Results 21 to 30 of about 3,929,879 (390)

Efficient plant genome engineering using a probiotic sourced CRISPR-Cas9 system

Nature Communications, 2023
Among CRISPR-Cas genome editing systems, Streptococcus pyogenes Cas9 (SpCas9), sourced from a human pathogen, is the most widely used. Here, through in silico data mining, we have established an efficient plant genome engineering system using CRISPR-Cas9
Z. Zhong, Guanqing Liu, Zhongjie Tang, Shuyue Xiang, Liang Yang, Lan Huang, Yao He, T. Fan, Shishi Liu, Xuelian Zheng, Tao Zhang, Yiping Qi, Jian Huang, Yong Zhang   +13 more
semanticscholar   +1 more source

A genome engineering resource to uncover principles of cellular organization and tissue architecture by lipid signaling

eLife, 2020
Phosphoinositides (PI) are key regulators of cellular organization in eukaryotes and genes that tune PI signaling are implicated in human disease mechanisms.
Deepti Trivedi, Vinitha CM, Karishma Bisht, Vishnu Janardan, Awadhesh Pandit, Bishal Basak, Shwetha H, Navyashree Ramesh, Padinjat Raghu   +8 more
doaj   +1 more source

Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems

Nature Communications, 2021
CRISPR-Cas12a is a promising genome editing system for targeting AT-rich genomic regions. Comprehensive genome engineering requires simultaneous targeting of multiple genes at defined locations.
Yingxiao Zhang, Qiurong Ren, Xu Tang, Shishi Liu, Aimee A. Malzahn, Jianping Zhou, Jiaheng Wang, Desuo Yin, Changtian Pan, Mingzhu Yuan, Lan Huang, Han Yang, Yuxin Zhao, Qing Fang, Xuelian Zheng, L. Tian, Yanhao Cheng, Ysa Le, Bailey McCoy, Lidiya Franklin, J. Selengut, Stephen M. Mount, Q. Que, Yong Zhang, Yiping Qi   +24 more
semanticscholar   +1 more source

Tree Genetic Engineering, Genome Editing and Genomics

International Journal of Molecular Sciences, 2022
In this Special Issue [...]
Matthias Fladung, Birgit Kersten
openaire   +2 more sources

Potential Application of the CRISPR/Cas9 System against Herpesvirus Infections. [PDF]

, 2018
The CRISPR/Cas9 system has been applied in the genome editing and disruption of latent infections for herpesviruses such as the herpes simplex virus, Epstein⁻Barr virus, cytomegalovirus, and Kaposi's sarcoma-associated herpesvirus.
Chen, Yuan-Chuan, Liu, Fenyong, Sheng, Jingxue, Trang, Phong   +3 more
core   +5 more sources

Live pigs produced from genome edited zygotes [PDF]

, 2013
Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome.
AJ Clark, AM Guerts, CJ Palgrave, D Yang, DF Carlson, FD Urnov, ID Carbery, J Hauschild, JC Miller, JJ Whyte, SC Fahrenkrug, T Flisokowska, VM Bedell, WS Tan   +13 more
core   +1 more source

The Promising Nanovectors for Gene Delivery in Plant Genome Engineering

International Journal of Molecular Sciences, 2022
Highly efficient gene delivery systems are essential for genetic engineering in plants. Traditional delivery methods have been widely used, such as Agrobacterium-mediated transformation, polyethylene glycol (PEG)-mediated delivery, biolistic particle ...
Heng Zhi, Shengen Zhou, Wenbo Pan, Yun Shang, Zhanghua Zeng, Huawei Zhang   +5 more
semanticscholar   +1 more source

Investigation of Genome Biology by Synthetic Genome Engineering

Bioengineering, 2023
Synthetic genomes were designed based on an understanding of natural genomic information, offering an opportunity to engineer and investigate biological systems on a genome-wide scale. Currently, the designer version of the M.
Hui Zhang, Yao Xiong, Wenhai Xiao, Yi Wu
doaj   +1 more source

Precision genome engineering [PDF]

Current Biology, 2014
What is precision genome engineering? Exactly that! You know what a genome is; ‘engineering’ means that we want to change that DNA, and ‘precision’ means we want to do it in a very specific, targeted way.Sounds challenging, why would you want to do that? Lots of reasons.
openaire   +2 more sources

Efficient CRISPR/Cas9-Mediated Genome Editing Using a Chimeric Single-Guide RNA Molecule

Frontiers in Plant Science, 2017
The CRISPR/Cas9 system has been applied in diverse eukaryotic organisms for targeted mutagenesis. However, targeted gene editing is inefficient and requires the simultaneous delivery of a DNA template for homology-directed repair (HDR).
Haroon Butt, Ayman Eid, Zahir Ali, Mohamed A. M. Atia, Morad M. Mokhtar, Norhan Hassan, Ciaran M. Lee, Gang Bao, Magdy M. Mahfouz   +8 more
doaj   +1 more source