Results 61 to 70 of about 6,315 (155)
Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination. [PDF]
Nucleic Acids Res, 2014 Abstract Synthetic biology requires effective methods to assemble DNA parts into devices and to modify these devices once made. Here we demonstrate a convenient rapid procedure for DNA fragment assembly using site-specific recombination by ϕC31 integrase.
Colloms SD +7 more
europepmc +6 more sources
Acta Crystallographica Section D, Volume 82, Issue 6, Page 587-602, June 2026.This review describes how an integrated structural biology platform at Merck Sharp & Dohme LLC, Rahway, New Jersey, USA, combining X‐ray crystallography, cryo‐EM, MicroED and cryo‐ET, enables seamless three‐dimensional insights from molecular to cellular length scales.
Jill E. Chrencik +44 more
wiley +1 more source
Alternating High‐Fat and Polysaccharide Diets Modulates Gut Phage‐Bacterial Interplay
Advanced Science, Volume 13, Issue 29, 22 May 2026.This study reveals how alternating high‐fat and polysaccharide diets reshape the human gut virome and enhance phage‐bacteria interactions. Using large‐scale metagenomic meta‐analysis and a time‐resolved mouse model, the authors show that diets strongly modulate phage abundance, lifestyle, and gene exchange, offering new insights into nutrition‐guided ...
Fengxiang Zhao +6 more
wiley +1 more source
Sequencing and analysis of the DNA genome of the temperate bacteriophage Aa[Phi]23 of "Actinobacillus actinomycetemcomitans" [PDF]
, 2005 The entire genomic sequence of the AaΦ23 bacteriophage is presented. Its size is 43,033 bp and it has an overall molar G+C content of 41mol%. 57 potential open reading frames (ORFs) were identified.
Resch, Grégory
core +1 more source
Advanced Science, Volume 13, Issue 26, 8 May 2026.Emerging evidence suggests that intraneuronal Aβ accumulation represents an early pathogenic event in Alzheimer's disease (AD). Using Drosophila AD model, this study shows that a nonsecreted serine protease Yip7 physically interacts with Aβ. This causes intraneuronal Aβ accumulation but surprisingly reduces the associated neurotoxicity, arguing that ...
Jingyun Su +4 more
wiley +1 more source
Control of ϕC31 integrase-mediated site-specific recombination by protein trans-splicing. [PDF]
, 2019 Serine integrases are emerging as core tools in synthetic biology and have applications in biotechnology and genome engineering. We have designed a split-intein serine integrase-based system with potential for regulation of site-specific recombination ...
Stark, W. Marshall +16 more
core +1 more source
Protein-Mediated and RNA-Based Origins of Replication of Extrachromosomal Mycobacterial Prophages
mBio, 2020 Temperate bacteriophages are common and establish lysogens of their bacterial hosts in which the prophage is stably inherited. It is typical for such prophages to be integrated into the bacterial chromosome, but extrachromosomally replicating prophages ...
Katherine S. Wetzel +4 more
doaj +1 more source
Multi‐Targeting Non‐Specific Genome Engineering in Bacteria
Advanced Science, Volume 13, Issue 25, 4 May 2026.In this study, we provide the first case to use the multi‐targeting integrase (MTI) systems in bacteria and develop a host‐independent generalizable approach, MNGE (Multi‐targeting Non‐specific Genome Engineering), for multi‐copy and random integration of metabolic genes or pathways in both Gram‐positive and Gram‐negative bacteria, which will ...
Runze Sun +7 more
wiley +1 more source
PLoS Pathogens, 2019 The causative mutation responsible for limb girdle muscular dystrophy 1F (LGMD1F) is one heterozygous single nucleotide deletion in the stop codon of the nuclear import factor Transportin 3 gene (TNPO3).
Sara Rodríguez-Mora +13 more
doaj +1 more source
Requirement for a conserved serine in both processing and joining activities of retroviral integrase. [PDF]
Proceedings of the National Academy of Sciences, 1992 Retroviruses encode a protein, the integrase (IN), that is required for insertion of the viral DNA into the host cell chromosome. IN alone can carry out the integration reaction in vitro. The reaction involves endonucleolytic cleavage near the 3' ends of both viral DNA strands (the processing step), followed by joining of these new viral DNA ends to ...
R A, Katz +6 more
openaire +2 more sources