Results 51 to 60 of about 217,395 (184)

Measurement of large serine integrase enzymatic characteristics in HEK293 cells reveals variability and influence on downstream reporter expression

The FEBS Journal, 2021
Large serine integrases (LSIs) offer tremendous potential for rapid genetic engineering as well as building biological systems capable of responding to stimuli and integrating information.
George Chao, Clair Travis, George Church
semanticscholar   +1 more source

Two-step site selection for serine-integrase-mediated excision: DNA-directed integrase conformation and central dinucleotide proofreading [PDF]

Proceedings of the National Academy of Sciences, 2008
Bacteriophage-encoded serine-integrases are members of the large family of serine-recombinases and catalyze site-specific integrative recombination between a phage attP site and a bacterial attB site to form an integrated prophage.
P. Ghosh, Lori A. Bibb, G. Hatfull
semanticscholar   +3 more sources

Genetic switches designed for eukaryotic cells and controlled by serine integrases [PDF]

Communications Biology, 2020
AbstractRecently, new serine integrases have been identified, increasing the possibility of scaling up genomic modulation tools. Here, we describe the use of unidirectional genetic switches to evaluate the functionality of six serine integrases in different eukaryotic systems: the HEK 293T cell lineage, bovine fibroblasts and plant protoplasts ...
Mayna S. Gomide, Thais T. Sales, Luciana R. C. Barros, Cintia G. Limia, Marco A. de Oliveira, Lilian H. Florentino, Leila M. G. Barros, Maria L. Robledo, Gustavo P. C. José, Mariana S. M. Almeida, Rayane N. Lima, Stevens K. Rehen, Cristiano Lacorte, Eduardo O. Melo, André M. Murad, Martín H. Bonamino, Cintia M. Coelho, Elibio Rech   +17 more
openaire   +2 more sources

Controlled rotation mechanism of DNA strand exchange by the Hin serine recombinase. [PDF]

, 2016
DNA strand exchange by serine recombinases has been proposed to occur by a large-scale rotation of halves of the recombinase tetramer. Here we provide the first direct physical evidence for the subunit rotation mechanism for the Hin serine invertase ...
Johnson, Reid C, Lei, Xianbin, Marko, John F, McLean, Meghan M, Xiao, Botao   +4 more
core   +1 more source

Multiple interfaces between a serine recombinase and an enhancer control site-specific DNA inversion. [PDF]

, 2013
Serine recombinases are often tightly controlled by elaborate, topologically-defined, nucleoprotein complexes. Hin is a member of the DNA invertase subclass of serine recombinases that are regulated by a remote recombinational enhancer element containing
Chang, Yong, Dhar, Gautam, Heiss, John K, Johnson, Reid C, McLean, Meghan M   +4 more
core   +1 more source

Large serine integrases utilise scavenged phage proteins as directionality cofactors. [PDF]

Nucleic Acids Res
Abstract Recombination directionality factors (RDFs) for large serine integrases (LSIs) are cofactor proteins that control the directionality of recombination to favour excision over insertion. Although RDFs are predicted to bind their cognate LSIs in similar ways, there is no overall common structural theme across LSI RDFs, leading ...
Alsaleh A, Holland A, Shin H, Reyes TP, Baksh A, Taiwo-Aiyerin OT, Pigli Y, Rice PA, Olorunniji FJ.   +8 more
europepmc   +4 more sources

Controlling tetramer formation, subunit rotation and DNA ligation during Hin-catalyzed DNA inversion. [PDF]

, 2015
Two critical steps controlling serine recombinase activity are the remodeling of dimers into the chemically active synaptic tetramer and the regulation of subunit rotation during DNA exchange.
Chang, Yong, Johnson, Reid C
core   +3 more sources

Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus

Scientific Reports, 2022
Streptomycetes are ubiquitous soil bacteria. Here we report the complete and annotated genome sequence and characterization of Streptomyces phage Pablito, isolated from a soil sample in Haarlem, the Netherlands using Streptomyces lividans as host.
Véronique Ongenae, Joana Azeredo, Andrew M. Kropinski, Daniel Rozen, Ariane Briegel, Dennis Claessen   +5 more
doaj   +1 more source

GCN2 phosphorylates HIV-1 integrase and decreases HIV-1 replication by limiting viral integration

Scientific Reports, 2017
GCN2 is a serine/threonine kinase involved in cellular stress response related to amino acid starvation. Previously, we showed that GCN2 interacts with HIV-1 integrase and is activated during HIV-1 infection.
A. Jaspart, C. Calmels, O. Cosnefroy, P. Bellecave, P. Pinson, S. Claverol, V. Guyonnet-Dupérat, B. Dartigues, M. S. Benleulmi, E. Mauro, P. A. Gretteau, V. Parissi, M. Métifiot, M. L. Andreola   +13 more
doaj   +1 more source

Machines on Genes: Enzymes that Make, Break and Move DNA and RNA [PDF]

, 2010
As the vital information repositories of the cell, the nucleic acids DNA and RNA pose many challenges as enzyme substrates. To produce, maintain and repair DNA and RNA, and to extract the genetic information that they encode, a battery of remarkable ...
Bates, Belousoff, Ben F. Luisi, Lilley, Marshall, Olorunniji, Rice, Richard P. Bowater, Sherratt, Smith, Szczelkun, Tomlinson, W. Marshall Stark, Weinzierl   +13 more
core   +1 more source