Results 71 to 80 of about 217,395 (184)

Extreme C‐terminal element of SprA serine integrase is a potential component of the “molecular toggle switch” which controls the recombination and its directionality

Molecular Microbiology, 2020
In Bacillus subtilis, a sporulation‐related gene, spsM, is disrupted by SPβ prophage, but reconstituted during sporulation through SPβ excision. The spsM reconstitution is catalyzed by a site‐specific DNA recombinase, SprA, and its cognate recombination ...
Kimihiro Abe, Takumi Takahashi, Tsutomu Sato   +2 more
semanticscholar   +1 more source

Reprogrammed Serine Integrases Enable Precise Integration of Synthetic DNA

bioRxiv
Summary Despite recent progress in the ability to manipulate the genomes of eukaryotic cells1–3, there is still no effective and practical method to precisely integrate large synthetic DNA constructs into desired chromosomal sites using a programmable integrase. Serine integrases can perform the necessary molecular steps4, but only if their
Friedrich Fauser, Sebastian Arangundy-Franklin, Jessica E Davis, Lifeng Liu, Nicola J Schmidt, Luis Rodriguez, Danny F. Xia, N. Nguyen, Nicholas A Scarlott, Rakshaa Mureli, Irene Tan, Yuanyue Zhou, Lynn N. Truong, Sarah Hinkley, Bhakti N Kadam, Stephen C. Lam, Bryan C. Bourgeois, Emily Tait, Mohammad Qasim, Vishvesha Vaidya, A. Chen, Andrew Nguyen, Yuri R. Bendaña, David A. Shivak, P. Li, A. Reik, David E. Paschon, Gregory D Davis, Jeffrey C. Miller   +28 more
semanticscholar   +2 more sources

Repressing Integrase attachment site operation with CRISPR-Cas9 in E. coli [PDF]

, 2017
Serine integrases are bacteriophage proteins responsible for integrating the phage genome into that of the host. Synthetic biologists have co-opted these proteins into useful tools for permanent DNA logic, utilizing their specific DNA recombination ...
Murray, Richard M., Shur, Andrey
core   +1 more source

Applications of Serine Integrases in Synthetic Biology over the Past Decade

SynBio, 2023
Serine integrases are emerging as one of the most powerful biological tools for biotechnology. Over the past decade, many research papers have been published on the use of serine integrases in synthetic biology. In this review, we aim to systematically summarize the various studies ranging from structure and the catalytic mechanism to genetic design ...
Fang Ba, Yufei Zhang, Luyao Wang, Wan-Qiu Liu, Jian Li   +4 more
openaire   +1 more source

A population-based temporal logic gate for timing and recording chemical events [PDF]

, 2016
Engineered bacterial sensors have potential applications in human health monitoring, environmental chemical detection, and materials biosynthesis. While such bacterial devices have long been engineered to differentiate between combinations of inputs ...
Hori, Yutaka, Hsiao, Victoria, Murray, Richard M., Rothemund, Paul W. K.   +3 more
core   +2 more sources

Recombination directionality factor gp3 binds ϕC31 integrase via the zinc domain, potentially affecting the trajectory of the coiled-coil motif [PDF]

, 2017
To establish a prophage state, the genomic DNA of temperate bacteriophages normally becomes integrated into the genome of their host bacterium by integrase-mediated, site-specific DNA recombination.
Fernando, Booshini D, Fogg, Paul C M, Khaleel, Thanafez, Smith, Margaret C M, Stark, W Marshall, Younger, Ellen   +5 more
core   +2 more sources

Alternating High‐Fat and Polysaccharide Diets Modulates Gut Phage‐Bacterial Interplay

Advanced Science, EarlyView.
This study reveals how alternating high‐fat and polysaccharide diets reshape the human gut virome and enhance phage‐bacteria interactions. Using large‐scale metagenomic meta‐analysis and a time‐resolved mouse model, the authors show that diets strongly modulate phage abundance, lifestyle, and gene exchange, offering new insights into nutrition‐guided ...
Fengxiang Zhao, Ruiqi Zhang, Rujun Wei, Huimin Fan, Yongfei Hu, Wenyu Shi, Jinfeng Wang   +6 more
wiley   +1 more source

Permanent genetic memory with >1-byte capacity [PDF]

, 2014
Genetic memory enables the recording of information in the DNA of living cells. Memory can record a transient environmental signal or cell state that is then recalled at a later time.
Fernandez-Rodriguez, Jesus, Laub, Michael T, Lu, Timothy K, McClune, Conor James, Nielsen, Alec Andrew, Voigt, Christopher A., Yang, Lei   +6 more
core   +4 more sources

Large Serine Integrase Off-Target Discovery with Deep Learning for Genome Wide Prediction

bioRxiv
Large Serine Integrases (LSIs) hold significant therapeutic promise due to their ability to efficiently incorporate gene-sized DNA into the human genome, offering a method to integrate healthy genes in patients with monogenic disorders or to insert gene ...
Matthew H. Bakalar, Thomas Biondi, Xiaoyu Liang, Didac Santesmasses, Anne M. Bara, Japan B. Mehta, Jie Wang, Dane Z. Hazelbaker, Jonathan D. Finn, Daniel J. O’Connell   +9 more
semanticscholar   +1 more source

Intracellular Aβ42 Sequestration by a Serine Protease Mitigates Neurotoxicity in a Drosophila Alzheimer's Disease Model

Advanced Science, EarlyView.
Emerging evidence suggests that intraneuronal Aβ accumulation represents an early pathogenic event in Alzheimer's disease (AD). Using Drosophila AD model, this study shows that a nonsecreted serine protease Yip7 physically interacts with Aβ. This causes intraneuronal Aβ accumulation but surprisingly reduces the associated neurotoxicity, arguing that ...
Jingyun Su, Meng Yang, Xinfeng Wang, Pa Wu, Zongzhao Zhai   +4 more
wiley   +1 more source