Results 41 to 50 of about 6,315 (155)
Site‐specificity of serine integrase demonstrated by the attB sequence preference of ɸ
FEBS Letters, 2018 Serine integrases mediate site‐specific recombination and are extensively applied in genetic engineering and synthetic biology. However, which regions of the attachment sites determine site‐specificity and how these regions function in recombination remain elusive.
Xiaolai, Lei +3 more
openaire +2 more sources
Identification of the Integration/Excision Module and Regulatory Elements Involved in the Mobility of IME8, an Integrative and Mobilizable Element From Mosquitocidal Lysinibacillus sphaericus. [PDF]
Microb BiotechnolMosquitocidal genomic island IME8 carries an int‐operon essential for integration/excision and a reg‐operon activating the promoter Pint and repressing excision. Its integration target is present in L. sphaericus and beyond. IME8 and a mini‐IME8 cassette can be mobilized by a conjugative plasmid and display integrating properties into the specific ...
Hu Y +5 more
europepmc +2 more sources
Scientific Reports, 2022 Streptomycetes are ubiquitous soil bacteria. Here we report the complete and annotated genome sequence and characterization of Streptomyces phage Pablito, isolated from a soil sample in Haarlem, the Netherlands using Streptomyces lividans as host.
Véronique Ongenae +5 more
doaj +1 more source
High fidelity one‐pot DNA assembly using orthogonal serine integrases
Biotechnology Journal, 2022 AbstractBackgroundLarge serine integrases (LSIs, derived from temperate phages) have been adapted for use in a multipart DNA assembly process in vitro, called serine integrase recombinational assembly (SIRA). The versatility, efficiency, and fidelity of SIRA is limited by lack of a sufficient number of LSIs whose activities have been characterized in ...
Jumai Abioye +7 more
openaire +2 more sources
Mechanistic modelling of a recombinase-based two-input temporal logic gate
Engineering Biology, 2017 Site-specific recombinases (SSRs) mediate efficient manipulation of DNA sequences in vitro and in vivo. In particular, serine integrases have been identified as highly effective tools for facilitating DNA inversion, enabling the design of genetic ...
Jack E. Bowyer +3 more
doaj +1 more source
SHIELD: a platform for high-throughput screening of barrier-type DNA elements in human cells
Nature Communications, 2023 Chromatin boundary elements contribute to the partitioning of mammalian genomes into topological domains to regulate gene expression. Certain boundary elements are adopted as DNA insulators for safe and stable transgene expression in mammalian cells ...
Meng Zhang +5 more
doaj +1 more source
Emerging Species and Genome Editing Tools: Future Prospects in Cyanobacterial Synthetic Biology
Microorganisms, 2019 Recent advances in synthetic biology and an emerging algal biotechnology market have spurred a prolific increase in the availability of molecular tools for cyanobacterial research.
Grant A. R. Gale +5 more
doaj +1 more source
Frontiers in Cellular and Infection Microbiology, 2021 Bacteriophages (phages) are viruses that infect bacteria. They are the most abundant biological entity on Earth (current estimates suggest there to be perhaps 1031 particles) and are found nearly everywhere.
Antonio J. Martín-Galiano +2 more
doaj +1 more source
Identification of cognate recombination directionality factors for large serine recombinases by virtual pulldown. [PDF]
Nucleic Acids ResIntegrases from the "large serine"family are simple, highly directional site-specific DNA recombinases that have great promise as synthetic biology and genome editing tools. Integrative recombination (mimicking phage or mobile element insertion) requires
Shin H +11 more
europepmc +2 more sources
Applications of Serine Integrases in Synthetic Biology over the Past Decade
SynBio, 2023 Serine integrases are emerging as one of the most powerful biological tools for biotechnology. Over the past decade, many research papers have been published on the use of serine integrases in synthetic biology. In this review, we aim to systematically summarize the various studies ranging from structure and the catalytic mechanism to genetic design ...
Fang Ba +4 more
openaire +1 more source