (105625/Z/14/Z), Medical Research Scotland (PhD-719-2013), GW Pharmaceuticals (PhD-719-2013 - S.5242.001) and the BBSRC (BB/J012343/1).Peer reviewedPublisher ...
Hay, Elizabeth A +3 more
core +1 more source
Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoiling. [PDF]
, 2020 The CRISPR-Cas9 nuclease has been widely repurposed as a molecular and cell biology tool for its ability to programmably target and cleave DNA. Cas9 recognizes its target site by unwinding the DNA double helix and hybridizing a 20-nucleotide section of ...
Aris, Kevin D Palacio +5 more
core +3 more sources
Large scale screening of CRISPR guide RNAs using an optimized high throughput robotics system
Scientific Reports, 2022 All CRISPR/CAS systems utilize CRISPR guide RNAs (crRNAs), the design of which depend on the type of CAS protein, genetic target and the environment/matrix.
J. R. Spangler +4 more
doaj +1 more source
Boosting plant immunity with CRISPR/Cas [PDF]
, 2015 CRISPR/Cas has recently been transferred to plants to make them resistant to geminiviruses, a damaging family of DNA viruses. We discuss the potential and the limitations of this method.See related Research: http://www.genomebiology.com/2015/16/1 ...
Chaparro-Garcia, Angela +2 more
core +1 more source
The CRISPR‐Cas system: small RNA‐guided invader silencing in prokaryotes
The FASEB Journal, 2012 CRISPR‐Cas systems are recently discovered, RNA‐based immune systems that control invasions of viruses and plasmids in archaea and bacteria. Prokaryotes with CRISPR‐Cas immune systems capture short invader sequences within the CRISPR loci in their genomes, and produce small RNAs from the CRISPR loci (crRNAs) that recognize the invading nucleic acids ...
Becky Terns +7 more
openaire +1 more source
CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks
eLife, 2020 Type V CRISPR-Cas interference proteins use a single RuvC active site to make RNA-guided breaks in double-stranded DNA substrates, an activity essential for both bacterial immunity and genome editing.
Joshua C Cofsky +5 more
doaj +1 more source
Genome editing technologies to fight infectious diseases [PDF]
, 2017 Genome editing by programmable nucleases represents a promising tool that could be exploited to develop new therapeutic strategies to fight infectious diseases.
Barzon, Luisa +2 more
core +1 more source
Biology Direct, 2011 Background The CRISPR-Cas adaptive immunity systems that are present in most Archaea and many Bacteria function by incorporating fragments of alien genomes into specific genomic loci, transcribing the inserts and using the transcripts as guide RNAs to ...
Wolf Yuri I +3 more
doaj +1 more source