Cross-talk between Diverse Serine Integrases [PDF]
Journal of Molecular Biology, 2014 Phage-encoded serine integrases are large serine recombinases that mediate integrative and excisive site-specific recombination of temperate phage genomes. They are well suited for use in heterologous systems and for synthetic genetic circuits as the attP and attB attachment sites are small (
Shweta, Singh +4 more
openaire +2 more sources
Site‐specificity of serine integrase demonstrated by the attB sequence preference of ɸBT1 integrase [PDF]
FEBS Letters, 2018 Serine integrases mediate site‐specific recombination and are extensively applied in genetic engineering and synthetic biology. However, which regions of the attachment sites determine site‐specificity and how these regions function in recombination remain elusive.
Xiaolai Lei +3 more
semanticscholar +3 more sources
Tight regulation of the intS gene of the KplE1 prophage: a new paradigm for integrase gene regulation. [PDF]
PLoS Genetics, 2010 Temperate phages have the ability to maintain their genome in their host, a process called lysogeny. For most, passive replication of the phage genome relies on integration into the host's chromosome and becoming a prophage.
Gaël Panis +5 more
doaj +1 more source
The ΦBT1 large serine recombinase catalyzes DNA integration at pseudo-attB sites in the genus Nocardia [PDF]
PeerJ, 2018 Plasmid vectors based on bacteriophage integrases are important tools in molecular microbiology for the introduction of foreign DNA, especially into bacterial species where other systems for genetic manipulation are limited.
Marion Herisse +8 more
doaj +2 more sources
Mutational analysis of highly conserved residues in the phage phiC31 integrase reveals key amino acids necessary for the DNA recombination. [PDF]
PLoS ONE, 2010 BACKGROUND: Amino acid sequence alignment of phage phiC31 integrase with the serine recombinases family revealed highly conserved regions outside the catalytic domain. Until now, no system mutational or biochemical studies have been carried out to assess
Shaohui Liu +7 more
doaj +1 more source
Imaging cell lineage with a synthetic digital recording system [PDF]
, 2020 Cell lineage plays a pivotal role in cell fate determination. Chow et al. demonstrate the use of an integrase-based synthetic barcode system called intMEMOIR, which uses the serine integrase Bxb1 to perform irreversible nucleotide edits.
Budde, Mark W. +11 more
core +1 more source
PLoS ONE, 2022 The threat to public health posed by drug-resistant bacteria is rapidly increasing, as some of healthcare's most potent antibiotics are becoming obsolete.
Elizabeth T Montaño +40 more
doaj +2 more sources
Control of phage Bxb1 excision by a novel recombination directionality factor. [PDF]
PLoS Biology, 2006 Mycobacteriophage Bxb1 integrates its DNA at the attB site of the Mycobacterium smegmatis genome using the viral attP site and a phage-encoded integrase generating the recombinant junctions attL and attR.
Pallavi Ghosh +2 more
doaj +1 more source
High fidelity one‐pot DNA assembly using orthogonal serine integrases
Biotechnology Journal, 2022 AbstractBackgroundLarge serine integrases (LSIs, derived from temperate phages) have been adapted for use in a multipart DNA assembly process in vitro, called serine integrase recombinational assembly (SIRA). The versatility, efficiency, and fidelity of SIRA is limited by lack of a sufficient number of LSIs whose activities have been characterized in ...
Jumai Abioye +7 more
openaire +2 more sources
Control of directionality in Streptomyces phage φBT1 integrase-mediated site-specific recombination. [PDF]
PLoS ONE, 2013 Streptomyces phage φBT1 integrates its genome into the attB site of the host chromosome with the attP site to generate attL and attR. The φBT1 integrase belongs to the large serine recombinase subfamily which directly binds to target sites to initiate ...
Lin Zhang +4 more
doaj +1 more source