Open-source genomic analysis of Shiga-toxin–producing E. coli O104:H4 [PDF]
, 2011 An outbreak caused by Shiga-toxin–producing Escherichia coli O104:H4 occurred in Germany in May and June of 2011, with more than 3000 persons infected. Here, we report a cluster of cases associated with a single family and describe an open-source genomic
Antoine Danchin +39 more
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Attachment site recognition and regulation of directionality by the serine integrases [PDF]
Nucleic Acids Research, 2013 Abstract Serine integrases catalyze the integration of bacteriophage DNA into a host genome by site-specific recombination between ‘attachment sites’ in the phage ( attP ) and the host ( attB ). The reaction is highly directional; the reverse excision reaction between the product attL and attR sites does not occur in the absence of a ...
Rutherford, Karen +4 more
openaire +2 more sources
SHIELD: a platform for high-throughput screening of barrier-type DNA elements in human cells
Nature Communications, 2023 Chromatin boundary elements contribute to the partitioning of mammalian genomes into topological domains to regulate gene expression. Certain boundary elements are adopted as DNA insulators for safe and stable transgene expression in mammalian cells ...
Meng Zhang +5 more
doaj +1 more source
Anti-phage islands force their target phage to directly mediate island excision and spread. [PDF]
, 2018 Vibrio cholerae, the causative agent of the diarrheal disease cholera, is antagonized by the lytic phage ICP1 in the aquatic environment and in human hosts. Mobile genetic elements called PLEs (phage-inducible chromosomal island-like elements) protect V.
McKitterick, Amelia C, Seed, Kimberley D
core +1 more source
Gated rotation mechanism of site-specific recombination by ϕC31 integrase [PDF]
, 2012 Integrases, such as that of the Streptomyces temperate bacteriophage ϕC31, promote site-specific recombination between DNA sequences in the bacteriophage and bacterial genomes to integrate or excise the phage DNA.
A. R. McEwan +28 more
core +1 more source
Frontiers in Cellular and Infection Microbiology, 2021 Bacteriophages (phages) are viruses that infect bacteria. They are the most abundant biological entity on Earth (current estimates suggest there to be perhaps 1031 particles) and are found nearly everywhere.
Antonio J. Martín-Galiano +2 more
doaj +1 more source
The mechanism of ϕC31 integrase directionality : experimental analysis and computational modelling [PDF]
, 2016 Serine integrases, DNA site-specific recombinases used by bacteriophages for integration and excision of their DNA to and from their host genomes, are increasingly being used as tools for programmed rearrangements of DNA molecules for biotechnology and ...
Colloms, Sean D +5 more
core +3 more sources
Genome integration and excision by a new Streptomyces bacteriophage, ϕJoe [PDF]
, 2017 Bacteriophages are the source of many valuable tools for molecular biology and genetic manipulation. In Streptomyces, most DNA cloning vectors are based on serine integrase site-specific DNA recombination systems derived from phage.
Fogg, Paul C M +3 more
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Critical involvement of the ATM-dependent DNA damage response in the apoptotic demise of HIV-1-elicited syncytia. [PDF]
PLoS ONE, 2008 DNA damage can activate the oncosuppressor protein ataxia telangiectasia mutated (ATM), which phosphorylates the histone H2AX within characteristic DNA damage foci.
Jean-Luc Perfettini +20 more
doaj +1 more source
Targeting STAT3 in Cancer with Nucleotide Therapeutics. [PDF]
, 2019 Signal transducer and activator of transcription 3 (STAT3) plays a critical role in promoting the proliferation and survival of tumor cells. As a ubiquitously-expressed transcription factor, STAT3 has commonly been considered an "undruggable" target for ...
Grandis, Jennifer R +3 more
core +2 more sources